LOCUS       sequence-67180-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Arch voltage indicator with double mutations D95Q-D106E (Arch-EEQ), 
            with faster kinetics and greater fluorescence dynamic range than 
            Arch-D95N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Gong Y, Li JZ, Schnitzer MJ
  TITLE     Enhanced Archaerhodopsin Fluorescent Protein Voltage Indicators.
  JOURNAL   PLoS One. 2013 Jun 19;8(6):e66959.
  PUBMED    23840563
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-06-19"; volume: "8(6)"; pages: "e66959"
COMMENT     Sequence Label: pLenti-Arch-EEQ
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(76..786,787..789))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(118..139)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     467..486
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     726..747
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattctc attacacctc
       61 gttctcgtag cagaacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
      121 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
      181 gtagctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
      241 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
      301 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
      361 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
      421 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
      481 gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
      541 gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag cactgcaggc cgtagccgaa
      601 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag
      661 ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc
      721 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc
      781 cttgctcacc acgttgatgt cgatctggtc ca
//
