LOCUS       sequence-67126-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  TITLE     Ramirez Alvarado plasmids
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET12a-JOH1
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(475..493)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      489..536
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgcgg gcgaaactcc taggaatagt
       61 cctgacaacc cctatcgcga tcagctcttt tgcgtcgacg gacatccaga tgacccagtc
      121 tccatcctca gtgtctgcat atgttggcga cagagtcacc atcacttgtc gggcgagtca
      181 gggcattaat atccgtttag cctggtttca gcagaagcca gggaaagccc ctgagtctct
      241 gatttatggt gcttccactt tgcaaagtgg tgtcccatca aagttcagcg gcagtggttc
      301 tgggacagat ttcactctca ccatcagcag cctgcggcct gaagattctg caacttatta
      361 ctgtcaacaa tataaaagtt ttccgctcac cttcggccaa gggacgcggc tggagataaa
      421 ataaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      481 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      541 aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg
      601 atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt
      661 gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca
      721 tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc
      781 ataacc
//
