LOCUS       sequence-67087-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  TITLE     Murray Lab pBEST plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBEST-pTar-UTR1-TetR-T500
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             47..69
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(436..454)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        522..626
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgcgctgtt aatcacttta cttttatcta atcttgacat ggtatatctc cttcttaaag
       61 ttaaacaaaa ttattgctag cttgatctcg ttatcggcaa ggagggggga aactttattg
      121 aagcttggcc gggctcagca cgttaccgga ccagaagttg tcctggcatg cccagcggaa
      181 cagcacacac atgtcgcggt ggttacgtcc gtcacgttca cgcatcaggc ggatatcgtt
      241 agcccaccca gcaaaattcg gttttctggc tgatggtgcg atagtcttca ccatgtcaaa
      301 catccactct gcggcggtca ggtcttctgc tgtcccccac ttgctggcgc tctgaattgc
      361 agcatccggt ttcaccacag aaaggtcgtt ttctggctgg tcagaggatt cgccagaatt
      421 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
      481 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      541 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      601 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      661 ccttttttgc ggcattttgc cttcc
//
