LOCUS       sequence-6718-ex        7358 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Malagon F, Tong AH, Shafer BK, Strathern JN
  TITLE     Genetic interactions of DST1 in Saccharomyces cerevisiae suggest a 
            role of TFIIS in the initiation-elongation transition.
  JOURNAL   Genetics 2004 Mar;166(3):1215-27.
  PUBMED    15082542
REFERENCE   2  (bases 1 to 7358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "2004-03"; volume: "166(3)"; pages: "1215-27"
COMMENT     Sequence Label: YEplac181-TAF14
FEATURES             Location/Qualifiers
     source          1..7358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1907..1924)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1907..1923)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1916..1938)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2360..3706
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        complement(4948..5346)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(5366..5384)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5452..5556
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5557..5625,5626..6417)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5775..5794)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6588..7176
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7077..7096
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7330..7347
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gaggatcccc aacttcgcat tgtttggttc cctaggtata
      301 ttcttttgac gtggtcaccc tatcttctag caacctggtc ccctcgtgtt tttcttttta
      361 ttcttttttc ttttccattt tctattacat tggaatgtat atatttgatg ttctcatttt
      421 ctaaaatgaa aactgttgta taattactta aaatccaaga taaaaatgtg ttgtaataat
      481 atgtgtttgg taaggtgtcg cggttattgg aataaagttt atataagaca aaatatcaac
      541 gtgttttatt tatacaaaca taaaagcgcg catttaacgc ccttttacct tttattactc
      601 ggtatttttc ttaacgtagt cccatagact tttcaataat ccctcaggta aactatacaa
      661 gtcaattata aattcaccct cttcaacatt attcgtcacg ttcatttctg gtgttttatt
      721 gtcggtaacc atttgaacaa caccaaccag gtcatcttca tttagtttag tcaatccgaa
      781 cgctaatttt tctaggtcca cgctcccttt cacggtagat gcactgcccg tcttagctct
      841 cttcgccttt ggttctgcag ttgtgttcgt ggtagtcctt cttttcccaa tggtgcctgt
      901 attggccgtc gtttcttcag tagatccact tttcgcaagt tcttctgtta aaagtggttt
      961 attgagagga atctggataa cgtgctccac ttcataactc tcttgtagaa agttcaaatc
     1021 gtgtggtatt ttccgctcac ctgctttttc caacaagaaa acgcttatat ctaaaggaaa
     1081 tccaccccaa ccttgttcct caattctaaa tggtgggtca gtaaatgtcc tgttaggatt
     1141 ggcgaaagtt gggtgcaagt ggtaaataac tttgtcaaaa attgtagcgg gaatttcttt
     1201 tccttcatca tccaataata caatttctat actccattga cgaacaggaa aattctctac
     1261 tggtggcact tctggcagaa tgtgttgttg ggtttttata cggatggttc tttttactgt
     1321 ctgttggtta tttaatgatg agaatagagt cgtaagttag tattatttta gctaacaaaa
     1381 caaaaagcat atgggtgggt ggctaaaaga gattaacgaa catacagcta ccatgattag
     1441 ttatctaata tcagtcagct ccgaggtcac gaaggagaaa aattacggta tattcttttt
     1501 ttttcaccgc actacagcct tgacgtgtaa tactgtaaca agttcaaact tcaagttttt
     1561 tgtcagtact ttgctaaaga gctttctcgt cttgagtgat tttaaaaagt gacagtaaat
     1621 aaaacaggaa agctagccaa caatatttct tatttaaata aaataatgat ctattaatac
     1681 gcctgtataa ataataaaat ggttaaaagt accgcaagag ccactgttgt aggagtcaaa
     1741 aagttgctcg cattatcgta gtcctcattc aatgatgaaa tattcgaata aggtgtagaa
     1801 gaagcaacgt aggagtgaga agtcacgaaa ggccgaatag tagtcgttga ttcagttgta
     1861 cttgcattga taaggtttcc atcgtaagta ttaacaatat gaattcactg gccgtcgttt
     1921 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     1981 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     2041 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     2101 gtatttcaca ccgcatatat cggatcgtac ttgttaccca tcattgaatt ttgaacatcc
     2161 gaacctggga gttttccctg aaacagatag tatatttgaa cctgtataat aatatatagt
     2221 ctagcgcttt acggaagaca atgtatgtat ttcggttcct ggagaaacta ttgcatctat
     2281 tgcataggta atcttgcacg tcgcatcccc ggttcatttt ctgcgtttcc atcttgcact
     2341 tcaatagcat atctttgtta acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa
     2401 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
     2461 caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa
     2521 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
     2581 aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac
     2641 aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc
     2701 ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt
     2761 tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc
     2821 ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc
     2881 gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg
     2941 atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta
     3001 cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac
     3061 tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag
     3121 tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca
     3181 gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt
     3241 ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc
     3301 ttttggtttt caaaagcgct ctgaagttcc tatactttct agctagagaa taggaacttc
     3361 ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg
     3421 cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata
     3481 tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat
     3541 ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt
     3601 atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg
     3661 gattagtctc atccttcaat gctatcattt cctttgatat tggatcgatc cgatgataag
     3721 ctgtcaaaca tgagaattaa ttctacccta tgaacatatt ccattttgta atttcgtgtc
     3781 gtttctatta tgaatttcat ttataaagtt tatgtacaaa tatcataaaa aaagagaatc
     3841 tttttaagca aggattttct taacttcttc ggcgacagca tcaccgactt cggtggtact
     3901 gttggaacca cctaaatcac cagttctgat acctgcatcc aaaacctttt taactgcatc
     3961 ttcaatggcc ttaccttctt caggcaagtt caatgacaat ttcaacatca ttgcagcaga
     4021 caagatagtg gcgatagggt caaccttatt ctttggcaaa tctggagcag aaccgtggca
     4081 tggttcgtac aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg acgcagatgg
     4141 caacaaaccc aaggaacctg ggataacgga ggcttcatcg gagatgatat caccaaacat
     4201 gttgctggtg attataatac catttaggtg ggttgggttc ttaactagga tcatggcggc
     4261 agaatcaatc aattgatgtt gaaccttcaa tgtaggaaat tcgttcttga tggtttcctc
     4321 cacagttttt ctccataatc ttgaagaggc caaaacatta gctttatcca aggaccaaat
     4381 aggcaatggt ggctcatgtt gtagggccat gaaagcggcc attcttgtga ttctttgcac
     4441 ttctggaacg gtgtattgtt cactatccca agcgacacca tcaccatcgt cttcctttct
     4501 cttaccaaag taaatacctc ccactaattc tctgacaaca acgaagtcag tacctttagc
     4561 aaattgtggc ttgattggag ataagtctaa aagagagtcg gatgcaaagt tacatggtct
     4621 taagttggcg tacaattgaa gttctttacg gatttttagt aaaccttgtt caggtctaac
     4681 actacctgta ccccatttag gaccacccac agcacctaac aaaacggcat caaccttctt
     4741 ggaggcttcc agcgcctcat ctggaagtgg gacacctgta gcatcgatag cagcaccacc
     4801 aattaaatga ttttcgaaat cgaacttgac attggaacga acatcagaaa tagctttaag
     4861 aaccttaatg gcttcggctg tgatcttgac caacgtggtc acctggcaaa acgacgatct
     4921 tcttaggggc agacataggg gcagacatta gaatgtatat ccttgaaata tatatatata
     4981 ttgctgaaat gtaaaaggta agaaaagtta gaataagacg attgctaacc acctattgga
     5041 aaaaacaata ggtccttaaa taatattgtc aacttcaagt attgtgatgc aagcatttag
     5101 tcatgaacgc ttctctattc tatatgaaaa gccggttccg gcctctcacc tttccttttt
     5161 ctcccaattt ttcagttgaa aaaggtatat gcgcggcgac ctctgaaatt aacaaaaaat
     5221 ttccagtcat cgaatttgtc tgtgcgatag cgcccctgtg tgttctcgtt atgttgagga
     5281 aaaaaataat ggttgctaag agattcgaac tcttgcatct tacgatacct gagtattccc
     5341 acagttaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     5401 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5461 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5521 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5581 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5641 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5701 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5761 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5821 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5881 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5941 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6001 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6061 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     6121 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6181 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6241 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6301 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6361 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6421 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6481 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6541 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6601 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6661 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6721 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6781 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6841 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6901 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6961 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7021 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7081 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7141 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7201 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7261 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7321 accgagcgca gcgagtcagt gagcgaggaa gcggaaga
//
