LOCUS       sequence-67085-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  TITLE     Murray Lab pBEST plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBEST-PLlacO-1-UTR1-deCFP-T500
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    54..70
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(361..379)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        447..551
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtatatctcc ttcttaaagt taaacaaaat tattgctagc tgtgctcagc atcttgttat
       61 ccgctcacaa tgtcaatgtt atccgctcac atttatgcag cggaacagca cacacatgtc
      121 gcggtggtta cgtccgtcac gttcacgcat caggcggata tcgttagccc acccagcaaa
      181 attcggtttt ctggctgatg gtgcgatagt cttcaccatg tcaaacatcc actctgcggc
      241 ggtcaggtct tctgctgtcc cccacttgct ggcgctctga attgcagcat ccggtttcac
      301 cacagaaagg tcgttttctg gctggtcaga ggattcgcca gaattcttga agacgaaagg
      361 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      421 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      481 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      541 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      601 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      661 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      721 gttttcgc
//
