LOCUS       sequence-67175-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Conway DE, Breckenridge MT, Hinde E, Gratton E, Chen CS, Schwartz MA
  TITLE     Fluid Shear Stress on Endothelial Cells Modulates Mechanical Tension
            across VE-Cadherin and PECAM-1.
  JOURNAL   Curr Biol. 2013 May 14. pii: S0960-9822(13)00490-9. doi: 
            10.1016/j.cub.2013.04.049.
  PUBMED    23684974
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2013 May 14. pii: S0960-9822(13)00490-9. doi: 
            10.1016/j.cub.2013.04.049."
COMMENT     Sequence Label: pLPCX-VEcadTS
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(454..705)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(496..517)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccctttttct ggagactaaa taaaatcttt tattttatcg atgtttggcc gaggcggccg
       61 cttgtcgaca ggccttaatg gcctaacgaa ttcctagatg atgagttcct cctggggatc
      121 tgagccgtac agctcagcca gcatcttgaa cctgggtccc cagtcgttga ggaagtcata
      181 atcgatgtca gagtcggagg aattggtgct cagggaactg agggactctg cgatggactc
      241 tgcgccctcg tagccgtaga tgtgcagtgt gtcgtatggg gggccaccgc cgtcattgtc
      301 tgcctcttct ttcttcacgt cgatcatggt ggccatctcc ctgggccctc cgtgcagtcc
      361 aggcgccagg cgcggcggct tctgcacctg cgtgtacact gccggccggg cgtccatcgt
      421 ggagcgcagg ggcttagtgg agcccgcggc cgccttgtac agctcgtcca tgccgagagt
      481 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
      541 tttgctcagt ttggactggt agctcaggta gtggttgtcg ggcagcagca cggggccgtc
      601 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgccgccgt cctcgatgtt
      661 gtggcggatc ttgaagttgg ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac
      721 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
      781 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
      841 gcgggtcttg ta
//
