LOCUS       sequence-67181-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  TITLE     GSK3 plasmids
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GSK3 beta pGEX
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..68
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     53..69
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(628..639)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 cgaaacgcgc gaggcagatc gtcagtcagt cacgatgaat taagcttgag ctcgaggtcg
       61 acggtatcga taagcttgat atcgaattcc tttttttttt ttttttaata ttctttccaa
      121 acgtgaccag tgttgctgag tgacactcaa gtaactggtg gtttttcctg tgcagctggc
      181 tgctcgggac tgttcaggtg gagttggaag ctgatgcaga agcagcatta ttggtctgtc
      241 cacggtctcc agtattagca tctgacgctg ctgtggcatt tgtgggggtt gaagcagctg
      301 cttgaatccg agcatgagga ggaataagga tggtagccag aggtggatta cttgacagtt
      361 cttgagtggt gaagttgaag agtgcaggtg tgtctcgccc atttggtagt ttgacatttg
      421 ggtcccgtaa ttcatcaaaa aatgaatgtg cacaagcttc cagtggtgtt agtcgggcag
      481 ttggtgtata ctccagcaga cggctacaca gtgcaattgc ctccggtgga gttcggggtc
      541 ggaagacctt agtccaagga tgtgccttaa tttgagggaa tttaaattct gtgtagtttg
      601 ggttcatttc tctgatttgc tcccttgttg gagttcccag gaccttgatt atttctacca
      661 actgatccac accactatcc cctggaaata ttggttgtcc tagtaacagc tcagccaaca
      721 cacagccagc agaccataca tctatactag aggtataatc agtggctcca aagatcaact
      781 ctggtgccct atagtaccga gaacagatat acgaaacatt gggttctcct cggaccagct
      841 gctttgcact tccaaagtca cagagtttta atacagcagt atcaggatcc agcaagaggt
      901 tctgtggttt aatgtctcga tggc
//
