LOCUS       sequence-67084-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  TITLE     Murray Lab pBEST plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBEST-PLtetO-1-UTR1-TetR-deGFP-T500
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             47..69
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    93..111
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    118..136
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(380..398)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        466..570
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcgctgttaa tcactttact tttatctaat ctagacatgt ggtatatctc cttcttaaag
       61 ttaaacaaaa ttattgctag ctgtgctcag tatctctatc actgataggg atgtcaatct
      121 ctatcactga tagggagcat gcccagcgga acagcacaca catgtcgcgg tggttacgtc
      181 cgtcacgttc acgcatcagg cggatatcgt tagctgatgg tgcgatagtc ttcaccatgt
      241 caaacatcca ctctgcggcg gtcaggtctt ctgctgtccc ccacttgctg gcgctctgaa
      301 ttgcagcatc cggtttcacc acagaaaggt cgttttctgg ctggtcagag gattcgccag
      361 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
      421 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      481 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      541 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      601 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      661 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      721 agcggtaaga tcc
//
