LOCUS       sequence-67137-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: AB.pCCL.sin.cPPT.U6.miR-489-Decoy.hPGK.GFP.WPRE
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..229
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_feature    454..571
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccgcgccggc cccctcgcac agacttgtgg gagaagctcg gctactcccc tgccccggtt
       61 aatttgcata taatatttcc tagtaactat agaggcttaa tgtgcgataa aagacagata
      121 atctgttctt tttaatacta gctacatttt acatgatagg cttggatttc tataagagat
      181 acaaatacta aattattatt ttaaaaaaca gcacaaaagg aaactcaccc taactgtaaa
      241 gtaattgtgt gttttgagac tataaatatc ccttggagaa aagccttgtt tgacggcgct
      301 aggatcatca acgctgccat atatcaggtg tcattcaagt attctggtca cagaatacaa
      361 cgctgccata tatcaggtgt cattcaagat gatcctagcg ccgtcttttt tgaattcgat
      421 atcaagcttc tcgaggctag tctcgtgatc gataaaattt tgaatttttg taatttgttt
      481 ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact
      541 gtacccccca atcccccctt ttcttttaaa agttaaccga taccgtcgag atccgttcac
      601 taatcgaatg gatctgtctc tgtctctctc tccaccttct tcttctattc cttcgggcct
      661 gtcgggtccc ctcggggttg ggaggtgggt ctgaaac
//
