LOCUS       sequence-67179-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Conway DE, Breckenridge MT, Hinde E, Gratton E, Chen CS, Schwartz MA
  TITLE     Fluid Shear Stress on Endothelial Cells Modulates Mechanical Tension
            across VE-Cadherin and PECAM-1.
  JOURNAL   Curr Biol. 2013 May 14. pii: S0960-9822(13)00490-9. doi: 
            10.1016/j.cub.2013.04.049.
  PUBMED    23684974
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2013 May 14. pii: S0960-9822(13)00490-9. doi: 
            10.1016/j.cub.2013.04.049."
COMMENT     Sequence Label: pLPCX-VEcadTL
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(463..714)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(505..526)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     854..873
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggtttcccct ttttctggag actaaataaa atcttttatt ttatcgatgt ttggccgagg
       61 cggccgcttg tcgacaggcc ttaatggcct aacgaattcc tagatgatga gttcctcctg
      121 gggatctgag ccgtacagct cagccagcat cttgaacctg ggtccccagt cgttgaggaa
      181 gtcataatcg atgtcagagt cggaggaatt ggtgctcagg gaactgaggg actctgcgat
      241 ggactctgcg ccctcgtagc cgtagatgtg cagtgtgtcg tatggggggc caccgccgtc
      301 attgtctgcc tcttctttct tcacgtcgat catggtggcc atctccctgg gccctccgtg
      361 cagtccaggc gccaggcgcg gcggcttctg cacctgcgtg tacactgccg gccgggcgtc
      421 catcgtggag cgcaggggct tagtggagcc cgcggccgcc tacttgtaca gctcgtccat
      481 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
      541 gttggggtct ttgctcagtt tggactggta gctcaggtag tggttgtcgg gcagcagcac
      601 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgccgccgtc
      661 ctcgatgttg tggcggatct tgaagttggc cttgatgccg ttcttctgct tgtcggcggt
      721 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
      781 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
      841 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctgga
//
