LOCUS       sequence-67127-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  TITLE     Ramirez Alvarado plasmids
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET12a-KIo18/08 C214S
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(763..781)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      777..824
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tatgcgggcg aaactcctag gaatagtcct gacaacccct atcgcgatca gctcttttgc
       61 gtcgacggac atccagatga cccagtctcc atcctccctg tctgcatctg taggcgacag
      121 agtcaccatc acttgccagg cgagtcagga cattagcaac tatttaaatt ggtatcagca
      181 gaagccaggg aaagccccta agctcctgat ctacgatgca tccaatttgg aaacaggggt
      241 cccatcaagg ttcagtggaa gtggatctgg gacagatttc actttcacca tcagcagcct
      301 gcagcctgag gatattgcaa catattactg tcaacagtat gataatctcc cgtacacttt
      361 tggccagggg accaagctgg agatcaaacg aactgtggct gcaccatctg tcttcatctt
      421 cccgccatct gatgagcagt tgaaatctgg aactgcctct gttgtgtgcc tgctgaataa
      481 cttctatccc agagaggcca aagtacagtg gaaggtggat aacgccctcc aatcgggtaa
      541 ctcccaggag agtgtcacag agcaggacag caaggacagc acctacagcc tcagcagcac
      601 cctgacgctg agcaaagcag actacgagaa acacaaagtc tacgcctgcg aagtcaccca
      661 tcagggcctg agctcgcccg tcacaaagag cttcaacagg ggagagagtt aaggatccgg
      721 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
      781 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
      841 tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca
      901 agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg
      961 ggtgcc
//
