LOCUS       sequence-67163-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Shen MW, Fang F, Sandmeyer S, Da Silva NA
  TITLE     Development and characterization of a vector set with regulated 
            promoters for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):495-503. doi: 10.1002/yea.2930. Epub 2012 Nov
            19.
  PUBMED    23166051
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2930"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "495-503"
COMMENT     Sequence Label: pXP822
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(73..106)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gtattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat ttaattatat
       61 cagttattac ccataacttc gtatagcata cattatacga agttatcccg ggtaccgagc
      121 tcgaattcaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc
      181 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag
      241 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag
      301 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
      361 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc
      421 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct
      481 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc
      541 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact
      601 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct
      661 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca
      721 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa
      781 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt
      841 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa
      901 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag
      961 caaagagata cttttga
//
