LOCUS       sequence-67186-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of a constitutively active form of K-Ras.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  TITLE     Viral vectors.
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBABE puro K-Ras V12
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(3..527,528..530,531..563))
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVI"
     primer_bind     complement(596..612)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(628..650)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ataattacac actttgtctt tgacttcttt ttcttctttt taccatcttt gctcatcttt
       61 tctttatgtt ttcgaatttc tcgaactaat gtatagaagg catcatcaac accctgtctt
      121 gtctttgctg atgtttcaat aaaaggaatt ccataacttc ttgctaagtc ctgagcctgt
      181 tttgtgtcta ctgttctgga aggcaaatca catttatttc ctactaggac cataggtaca
      241 tcttcagagt ccttaactct tttaatttgt tctctataat ggtgaatatc ttcaaatgat
      301 ttagtattat ttatggcaaa tacacaaaga aagccctccc cagtcctcat gtactggtcc
      361 ctcattgcac tgtactcctc ttgacctgct gtgtcgagaa tatccaagag acaggtttct
      421 ccatcaatta ctacttgctt cctgtaggaa tcctctattg ttggatcata ttcgtccaca
      481 aaatgattct gaattagctg tatcgtcaag gcactcttgc ctacgccaac agctccaact
      541 accacaagtt tatattcagt catggtggcg gatccggccg gcgcctagag aaggagtgag
      601 ggctggataa agggaggatc gaggcggggt cgaacgagga ggttcaaggg ggagagacgg
      661 ggcggatgga ggaagaggag gcggaggctt agggtgtaca aagggcttga cccagggagg
      721 ggggtcaaaa gccaaggctt cccaggtcac gatgtagggg acctggtctg ggtgtccatg
      781 cgggccaggt gaaaagacct tgatcttaac ctgggtgatg aggtctcggt taaaggtgcc
      841 gtctcgcggc catccgacgt taaaggttgg ccattctgca gagcagaagg taacccaacg
      901 tctcttcttg acatctaccg actggttgtg agcgatccgc tcga
//
