LOCUS       sequence-67162-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Shen MW, Fang F, Sandmeyer S, Da Silva NA
  TITLE     Development and characterization of a vector set with regulated 
            promoters for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):495-503. doi: 10.1002/yea.2930. Epub 2012 Nov
            19.
  PUBMED    23166051
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2930"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "495-503"
COMMENT     Sequence Label: pXP821
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    578..611
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctaacaaaac ggcatcagcc ttcttggagg cttccagcgc ctcatctgga agtgggacac
       61 ctgtagcttc gatagcagca ccaccaatta aatgattttc gaaatcgaac ttgacattgg
      121 aacgaacatc agaaatagct ttaagaacct taatggcttc ggctgtgatt tcttgaccaa
      181 cgtggtcacc tggcaaaacg acgatcttct taggggcaga cattagaatg gtatatcctt
      241 gaaatatata tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa gtaagacgat
      301 tgctaaccac ctattggaaa aacaataggt ccttaaataa tattgtcaac ttcaagtatt
      361 gtgatgcaag catttagtca tgaacgcttc tctattctat atgaaaagcc ggttccggcg
      421 ctctcacctt tcctttttct cccaattttt cagttgaaaa aggtatatgc gtcaggcgac
      481 ctctgaaatt aacaaaaaat ttccagtcat cgaatttgat tctgtgcgat agcgcccctg
      541 tgtgttctcg ttatgttgag gaaaaaaata atggttaata acttcgtata atgtatgcta
      601 tacgaagtta tcccgggtac cgagctcgaa ttcaacgaag catctgtgct tcattttgta
      661 gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
      721 acagaacaga aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt
      781 ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
      841 tttttacaga acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt
      901 ctttttgtc
//
