LOCUS       sequence-67141-e         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: AB.pCCL.sin.cPPT.U6.miR-574-3p-Decoy.hPGK.GFP....
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     208..228
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_feature    451..568
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgcgccggcc ccctcgcaca gacttgtggg agaagctcgg ctactcccct gccccggtta
       61 atttgcatat aatatttcct agtaactata gaggcttaat gtgcgataaa agacagataa
      121 tctgttcttt ttaatactag ctacatttta catgataggc ttggatttct ataagagata
      181 caaatactaa attattattt taaaaaacag cacaaaagga aactcaccct aactgtaaag
      241 taattgtgtg ttttgagact ataaatatcc cttggagaaa agccttgttt gacggcgcta
      301 ggatcatcaa ctgtgggtgt gtcgatgagc gtgcaagtat tctggtcaca gaatacaact
      361 gtgggtgtgt cgatgagcgt gcaagatgat cctagcgccg tcttttttga attcgatatc
      421 aagcttctcg aggctagtct cgtgatcgat aaaattttga atttttgtaa tttgtttttg
      481 taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta
      541 ccccccaatc cccccttttc ttttaaaagt taaccgatac cgtcgagatc cgttcactaa
      601 tcgaatggat ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc
      661 gggtcccctc ggggttggga ggtgggtctg aaac
//
