LOCUS       sequence-67170-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Zeng M, Kuzirian MS, Harper L, Paradis S, Nakayama T, Lau NC
  TITLE     Organic small hairpin RNAs (OshR): A do-it-yourself platform for 
            transgene-based gene silencing.
  JOURNAL   Methods. 2013 May 23. pii: S1046-2023(13)00148-5. doi: 
            10.1016/j.ymeth.2013.05.007.
  PUBMED    23707624
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2013 May 23. pii: S1046-2023(13)00148-5. doi: 
            10.1016/j.ymeth.2013.05.007."
COMMENT     Sequence Label: pGSH0
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..27
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9..27
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    130..264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(167..186)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     221..240
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(336..356)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 atgcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctccaccg cggtggcggc
       61 cgcgaattaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
      121 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
      181 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
      241 aatgtatctt atcatgtctg gatctacgta atacgactca ctatagttct agatttttgt
      301 atagttcatc catgccatgt gtaatcccag cagccgttac aaactcaaga aggaccatgt
      361 ggtctctctt ttcgttggga tctttcgaaa gggcagattg tgtggacagg taatggttgt
      421 ctggtaaaag gacagggtca tcgccaattg gagtattttg ttgataatga tcagcgagtt
      481 gcacgccgcc gtcttcgatg ttgtggcggg tcttgaagtt ggctttgatg ccgttctttt
      541 gcttgtcggc catgatgtat acgttgtggg agttgtagtt gtattccaac ttgtggccga
      601 ggatgtttcc gtcctccttg aaatcgattc ccttaagtag gatacacatt caatgttccc
      661 acattttatt ttggcacttt aactattagt cacataatat acaagtgatc agcaaaacag
      721 aaaaactctt cacatcaact cagtcccgaa agatggtatg ccatcatctc acttgtatta
      781 agggaagcta cgcgtgaaga tgcatgaagg tcgacgaagg catgcagctt tttattctac
      841 ctactcagat gtgaactttt cttaccttaa gctcgatcct gttgac
//
