LOCUS       sequence-67057-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Maier CJ, Maier RH, Hintner H, Bauer JW, Onder K
  TITLE     Coupled yeast 2-hybrid-mammalian 2-hybrid reading-frame-independent 
            and site-specific recombinational cloning vector system.
  JOURNAL   Assay Drug Dev Technol. 2010 Oct;8(5):625-9. doi: 
            10.1089/adt.2009.0266. Epub 2010 Jun 1.
  PUBMED    20515414
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/adt.2009.0266"; journalName: "Assay Drug Dev Technol"; 
            date: "2010-10"; volume: "8"; issue: "5"; pages: "625-9"
COMMENT     Sequence Label: pMBD-Gate6
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    47..171
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(212..517)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(314..333)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tcgccggaat tcccggggat ccgtcgactt gacgcgttga tatcgaacca ctttgtacaa
       61 gaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca
      121 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggtcgacctg
      181 cagactggct gtgtataagg gagcctgaca tttatattcc ccagaacatc aggttaatgg
      241 cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg ataacggaga
      301 ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg atatgcacca
      361 ccgggtaaag ttcacggggg actttatctg acagcagacg tgcactggcc agggggatca
      421 ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac agacgataac
      481 ggctctctct tttataggtg taaaccttaa actgcatttc accagcccct gttctcgtca
      541 gcaaaagagc cgttcatttc aataaaccgg gcgacctcag ccatcccttc ctgattttcc
      601 gctttccagc gttcggcacg cagacgacgg gcttcattct gcatggttgt gcttaccaga
      661 ccggagatat tgacatcata tatgccttga gcaactgata gctgtcgctg tcaactgtca
      721 ctgtaatacg ctgcttcata gcatacctct ttttgacata cttcgggtat acatatcagt
      781 atatattctt ataccgcaaa aatcagcgcg caaatacgca tactgttatc tggcttttag
      841 taagccggat cctctagatt acgccccgcc tgccactcat cgcagtactg ttgtaattca
      901 ttaagcattc tgccgacatg gaagcc
//
