LOCUS       sequence-67058-e        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Maier CJ, Maier RH, Hintner H, Bauer JW, Onder K
  TITLE     Coupled yeast 2-hybrid-mammalian 2-hybrid reading-frame-independent 
            and site-specific recombinational cloning vector system.
  JOURNAL   Assay Drug Dev Technol. 2010 Oct;8(5):625-9. doi: 
            10.1089/adt.2009.0266. Epub 2010 Jun 1.
  PUBMED    20515414
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/adt.2009.0266"; journalName: "Assay Drug Dev Technol"; 
            date: "2010-10"; volume: "8"; issue: "5"; pages: "625-9"
COMMENT     Sequence Label: pMBD-Gate6
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(105..111,112..129,130..135))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(160..284)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    370..491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(381..400)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     435..454
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(764..783)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     974..995
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga
      121 agcataaagt gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt
      181 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      241 atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgtttgata tcatctagag
      301 cggccgcagg tacctgaata actaaggccg cttcccttta gtgagggtta atgcttcgag
      361 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
      421 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
      481 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt
      541 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatccga taaggatcga
      601 tccgggctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      661 cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
      721 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      781 ccttcctttc acgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      841 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
      901 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
      961 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tat
//
