LOCUS       sequence-67159-e        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Yasuda R, Harvey CD, Zhong H, Sobczyk A, van Aelst L, Svoboda K
  TITLE     Supersensitive Ras activation in dendrites and spines revealed by 
            two-photon fluorescence lifetime imaging.
  JOURNAL   Nat Neurosci. 2006 Feb . 9(2):283-91.
  PUBMED    16429133
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2006 Feb . 9(2):283-91."
COMMENT     Sequence Label: mEGFP-HRas
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..608
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=H-Ras
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     polyA_signal    632..753
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(643..662)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     697..716
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1021..1040)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagtccg gactcgagat gacggaatat aagctggtgg
       61 tggtgggcgc cggcggtgtg ggcaagagtg cgctgaccat ccagctgatc cagaaccatt
      121 ttgtggacga atacgacccc actatagagg attcctaccg gaagcaggtg gtcattgatg
      181 gggagacgtg cctgttggac atcctggata ccgccggcca ggaggagtac agcgccatgc
      241 gggaccagta catgcgcacc ggggagggct tcctgtgtgt gtttgccatc aacaacacca
      301 agtcttttga ggacatccac cagtacaggg agcagatcaa acgggtgaag gactcggatg
      361 acgtgcccat ggtgctggtg gggaacaagt gtgacctggc tgcacgcact gtggaatctc
      421 ggcaggctca ggacctcgcc cgaagctacg gcatccccta catcgagacc tcggccaaga
      481 cccggcaggg agtggaggat gccttctaca cgttggtgcg tgagatccgg cagcacaagc
      541 tgcggaagct gaaccctcct gatgagagtg gccccggctg catgagctgc aagtgtgtgc
      601 tctcctgagc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
      661 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
      721 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
      781 tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
      841 ggtaaaatcg ataaggatcc gggctggcgt aatagcgaag aggcccgcac cgatcgccct
      901 tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg
      961 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     1021 ctcctttcgc tttcttccct tcctttctcg c
//
