LOCUS       sequence-67080-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM
  TITLE     Linear DNA for rapid prototyping of synthetic biological circuits in
            an Escherichia coli based TX-TL cell-free system.
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. 
            Epub 2013 Dec 4.
  PUBMED    24303785
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400131a";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "387-97"
COMMENT     Sequence Label: pBEST-p15a-PLtetO-1-UTR1-LacI-T500
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..44
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    51..69
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(340..358)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        426..530
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(749..768)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaattattgc tagctgtgct cagtatctct atcactgata gggatgtcaa tctctatcac
       61 tgatagggag catgcccagc ggaacagcac acacatgtcg cggtggttac gtccgtcacg
      121 ttcacgcatc aggcggatat cgttagccca cccagcaaaa ttcggttttc tggctgatgg
      181 tgcgatagtc ttcaccatgt caaacatcca ctctgcggcg gtcaggtctt ctgctgtccc
      241 ccacttgctg gcgctctgaa ttgcagcatc cggtttcacc acagaaaggt cgttttctgg
      301 ctggtcagag gattcgccag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
      361 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      421 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      481 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      541 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      601 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      661 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
      721 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
      781 ccgggcaa
//
