LOCUS       sequence-6717-ex        7535 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Malagon F, Tong AH, Shafer BK, Strathern JN
  TITLE     Genetic interactions of DST1 in Saccharomyces cerevisiae suggest a 
            role of TFIIS in the initiation-elongation transition.
  JOURNAL   Genetics 2004 Mar;166(3):1215-27.
  PUBMED    15082542
REFERENCE   2  (bases 1 to 7535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "2004-03"; volume: "166(3)"; pages: "1215-27"
COMMENT     Sequence Label: YEplac181-DST1
FEATURES             Location/Qualifiers
     source          1..7535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2084..2101)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2084..2100)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2093..2115)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2537..3883
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        complement(5125..5523)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(5543..5561)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5629..5733
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5734..5802,5803..6594)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5952..5971)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6765..7353
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7254..7273
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7507..7524
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgagatct taaattgtat ttctttagtt ctgaccgagc catatttaat
      301 gcatagaaga ttaataaaga aaacatgaaa atcataaata attaaaagta actggacgaa
      361 caaaagattt tacgtgagac aggacaaaaa aaattaacac cagcagtata tgtatatatg
      421 tacatcgtat atgaatatgc catttcaaca aattggatat ggttgtatgt ttggcttttt
      481 cttttcctgg attccggctg tcaaatacta ctacgaatag agtcagaaca ggcttttctg
      541 tcccttcctc ctattgtttc ctttgctctg tccgtggcta ctctcatctt tttctattct
      601 aagagaattt ccatctgtta ccacatgctt cacatgtaca gaaagtggtc aatggttcat
      661 ccgcagatct tgtttgcaat tgatagtaag ataccttctt ctctttacat ttaccacatg
      721 taaatctatc ggtgactgac ctttctatgg tggcaccctg tgcgttgtat aagttttgct
      781 tggcaatttc ttctatcttt tgctttaagg gcgctggtgc cagatccttg gcatcgcatg
      841 tagctaagaa ttcaggtgtt atatcaccgt tggcaatttt atgtttgaga tctgggttat
      901 tctttgatat gacgtttgaa taaattatac gatacctggc tttgtaagcg gcttcgttgg
      961 tgtcacagtt gttaacttta ttcatttcac tttctatggc ctttgcagta tgcaaaatag
     1021 attgaggtgg atgctcactt tccttggcca aaacgtcgta gagtgctttt agtacctgat
     1081 cacgtaattt gtggtggtat atagctgtat ccacaccatc gttcttacta tttcttggtt
     1141 tagtgctgac gtacttgtct tgtttcatgg catctgactg ggaggaggcc ggctgttgaa
     1201 caccattcac ggactcacct acagttgtct tgtcctctgc attgcctggc gcatgatctt
     1261 gatgatgctg ctgtgcttgc ctggaacgct tatttttatt aattgcgtct ttccaagagc
     1321 taatcatttt cttcacgagt ttgctgatct ctacattagt ggatttttta aacttgttga
     1381 cttccacacc aacttttgtt tctctcagta acttttcagt ggggacgaat tctttatcca
     1441 agacatgtaa gatttctaga actgcagcat cattactttt gttcttttct agattcttaa
     1501 catgtaccag tacttcctta ctatccatga tgaatgctct tatgcggact gactacactg
     1561 tgaatgttct gctgcttgag taatttttca ttcaatattt ttcaatacgt agtcccatca
     1621 tcgagtgatc acttggtcat atcagcacag tggggcgggt aagagttgaa aatttcccta
     1681 taatgaaatt ggatattata tatcaaaaag gcgttgtcat tcgaagcatc gtttcaaaag
     1741 aaggtccgta aagttaaggt gggctagtga agtatctcat aattgttaca atcaccataa
     1801 gagaaatctt gcgtttagaa tttcctaaga gaaccgagtc acttctagca cttgctttaa
     1861 acagaaacac ttgtaataat tgttagaggg atatacaaga aaggctatat accaatagca
     1921 tcacatagac acgttaatta tcaatacatt gaaagaaaag acgaaaacga taaaatgaat
     1981 aggcagagcg gtgtgaatgc gggggtacag aacaacccac catcccgagt ggtgtatctg
     2041 ggttctatac catacgggat ccccgggtac cgagctcgaa ttcactggcc gtcgttttac
     2101 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     2161 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     2221 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     2281 tttcacaccg catatatcgg atcgtacttg ttacccatca ttgaattttg aacatccgaa
     2341 cctgggagtt ttccctgaaa cagatagtat atttgaacct gtataataat atatagtcta
     2401 gcgctttacg gaagacaatg tatgtatttc ggttcctgga gaaactattg catctattgc
     2461 ataggtaatc ttgcacgtcg catccccggt tcattttctg cgtttccatc ttgcacttca
     2521 atagcatatc tttgttaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
     2581 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     2641 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
     2701 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     2761 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
     2821 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
     2881 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
     2941 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
     3001 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
     3061 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
     3121 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
     3181 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
     3241 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
     3301 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
     3361 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
     3421 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt
     3481 tggttttcaa aagcgctctg aagttcctat actttctagc tagagaatag gaacttcgga
     3541 ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc
     3601 acatacagct cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat atatatatac
     3661 atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg tacttatatg cgtctattta
     3721 tgtaggatga aaggtagtct agtacctcct gtgatattat cccattccat gcggggtatc
     3781 gtatgcttcc ttcagcacta ccctttagct gttctatatg ctgccactcc tcaattggat
     3841 tagtctcatc cttcaatgct atcatttcct ttgatattgg atcgatccga tgataagctg
     3901 tcaaacatga gaattaattc taccctatga acatattcca ttttgtaatt tcgtgtcgtt
     3961 tctattatga atttcattta taaagtttat gtacaaatat cataaaaaaa gagaatcttt
     4021 ttaagcaagg attttcttaa cttcttcggc gacagcatca ccgacttcgg tggtactgtt
     4081 ggaaccacct aaatcaccag ttctgatacc tgcatccaaa acctttttaa ctgcatcttc
     4141 aatggcctta ccttcttcag gcaagttcaa tgacaatttc aacatcattg cagcagacaa
     4201 gatagtggcg atagggtcaa ccttattctt tggcaaatct ggagcagaac cgtggcatgg
     4261 ttcgtacaaa ccaaatgcgg tgttcttgtc tggcaaagag gccaaggacg cagatggcaa
     4321 caaacccaag gaacctggga taacggaggc ttcatcggag atgatatcac caaacatgtt
     4381 gctggtgatt ataataccat ttaggtgggt tgggttctta actaggatca tggcggcaga
     4441 atcaatcaat tgatgttgaa ccttcaatgt aggaaattcg ttcttgatgg tttcctccac
     4501 agtttttctc cataatcttg aagaggccaa aacattagct ttatccaagg accaaatagg
     4561 caatggtggc tcatgttgta gggccatgaa agcggccatt cttgtgattc tttgcacttc
     4621 tggaacggtg tattgttcac tatcccaagc gacaccatca ccatcgtctt cctttctctt
     4681 accaaagtaa atacctccca ctaattctct gacaacaacg aagtcagtac ctttagcaaa
     4741 ttgtggcttg attggagata agtctaaaag agagtcggat gcaaagttac atggtcttaa
     4801 gttggcgtac aattgaagtt ctttacggat ttttagtaaa ccttgttcag gtctaacact
     4861 acctgtaccc catttaggac cacccacagc acctaacaaa acggcatcaa ccttcttgga
     4921 ggcttccagc gcctcatctg gaagtgggac acctgtagca tcgatagcag caccaccaat
     4981 taaatgattt tcgaaatcga acttgacatt ggaacgaaca tcagaaatag ctttaagaac
     5041 cttaatggct tcggctgtga tcttgaccaa cgtggtcacc tggcaaaacg acgatcttct
     5101 taggggcaga cataggggca gacattagaa tgtatatcct tgaaatatat atatatattg
     5161 ctgaaatgta aaaggtaaga aaagttagaa taagacgatt gctaaccacc tattggaaaa
     5221 aacaataggt ccttaaataa tattgtcaac ttcaagtatt gtgatgcaag catttagtca
     5281 tgaacgcttc tctattctat atgaaaagcc ggttccggcc tctcaccttt cctttttctc
     5341 ccaatttttc agttgaaaaa ggtatatgcg cggcgacctc tgaaattaac aaaaaatttc
     5401 cagtcatcga atttgtctgt gcgatagcgc ccctgtgtgt tctcgttatg ttgaggaaaa
     5461 aaataatggt tgctaagaga ttcgaactct tgcatcttac gatacctgag tattcccaca
     5521 gttaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5581 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5641 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5701 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5761 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5821 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5881 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5941 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     6001 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     6061 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     6121 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6181 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6241 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6301 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6361 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6421 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6481 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6541 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6601 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6661 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6721 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6781 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6841 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6901 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6961 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7021 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7081 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7141 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7201 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7261 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7321 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7381 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7441 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7501 gagcgcagcg agtcagtgag cgaggaagcg gaaga
//
