LOCUS       sequence-67119-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   DiCostanzo AC, Thompson JR, Peterson FC, Volkman BF, 
            Ramirez-Alvarado M
  TITLE     Tyrosine residues mediate fibril formation in a dynamic light chain 
            dimer interface.
  JOURNAL   J Biol Chem. 2012 Aug 10;287(33):27997-8006. doi: 
            10.1074/jbc.M112.362921. Epub 2012 Jun 27.
  PUBMED    22740699
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.362921"; journalName: "J Biol Chem"; date: 
            "2012-08-10- 10"; volume: "287"; issue: "33"; pages: "27997-8006"
COMMENT     Sequence Label: pET12a-AL-103 Y32F
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(448..466)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      462..509
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(875..880,881..897,898..903))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 tatgcgggcg aaactcctag gaatagtcct gacaacccct atcgcgatca gctcttttgc
       61 gtcgacggac atccagatga cccagtctcc atcctccctg tctgcatctg taggagacag
      121 agtcaccatc acttgccagg cgagtcagga cattagtaat tttttaattt ggtatcaaca
      181 gaaaccaggg aaagccccta aactcctgat ctacgatgca tccaatttgg aaacaggggt
      241 cccatcaagg ttcagtggaa gtggatctgg gacagatttt actttcacca tcagcagcct
      301 gcagcctgaa gatattgcaa catattactg tcaacagtat cataatctcc ctccgtacac
      361 ttttggcccg gggaccaagc tggagatcaa acgataagga tccggctgct aacaaagccc
      421 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
      481 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac
      541 aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc
      601 aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat
      661 tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg
      721 acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc
      781 agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga
      841 ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt
      901 caaacatgag aattcttga
//
