LOCUS       sequence-67164-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Shen MW, Fang F, Sandmeyer S, Da Silva NA
  TITLE     Development and characterization of a vector set with regulated 
            promoters for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):495-503. doi: 10.1002/yea.2930. Epub 2012 Nov
            19.
  PUBMED    23166051
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2930"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "495-503"
COMMENT     Sequence Label: pXP831
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    572..605
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aaacggcatc agccttcttg gaggcttcca gcgcctcatc tggaagtggg acacctgtag
       61 cttcgatagc agcaccacca attaaatgat tttcgaaatc gaacttgaca ttggaacgaa
      121 catcagaaat agctttaaga accttaatgg cttcggctgt gatttcttga ccaacgtggt
      181 cacctggcaa aacgacgatc ttcttagggg cagacattag aatggtatat ccttgaaata
      241 tatatatata ttgctgaaat gtaaaaggta agaaaagtta gaaagtaaga cgattgctaa
      301 ccacctattg gaaaaacaat aggtccttaa ataatattgt caacttcaag tattgtgatg
      361 caagcattta gtcatgaacg cttctctatt ctatatgaaa agccggttcc ggcgctctca
      421 cctttccttt ttctcccaat ttttcagttg aaaaaggtat atgcgtcagg cgacctctga
      481 aattaacaaa aaatttccag tcatcgaatt tgattctgtg cgatagcgcc cctgtgtgtt
      541 ctcgttatgt tgaggaaaaa aataatggtt aataacttcg tataatgtat gctatacgaa
      601 gttatcccgg gtaccgagct cgaattcaac gaagcatctg tgcttcattt tgtagaacaa
      661 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
      721 cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa
      781 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
      841 cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt
      901 tgttctacaa aaatgcatcc cgagagcgct
//
