LOCUS       sequence-67144-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Sun J, Lau A, Wang X, Liao TY, Zoubeidi A, Hmama Z
  TITLE     A broad-range of recombination cloning vectors in mycobacteria.
  JOURNAL   Plasmid. 2009 Nov . 62(3):158-65.
  PUBMED    19647016
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid. 
            2009 Nov . 62(3):158-65."
COMMENT     Sequence Label: pJAK1.A
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(101..107,108..125,126..131))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(156..280)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
       61 gctcctgaaa atctcgacgg atcctaactc aaaatccaca cattatacga gccggaagca
      121 taaagtgtaa agcctggggt gcctaatgcg gccgccatag tgactggata tgttgtgttt
      181 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
      241 cattttacgt ttctcgttca gcttttttgt acaaacttgt tgatctggat ccgcaattgt
      301 cttggccatt gcgaagtgat tcctccggat cggggatgaa acgggggtca ccgggtgacg
      361 gcaaccccgc tacttacgct ggccgggcgc agtgcccgcg acggacggct caagttgtcc
      421 tcgctgccac tcgctgcgac gacgggcctg gcctcaccgt cccgacctag cactcaccgg
      481 tcgcgagtgc caacgttatt cttagcactc gcctatgccg agtgcaagaa gccccgcacc
      541 gggtcatgcc cctcgttcga ccttgtcctc ggccctccga tccgggtgag tatgttcggc
      601 ccatgaccgc caacgacaac aagacccgta aatggtcggc cgcagacgtc cccgatcaaa
      661 gcgggcgcgt tgtggtcacc gtctagattt ctggcgggaa cggcaatgct cctggcggcg
      721 gcggggctgg tgcccaggtc tccgcacaga gcggcggtgc tggcgctcgc ggccaggcgt
      781 ggttcttcgc gtactgacaa gaaacccccc tctttaggac tcagtgtcct tgggaggggg
      841 gctttttgcg tttcaggagg tcttggccag cttggacatc
//
