LOCUS       sequence-67125-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     Ramirez Alvarado plasmids
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET12a-HAL
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(472..490)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      486..533
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgcgggcg aaactcctag gaatagtcct
       61 gacaacccct atcgcgatca gctcttttgc gtcgacggac atccagatga cccagtctcc
      121 atcttccctg tctgcatctg taagagacag agtcaccatc acttgccggg caagtcagac
      181 cattagcaac tttttaaatt ggtatcagca gaaaccaggt aaagccccta agctcctggt
      241 ctatgctgca accagtttgc aaactggggt cccctcaggt ttcagtggca atggatcggg
      301 gacagagttc actctcacca tcagcagtct ccaacctgag gattttgcaa gttactactg
      361 tcaacagagt tacagtaccc cgtggacatt tggccaaggg accaaggtgg aaatcaaata
      421 aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      481 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      541 gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata
      601 gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct
      661 ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag
      721 tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata
      781 accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg
//
