LOCUS       sequence-67123-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     Ramirez Alvarado plasmids
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET12a-AL-103 delProIns
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(417..435)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      431..478
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(843..848,849..865,866..871))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ctgacaaccc ctatcgcgat cagctctttt gcgtcgacgg acatccagat gacccagtct
       61 ccatcctccc tgtctgcatc tgtaggagac agagtcacca tcacttgcca ggcgagtcag
      121 gacattagta attatttaat ttggtatcaa cagaaaccag ggaaagcccc taaactcctg
      181 atctacgatg catccaattt ggaaacaggg gtcccatcaa ggttcagtgg aagtggatct
      241 gggacagatt ttactttcac catcagcagc ctgcagcctg aagatattgc aacatattac
      301 tgtcaacagt atcataatct cccttacact tttggcccgg ggaccaagct ggagatcaaa
      361 cgataaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
      421 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      481 gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt
      541 cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca
      601 gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc
      661 catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg
      721 gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg
      781 cattgttaga tttcatacac ggtgcctgac tgcgttagca attaactgtg ataaactacc
      841 gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga c
//
