LOCUS       sequence-67124-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  TITLE     Ramirez Alvarado plasmids
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET12a-DAH
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(482..500)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      496..543
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(909..914,915..931,932..937))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcgggc gaaactccta ggaatagtcc
       61 tgacaacccc tatcgcgatc agctcttttg cgtcgacgca gtctgtgttg acgcagccgc
      121 cctcagtgtc tgcggcccca ggacagacgg tcaccatctc ctgctctgga agcagctcca
      181 acattgggaa taattatgta tcttggtacc agcacctccc aggaacagcc cccaaattcc
      241 tcatttatga caataataag cgaccctcag ggattcctga ccgattctct ggcttcaagt
      301 ctggcacgtc agccaccctg ggcatcaccg gactccagac tggggacgag gccgattatt
      361 actgcggaac atgggatagt agcctgagtg ctctggtctt cggcggaggg accaagctga
      421 ccgtcctata aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      481 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      541 ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc
      601 gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc
      661 ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag
      721 cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag
      781 taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat
      841 gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa
      901 actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct
//
