LOCUS       sequence-67082-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  TITLE     Murray Lab pBEST plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBEST-p15a-pBAD-UTR1-TetR-T500
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             47..69
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(105..124)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(601..619)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        687..791
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgcgctgtt aatcacttta cttttatcta atctagacat ggtatatctc cttcttaaag
       61 ttaaacaaaa ttattgctag ccaggtagga tccgctaatc ttatggataa aaatgctatg
      121 gcatagcaaa gtgtgacgcc gtgcaaataa tcaatgtgga cttttctgcc gtgattatag
      181 acacttttgt tacgcgtttt tgtcatggct ttggtcccgc tttgttacag aatgctttta
      241 ataagcgggg ttaccggttt ggttagcgag aagagccagt aaaagacgca gtgacggcaa
      301 tgtctgatgc aatatggaca attggtttct gcatgcccag cggaacagca cacacatgtc
      361 gcggtggtta cgtccgtcac gttcacgcat caggcggata tcgttagccc acccagcaaa
      421 attcggtttt ctggctgatg gtgcgatagt cttcaccatg tcaaacatcc actctgcggc
      481 ggtcaggtct tctgctgtcc cccacttgct ggcgctctga attgcagcat ccggtttcac
      541 cacagaaagg tcgttttctg gctggtcaga ggattcgcca gaattcttga agacgaaagg
      601 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      661 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      721 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      781 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcc
//
