LOCUS       sequence-67168-e        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Sarbassov DD, Guertin DA, Ali SM, Sabatini DM
  TITLE     Phosphorylation and regulation of Akt/PKB by the rictor-mTOR 
            complex.
  JOURNAL   Science 2005 Feb 18;307(5712):1098-101.
  PUBMED    15718470
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2005-02-18"; volume: "307(5712)"; pages: "1098-101"
COMMENT     Sequence Label: Rictor_1 shRNA
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     141..160
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    316..433
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        482..992
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     911..929
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(1014..1033)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa
       61 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
      121 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
      181 tcttggcttt atatatcttg tggaaaggac gaaacaccgg tacttgtgaa gaatcgtatc
      241 ttctcgagaa gatacgattc ttcacaagtt ttttgaattc tcgacctcga gacaaatggc
      301 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
      361 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
      421 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc cgcggctcga
      481 gggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
      541 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
      601 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
      661 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
      721 cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag
      781 cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca
      841 gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc
      901 tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc
      961 tcgttgaccg aatcaccgac ctctctcccc agggggatcc accggagctt accatgaccg
     1021 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     1081 tcgccgcc
//
