LOCUS       sequence-67166-e       11679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Ping open reading frame 1 (ORF1) and transposase from 
            rice to allow mPing movement. The vector contains ORF1, transposase,
            mPing element and hph for hygromycin selection. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11679)
  AUTHORS   Hancock CN, Zhang F, Floyd K, Richardson AO, Lafayette P, Tucker D, 
            Wessler SR, Parrott WA
  TITLE     The rice miniature inverted repeat transposable element mPing is an 
            effective insertional mutagen in soybean.
  JOURNAL   Plant Physiol. 2011 Oct;157(2):552-62. doi: 10.1104/pp.111.181206. 
            Epub 2011 Aug 15.
  PUBMED    21844309
REFERENCE   2  (bases 1 to 11679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.111.181206"; journalName: "Plant Physiol"; date: 
            "2011-10"; volume: "157"; issue: "2"; pages: "552-62"
COMMENT     Sequence Label: pPing
FEATURES             Location/Qualifiers
     source          1..11679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        38..142
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(143..211,212..1003)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(361..380)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(1045..1554)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1240..1261)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1448..1467
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      1665..2253
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2154..2173
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2314..2335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2350..2355,2356..2373,2374..2380)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2388..2404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2393..2415
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2412..2428
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2412..2428
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(2462..2714)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2746..3456)
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(2786..2806)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     3401..3429
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     promoter        complement(3999..4344)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     promoter        complement(8418..8763)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             9942..10967
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(11480..11497)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11480..11496)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11489..11511)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gttaactacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
       61 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      241 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      301 gatccttgag agttttcgcc ccgaagaacg ttctccaatg atgagcactt ttaaagttct
      361 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
      421 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      481 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      541 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      601 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
      661 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
      721 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
      781 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
      841 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
      901 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
      961 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1021 ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg
     1081 aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta
     1141 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     1201 aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     1261 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     1321 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta
     1381 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcg aacgtggcga
     1441 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     1501 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg
     1561 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1621 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1681 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1741 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1801 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1861 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1921 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1981 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2041 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2101 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2161 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2221 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2281 ttcctggcct tttgctggcc ttttgctcac atgtaatgtg agttagctca ctcattaggc
     2341 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     2401 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctacggg gatcaattcc
     2461 cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt
     2521 ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc
     2581 ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat
     2641 tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa
     2701 atgtttgaac gatcggggaa attcgagctc ttaaagctca tcatgtttgt atagttcatc
     2761 catgccatgt gtaatcccag cagctgttac aaactcaaga aggaccatgt ggtctctctt
     2821 ttcgttggga tctttcgaaa gggcagattg tgtggacagg taatggttgt ctggtaaaag
     2881 gacagggcca tcgccaattg gagtattttg ttgataatga tcagcgagtt gcacgccgcc
     2941 gtcttcgatg ttgtggcggg tcttgaagtt ggctttgatg ccgttctttt gcttgtcggc
     3001 catgatgtat acgttgtggg agttgtagtt gtattccaac ttgtggccga ggatgtttcc
     3061 gtcctccttg aaatcgattc ccttaagctc gatcctgttg acgagggtgt ctccctcaaa
     3121 cttgacttca gcacgtgtct tgtagttccc gtcgtccttg aagaagatgg tcctctcctg
     3181 cacgtatccc tcaggcatgg cgctcttgaa gaagtcgtgc cgcttcatat gatctgggta
     3241 tcttgaaaag cattgaacac cataagagaa agtagtgaca agtgttggcc atggaacagg
     3301 tagttttcca gtagtgcaaa taaatttaag ggtaagtttt ccgtatgttg catcaccttc
     3361 accctctcca ctgacagaaa atttgtgccc attaacatca ccatctaatt caacaagaat
     3421 tgggacaact ccagtgaaaa gttcttctcc tttactgaat tcggccgagg ataatgatag
     3481 gagaagtgaa aagatgagaa agagaaaaag attagtcttc attgttatat ctccttggat
     3541 cctctagatt aggccagtca caatggctag tgtcattgca cggctaccca aaatattata
     3601 ccatcttctc tcaaatgaaa tcttttatga aacaatcccc acagtggagg ggtttcactt
     3661 tgacgtttcc aagactaagc aaagcattta attgatacaa gttgctggga tcatttgtac
     3721 ccaaaatccg gcgcggcgcg ggagaatgcg gaggtcgcac ggcggaggcg gacgcaagag
     3781 atccggtgaa tgaaacgaat cggcctcaac gggggtttca ctctgttacc gaggacttgg
     3841 aaacgacgct gacgagtttc accaggatga aactctttcc ttctctctca tccccatttc
     3901 atgcaaataa tcatttttta ttcagtctta cccctattaa atgtgcatga cacaccagtg
     3961 aaacccccat tgtgactggc cttatctaga gtcccccgtg ttctctccaa atgaaatgaa
     4021 cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg
     4081 tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
     4141 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
     4201 tcaacgatgg cctttccttt atcgcaatga tggcatttgt aggagccacc ttccttttcc
     4261 actatcttca caataaagtg acagatagct gggcaatgga atccgaggag gtttccggat
     4321 attacccttt gttgaaaagt ctcaattgcc ctttggtctt ctgagactgt atctttgata
     4381 tttttggagt agacaagtgt gtcgtgctcc accatgttga cgaagatttt cttcttgtca
     4441 ttgagtcgta agagactctg tatgaactgt tcgccagtct ttacggcgag ttctgttagg
     4501 tcctctattt gaatctttga ctccatggcc tttgattcag tgggaactac ctttttagag
     4561 actccaatct ctattacttg ccttggtttg tgaagcaagc cttgaatcgt ccatactgga
     4621 atagtacttc tgatcttgag aaatatatct ttctctgtgt tcttgatgca gttagtcctg
     4681 aatcttttga ctgcatcttt aaccttcttg ggaaggtatt tgatttcctg gagattattg
     4741 ctcgggtaga tcgtcttgat gagacctgct gcgtaagcct ctctaaccat ctgtgggtta
     4801 gcattctttc tgaaattgaa aaggctaatc tggggacctg caggagtcaa aaaacacagc
     4861 aacttgtgca tttcacttct aaaaatcctc agtaccagca acattaacaa ataagataga
     4921 taactgcata caagcatcag gtgaccaaat taaacattca tcggatgcac acaagcatca
     4981 ggtgaccaaa ttaaacattc atcggatgca cacaagcatc aagtgtccaa aataaaacag
     5041 tgaaatgaaa ctttagtgat ctgatagaag caactgggtg gatcattctg cccctcaacc
     5101 ttcagtccgt aatgcgttca gaaacctagt tccttgtttc atccagatga atgtgcacca
     5161 ccaccaaact tattccatat atgttccacc aaatcattct tgagtcggcg atgagccaaa
     5221 cgatctctca tactagtatc cttttctaaa attctttcta atggaacatg ctggtcagga
     5281 gagaattctg gtgcctgaac cgttgatgaa ctagcaggct catttaaatc tagattttct
     5341 tcaataagtc gcgcttcctt ctcatcttca actatcatat tgtgaagtat gatgcaacct
     5401 aggacaacat cacggagtac acctcggtca tatagacggg ctggtcgttt taagatgcag
     5461 aatcgacgtt gtagaacacc aaatgctctc tcgatatcct ttcttgcccc ttcttgatgt
     5521 tgtgcataca acttctcctt ctcagtgata gggagtcgat atgacttagc aaataccttc
     5581 cattcagggt aaattccatc agcaagaaaa taaccttcgt tgtattgatt cccatttacc
     5641 atgtactgca ctctaggagc ttgtcctttc agctcattga taaacacagt agatcggctc
     5701 aaaacattga tatcattgtt agaacctgct actccaaaga acgcatgcca aatccaaaga
     5761 tcatgtgatg ccactgcctc aagaatcagc gttggcactt tttgatcacc acgagtgaac
     5821 tgacccttcc acgcagttgg gcacctttcc cattgccaat gcatacagtc aatgctaccg
     5881 aacataccag gaaaaccgcg tctctcacca agctcgagta gtcgttcagt atcttctaca
     5941 gtgggacgcc tgagatatct ttcaccaaat acttctctaa ttcctttcac aaaatttttc
     6001 atagcatcca tagcagtagt ctctccaatc ttcaaatact catctagttc atcagcacca
     6061 ctaccagtag ccaattggcg aattgctgca gtacactttt gtaatggact aagcccttgc
     6121 ctaccagcgg catctaccct ctgagtaaag taatctgacc actggcctaa tgcgtccaca
     6181 atacgtaaaa acagcggcct gtacatacga aatcttcggc gaaaaatatt ggaaggatat
     6241 agaggatttt cagagaaata atcattcacc aaattttgat gtgcttcctc tcgtggcctc
     6301 ttgatgtgct tccttggatg caaacgatgg tcagcagcct ctctttcaag atctccgatg
     6361 gaggactgca ataacaccat catttcatgg agaacatcat ccattatctc atccatgatc
     6421 tcatcctcag cgagaaactc gtccaacaag gacacaggaa tctgattttc atttccagac
     6481 atcttcacag cagagaaaga gagaaggtga acaaaggaag attggagaaa taatcattca
     6541 cagcagagaa ataatcattc acagcagtga aaaaagatag aaacttcaaa gaaaggcaga
     6601 tcacctcaat gttgttgaac taaacaggaa gagagaaggg ctgcgtctat gccaggaaga
     6661 gatgaggact gcgtccagga attagagaag agggctgtgt gtatgccagg aagagatgag
     6721 gactgtatct atatatatag aggagggctg catccaggaa tgtgaaacta tagtggtcag
     6781 ttttcacacc acagtagcat tcacaccaca atagtagtgg tcagttttca caccacaata
     6841 gtactatagt ggtcagtttt cacacttgtg aaactgccat taaatagtgc tgcacgcaca
     6901 tgttgtgcat catgcataca ttatctaatt ttccatgttt acacaattgt atataaattt
     6961 cagaattcat acatttcaga aaatagtggt cagtttataa aactcacctt aatccgcaaa
     7021 tagcttctcc tctaatttcc gtattgccct ctggtggcta gccatttgat cttcagacat
     7081 gttgcttgta tccttactta atagagtatt gtacacatcg aacatctttg cttccttctg
     7141 ctccttagcg gccaacaatg ttgcatttga tatctgaatc tgtgcttcgg tggccttttc
     7201 acgatccacc ctccggtctt cgtggacctt tataattttc tggacattgt cccctaggag
     7261 gacaatgcct tccataacat tttctttctt gcgctttcca ttgcgctcag actttgctgc
     7321 ttctctacca ataggacgtg actgctgttc tggaacagca tccatttcgc tcttgtcttt
     7381 ctctgattca aactgagcac accatttggg ctcatctttg agtatcttcc accaatggac
     7441 caacgcaaaa ggttttccaa acctgtttgc atacagcctc tgtgcctctt tctcaagcat
     7501 gtcgtcggag tatccgcttg tatgcatttg agtaaccgta ctccaatagt cattgaactc
     7561 agagatcgct gacttcaacc ttgaccagtg aaccttcagt tggttaattt ccctcctacg
     7621 ttttccattc cctttcttgt taaattcatc agtgacttcc ttccaaaatg tatcaccttt
     7681 cttatcattc ccatgaattg agtctttaga agcattcaac caagcactgg ccagtctctc
     7741 ttcctcttca tgagtccatc ttttctttgc tgccctactg tcatcattgt tgtcctcttc
     7801 atcaatcata atgtttgttg aagtgccttg gttcgtagct tggggtgttg gtgttccgta
     7861 ggcacttggt ggctgtggag atattgggtt gaaccccata ttgtgaccaa cgaaatgaaa
     7921 attttcacct tgctgttgtg gctgcggcgg acaattttgt tgaataaaat tgacaaaccc
     7981 tcctggagga taacccctga ccaaacatga atttgggtct tgttggtttc caaagaaaga
     8041 tgcaaagttt ggggaaatgg tcgatgttgg tccccatcct cctccttgtg gttgcggtgg
     8101 ccttgccgtc gatgtccccg aagcaagaga ggaggtcggc gcagccggtg acggattcgg
     8161 cgaatcgggg acgaaaatcg gcgaagattg cggtggtcgt ggtgggatgg ccgtcggcgc
     8221 agcaggggat gaagacgtcg cagcaggagg cggggtggcc gggacagaaa tcggcggcgg
     8281 cctcttcaat cctagttgct tgcccccgcg ctgtttgcct cctttcccgg ttgcacgccg
     8341 ccgggtttca gcagccggcg acggatccac ggccggcgac ggatccacgg ccggcgacgg
     8401 atccatggag tcccccgtgt tctctccaaa tgaaatgaac ttccttatat agaggaaggg
     8461 tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa
     8521 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt
     8581 gggggtccat ctttgggacc actgtcggca gaggcatctt caacgatggc ctttccttta
     8641 tcgcaatgat ggcatttgta ggagccacct tccttttcca ctatcttcac aataaagtga
     8701 cagatagctg ggcaatggaa tccgaggagg tttccggata ttaccctttg ttgaaaagtc
     8761 tcaattgccc tttggtcttc tgagactgta tctttgatat ttttggagta gacaagtgtg
     8821 tcgtgctcca ccatgttgac gaagattttc ttcttgtcat tgagtcgtaa gagactctgt
     8881 atgaactgtt cgccagtctt tacggcgagt tctgttaggt cctctatttg aatctttgac
     8941 tccatgaagc ttctgcagat atccgcggat cctaggctag tgggcagatc gatccaaagc
     9001 acatacttat cgatttaaat ttcatcgaag agattaatat cgaataatca tatacatact
     9061 ttaaatacat aacaaatttt aaatacatat atctggtata taattaattt tttaaagtca
     9121 tgaagtatgt atcaaataca catatggaaa aaattaacta ttcataattt aaaaaataga
     9181 aaagatacat ctagtgaaat taggtgcatg tatcaaatac attaggaaaa gggcatatat
     9241 cttgatctag ataattaacg attttgattt atgtataatt tccaaatgaa ggtttatatc
     9301 tacttcagaa ataacaatat acttttatca gaacattcaa caaagcaaca accaactaga
     9361 gtgaaaaata cacattgttc tctagacata caaaattgag aaaagaatct caaaatttag
     9421 agaaacaaat ctgaatttct agaagaaaaa aataattatg cactttgcta ttgctcgaaa
     9481 aataaatgaa agaaattaga cttttttaaa agatgttaga ctagatatac tcaaaagcta
     9541 ttaaaggagt aatattcttc ttacattaag tattttagtt acagtcctgt aattaaagac
     9601 acattttaga ttgtatctaa acttaaatgt atctagaata catatatttg aatgcatcat
     9661 atacatgtat ccgacacacc aattctcata aaaaacgtaa tatcctaaac taatttatcc
     9721 ttcaagtcaa cttaagccca atatacattt tcatctctaa aggcccaagt ggcacaaaat
     9781 gtcaggccca attacgaaga aaagggcttg taaaacccta ataaagtggc actggcagag
     9841 cttacactct cattccatca acaaagaaac cctaaaagcc gcagcgccac tgatttctct
     9901 cctccaggcg aaggatctcg actctagggg ggcaataaga tatgaaaaag cctgaactca
     9961 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
    10021 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
    10081 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
    10141 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggcattc agcgagagcc
    10201 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
    10261 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
    10321 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
    10381 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
    10441 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
    10501 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
    10561 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
    10621 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
    10681 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
    10741 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
    10801 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
    10861 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
    10921 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagagt agatgccgac
    10981 cgggatcaat tcggcttgat ccaaattttg attttaatgt ttagcaaatg tcttatcagt
    11041 tttctctttt tgtcgaacgg taatttagag ttttttttgc tatatggatt ttcgtttttg
    11101 atgtatgtga caaccctcgg gattgttgat ttatttcaaa actaagagtt tttgtcttat
    11161 tgttctcgtc tattttggat atcaatctta gttttatatc ttttctagtt ctctacgtgt
    11221 taaatgttca acacactagc aatttggcct gccagcgtat ggattatgga actatcaagt
    11281 ctgtgggatc gataaatatg cttctcagga atttgagatt ttttatcatg tctttatgct
    11341 cattcccttg agtataatat agtaaaaaaa tagtaaattt aagcaataat gttaggtgct
    11401 atgtgtctgt cgagactatt gggggtagct taattaagcg taccctctta gtcaaggcct
    11461 taagtgagtc gtattacgga ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
    11521 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
    11581 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct
    11641 tcgcttggta ataaagcccg cttcggcggg ctttttttt
//
