LOCUS       sequence-6716-ex        6815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Malagon F, Tong AH, Shafer BK, Strathern JN
  TITLE     Genetic interactions of DST1 in Saccharomyces cerevisiae suggest a 
            role of TFIIS in the initiation-elongation transition.
  JOURNAL   Genetics 2004 Mar;166(3):1215-27.
  PUBMED    15082542
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "2004-03"; volume: "166(3)"; pages: "1215-27"
COMMENT     Sequence Label: YEplac181-SOH1
FEATURES             Location/Qualifiers
     source          1..6815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1364..1381)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1364..1380)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1373..1395)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1817..3163
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        complement(4405..4803)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(4823..4841)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4909..5013
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5014..5082,5083..5874)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5232..5251)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6045..6633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6534..6553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6787..6804
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gaccacactg tcagtatggc ataccttttg aatgactgga
      301 caatgtgtct tcggtctctg ccggttgccc ccggcaccgc acggtagcgt ataatacacc
      361 accatccgac caaggaagtc caaaaccacc ctttctttac caacatagca tttaccagac
      421 catccttatt cagggttgct tgcagcgccg caccgtagcc atacgcgttc atgagatatc
      481 ctaccagtac cgtcaacggg cacaccagta ggtgtatagc attaaaccat ttgctagaca
      541 ttgttgtaat atgccgtgct ttatagctgg tttcagtgga gttttaggcg tttccccttt
      601 ggcttttttc gaacagttcg ttttgtattt tatttcacgg ttttcccgct aacgtgttct
      661 taaaggatga aaaagaaaaa gaaatgcagg aaaaatgagg caaagtaagc caaaacttat
      721 agcactcata agacggcaaa gttcccccaa gctccaccca ggatgtcaag cactaatgga
      781 aacgcaccag cgacaccttc ctcggatcaa aatcctctcc ccacgaggtt cgaagtggag
      841 ctcgaattta tccagtcact ggcaaacatc caatacgtaa cgtacttact cactcaacag
      901 cagatctgga agtctcctaa cttcaaaaac tacttgaaat acctggagta ctggtgcaac
      961 cccccttatt cgcaatgcat tgtgtatcca aactgtctct tcattttaaa gctgttgaac
     1021 gggttcatgg agtcggcgat cgttaatgaa gatggcctgc tggaagggct agacgagctg
     1081 cccaagatca ttcaattaca aggcccgcaa tggatgaatg aaatggtaga aagatgggcc
     1141 aactgaacac acctaattat aactacattc gcacgcattc atatatctat tttatacact
     1201 catgaatata tatacacaca cacacatata tatatcacat atatacatat atatatgtat
     1261 atatatatgc aagccgcatt actttgtatt ttctttcctt tgttttcctt gcatattatc
     1321 tcgcaagaca ttagaagatc tcgagggtac cgagctcgaa ttcactggcc gtcgttttac
     1381 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     1441 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     1501 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     1561 tttcacaccg catatatcgg atcgtacttg ttacccatca ttgaattttg aacatccgaa
     1621 cctgggagtt ttccctgaaa cagatagtat atttgaacct gtataataat atatagtcta
     1681 gcgctttacg gaagacaatg tatgtatttc ggttcctgga gaaactattg catctattgc
     1741 ataggtaatc ttgcacgtcg catccccggt tcattttctg cgtttccatc ttgcacttca
     1801 atagcatatc tttgttaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
     1861 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     1921 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
     1981 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     2041 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
     2101 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
     2161 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
     2221 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
     2281 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
     2341 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
     2401 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
     2461 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
     2521 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
     2581 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
     2641 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
     2701 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt
     2761 tggttttcaa aagcgctctg aagttcctat actttctagc tagagaatag gaacttcgga
     2821 ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc
     2881 acatacagct cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat atatatatac
     2941 atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg tacttatatg cgtctattta
     3001 tgtaggatga aaggtagtct agtacctcct gtgatattat cccattccat gcggggtatc
     3061 gtatgcttcc ttcagcacta ccctttagct gttctatatg ctgccactcc tcaattggat
     3121 tagtctcatc cttcaatgct atcatttcct ttgatattgg atcgatccga tgataagctg
     3181 tcaaacatga gaattaattc taccctatga acatattcca ttttgtaatt tcgtgtcgtt
     3241 tctattatga atttcattta taaagtttat gtacaaatat cataaaaaaa gagaatcttt
     3301 ttaagcaagg attttcttaa cttcttcggc gacagcatca ccgacttcgg tggtactgtt
     3361 ggaaccacct aaatcaccag ttctgatacc tgcatccaaa acctttttaa ctgcatcttc
     3421 aatggcctta ccttcttcag gcaagttcaa tgacaatttc aacatcattg cagcagacaa
     3481 gatagtggcg atagggtcaa ccttattctt tggcaaatct ggagcagaac cgtggcatgg
     3541 ttcgtacaaa ccaaatgcgg tgttcttgtc tggcaaagag gccaaggacg cagatggcaa
     3601 caaacccaag gaacctggga taacggaggc ttcatcggag atgatatcac caaacatgtt
     3661 gctggtgatt ataataccat ttaggtgggt tgggttctta actaggatca tggcggcaga
     3721 atcaatcaat tgatgttgaa ccttcaatgt aggaaattcg ttcttgatgg tttcctccac
     3781 agtttttctc cataatcttg aagaggccaa aacattagct ttatccaagg accaaatagg
     3841 caatggtggc tcatgttgta gggccatgaa agcggccatt cttgtgattc tttgcacttc
     3901 tggaacggtg tattgttcac tatcccaagc gacaccatca ccatcgtctt cctttctctt
     3961 accaaagtaa atacctccca ctaattctct gacaacaacg aagtcagtac ctttagcaaa
     4021 ttgtggcttg attggagata agtctaaaag agagtcggat gcaaagttac atggtcttaa
     4081 gttggcgtac aattgaagtt ctttacggat ttttagtaaa ccttgttcag gtctaacact
     4141 acctgtaccc catttaggac cacccacagc acctaacaaa acggcatcaa ccttcttgga
     4201 ggcttccagc gcctcatctg gaagtgggac acctgtagca tcgatagcag caccaccaat
     4261 taaatgattt tcgaaatcga acttgacatt ggaacgaaca tcagaaatag ctttaagaac
     4321 cttaatggct tcggctgtga tcttgaccaa cgtggtcacc tggcaaaacg acgatcttct
     4381 taggggcaga cataggggca gacattagaa tgtatatcct tgaaatatat atatatattg
     4441 ctgaaatgta aaaggtaaga aaagttagaa taagacgatt gctaaccacc tattggaaaa
     4501 aacaataggt ccttaaataa tattgtcaac ttcaagtatt gtgatgcaag catttagtca
     4561 tgaacgcttc tctattctat atgaaaagcc ggttccggcc tctcaccttt cctttttctc
     4621 ccaatttttc agttgaaaaa ggtatatgcg cggcgacctc tgaaattaac aaaaaatttc
     4681 cagtcatcga atttgtctgt gcgatagcgc ccctgtgtgt tctcgttatg ttgaggaaaa
     4741 aaataatggt tgctaagaga ttcgaactct tgcatcttac gatacctgag tattcccaca
     4801 gttaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4861 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4921 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4981 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5041 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5101 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5161 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5221 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5281 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5341 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5401 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5461 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5521 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5581 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5641 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5701 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5761 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5821 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5881 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5941 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6001 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6061 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6121 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6181 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6241 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6301 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6361 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6421 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6481 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6541 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6601 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6661 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6721 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6781 gagcgcagcg agtcagtgag cgaggaagcg gaaga
//
