LOCUS       sequence-67081-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  TITLE     Murray Lab pBEST plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBEST-p15a-pBAD-UTR1-TetR-T500
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             32..655
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(105..124)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgtctaga ttagataaaa gtaaagtgat
       61 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
      121 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
      181 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
      241 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
      301 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
      361 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
      421 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
      481 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
      541 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
      601 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt cttaactcga
      661 gcaaagcccg ccgaaaggcg ggctt
//
