LOCUS       sequence-6719-ex        9112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Malagon F, Tong AH, Shafer BK, Strathern JN
  TITLE     Genetic interactions of DST1 in Saccharomyces cerevisiae suggest a 
            role of TFIIS in the initiation-elongation transition.
  JOURNAL   Genetics 2004 Mar;166(3):1215-27.
  PUBMED    15082542
REFERENCE   2  (bases 1 to 9112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "2004-03"; volume: "166(3)"; pages: "1215-27"
COMMENT     Sequence Label: YEplac181-RTS1
FEATURES             Location/Qualifiers
     source          1..9112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3661..3678)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3661..3677)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3670..3692)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4114..5460
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        complement(6702..7100)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(7120..7138)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7206..7310
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7311..7379,7380..8171)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7529..7548)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8342..8930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8831..8850
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9084..9101
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gaggatcccc tcgaagatat atttggagaa aagtatagaa
      301 ataataaaat gaagacacat cgattgaagt atgctcaaat gatgagaaaa agagaccttt
      361 atctcaggcc acaaacaacc attgtccaag tggcttatct ttgctatagt tttgttcgaa
      421 tattcgggga ttctatcttt ggtttcttca acaagttgac cccatttacc agttcttttt
      481 tgctcttcat atactgctgc tcttttgaaa tgtggcatgc cctaaacttc ctcacttctt
      541 cgagcttgta atgaattgct gtttcactgt atctcgctgt cacaatcatt ttcgttctct
      601 tcatttaatg tgttattctc acttgctgtg ttgaaactgt ttcttaattc tgggtttttg
      661 attgtgtatt ggtccttatc attgttaatc cttagatttt ttacatattc ttcaagtttg
      721 ctccaatttt cttcacgttg caccttacgc tgttgagttt cttttacact tgataagtac
      781 aatgcattac agttttcgta caatactggg tttgtttcca gaaaaatttt caatgccttg
      841 aatgccatag catgaattgc cctattccac gaaccagaat tgattgcttg ctcaacaagc
      901 atgtaagggt ctgaaatgct atcttcgcca tttgctgtat ctagctctaa ctgagaagtt
      961 aattcatata atgcaggaaa tataatgggt aggatgactt cggcattttc gatacataag
     1021 tttaagaaat attcattatt ccaataactt aaaacctttt ccgccacttg gaaatgtgga
     1081 gaagaaatac acttagctaa ttgaacaaat aacggaactt ctactttaat aaattccagc
     1141 ggttcgatca cttcaaaaat atcctcgatt tcatttagaa acattatctc ttttgtggaa
     1201 tttatttttg gccaataacg cagtaagccc ataactacct cttcggttaa taaaggatct
     1261 ttttcgagaa attgaacgat acagtaagcc aactgagggt ggtataatga taaacaacgg
     1321 accttgtgta atggtatcaa tatcctcacc aagaaaacct tgtgctcttc ctttaatgga
     1381 agtgcaaatc cattaattat ggaacccaaa atttctaaca attctgccac accgttaaac
     1441 ttctcagtct cataaataaa ttgcaaaaaa atattattca tcgaccgacg aataaagctt
     1501 cttaatgata agaacttccc atatattcta tgcagtgtcg ttttcaaaca gtctctttct
     1561 ctgatatctt cgctatcaaa taattccagt aactttaaaa taaagtcctg atcaatatat
     1621 tgtttagcaa tctgatgatt gaaatcagga ctttccacaa accttaaaaa gaattcgtaa
     1681 acagcttgca tatgaggcca ggctagttcg ttaacaggtt catcttcatc tgggtcataa
     1741 atgtcaccaa ctggatttac tggtggtgga ataggtctaa acagattgat tttgaacatg
     1801 ttcaccacat gagcgtacat ctcattcgtg taagtgaacc tatttgttac aaggaattct
     1861 attagctcat ctaaggtgct tcttttaatc tctttacctt gaatgtcaaa acttggatca
     1921 ttaaagtcaa acatagtgtt acactggtca actttggcaa tgaacaaagg gattctttct
     1981 tcaggagaaa cttcattaaa atttggcaat ttcgttaatg gcgtatatct agatggctcg
     2041 aatctagatg aagaatgacg ttggggagtc ttaattaact ctaaatctgt gtcagggtta
     2101 agttttgtgg gtgttggtag tctctcaaat gagtgtgatg atctcggaat gtcgatactt
     2161 tgtgaggagg cgtggtttgc atcctgagta ttactagtat taatattcat attgttatta
     2221 ttactattac cattttctac atcagaagaa actgctgtac ctgaagcatt tgaaatggga
     2281 gatgatggaa cagcggatac tgcccccagc aaagtaggat gtgcatgatc ttcaccagaa
     2341 gtgcttttgt cgtccttagt aacagtgagt actggagtgg ggttcattat agcggcagat
     2401 gatgaagaag atccttgctt ttgagattgt gatggctgtt gcgattgttt ggactgttct
     2461 tgtccttttc ttgaacttgt tttctttgtg gagcttgaac cggagcgact gacacttgaa
     2521 cttctactgc tactgctgct ggaagaaggg gtcgtcgctg tttttgtttt tgatgtagag
     2581 ctactatgat gcttcccttg cgagggaaca ctaccagatt gcttgccctt ctcagttgta
     2641 gactttgatc cggtactgct ggcagaactg tgagtagtcc cgtgggaaga gctactagcc
     2701 gaagcgggct tctttgatgt ggatgaggta gtggaacttt tttctttttc cttctctttg
     2761 tccttctttt tactgcttga agaagaagaa gacccggtgg tcttctttat taatctttgc
     2821 ttgaaaccac gcatcatatt gaaagtggat gttcgaaaat agcacgtgcc tattgatctt
     2881 aataagtaga acttttatac aaaataaaaa agccagttaa agacggtatt gaggtgaaca
     2941 ggtatatgga gaagggttta tatttttatc taacaaaaaa aggaaaaaaa agcaaaatcg
     3001 aggggggaag gaaatgaaac ccaaaatact tacggcgacg atggtttatc cttgaaatga
     3061 agagttttcc aatgaagaaa cgagacgtat tgacatgcct accaattgaa gtggaaaaca
     3121 aagcgtagtt tcacatactc tttgataatc aattgtctga tacttttttt tttcctttct
     3181 cgcagccggg atagggggtt gtgacccggc aaaaaatgtt ggtctaagtt ttattgttgc
     3241 tatatgctcc atacatacaa taaatgtcta taaaattttt agaagaaaat caggcgtgaa
     3301 agatggtgtc tgcatctttg tggtagagca tcaagtaggc cgtgaatccc ttcccctgcg
     3361 gatcgggttc tcctttggag ttcacttctc caaccccgaa ctgaaacacc aaataggttt
     3421 tgaaaccgaa atattcgtac acagatcttg cgtgctccga aatagcctcg agagcaaggg
     3481 cgcaattggc cttgtctgct ttggttttat atttttcgag tatttttctg actgaacctc
     3541 taatttcggg atggctagga tcttttccga tggcataaag gtagtaatag tccattccca
     3601 gaggcatgac ttcgtgcttc ctcacagtca agtcgtgaaa agggtaccga gctcgaattc
     3661 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     3721 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     3781 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     3841 tacgcatctg tgcggtattt cacaccgcat atatcggatc gtacttgtta cccatcattg
     3901 aattttgaac atccgaacct gggagttttc cctgaaacag atagtatatt tgaacctgta
     3961 taataatata tagtctagcg ctttacggaa gacaatgtat gtatttcggt tcctggagaa
     4021 actattgcat ctattgcata ggtaatcttg cacgtcgcat ccccggttca ttttctgcgt
     4081 ttccatcttg cacttcaata gcatatcttt gttaacgaag catctgtgct tcattttgta
     4141 gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
     4201 acagaacaga aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt
     4261 ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
     4321 tttttacaga acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt
     4381 cttttttgtt ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga
     4441 ttactttttt tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt
     4501 aggtccgtta aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag
     4561 cctgactcca cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag
     4621 ataaaggcat ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga
     4681 aagtgatagc gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt
     4741 gtctctatat actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta
     4801 tgaatagttc ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa
     4861 atgtagaggt cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat
     4921 agggatatag cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg
     4981 gtattcgcaa tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg
     5041 cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagctag
     5101 agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa
     5161 tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg
     5221 cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac
     5281 ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc
     5341 attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg
     5401 ccactcctca attggattag tctcatcctt caatgctatc atttcctttg atattggatc
     5461 gatccgatga taagctgtca aacatgagaa ttaattctac cctatgaaca tattccattt
     5521 tgtaatttcg tgtcgtttct attatgaatt tcatttataa agtttatgta caaatatcat
     5581 aaaaaaagag aatcttttta agcaaggatt ttcttaactt cttcggcgac agcatcaccg
     5641 acttcggtgg tactgttgga accacctaaa tcaccagttc tgatacctgc atccaaaacc
     5701 tttttaactg catcttcaat ggccttacct tcttcaggca agttcaatga caatttcaac
     5761 atcattgcag cagacaagat agtggcgata gggtcaacct tattctttgg caaatctgga
     5821 gcagaaccgt ggcatggttc gtacaaacca aatgcggtgt tcttgtctgg caaagaggcc
     5881 aaggacgcag atggcaacaa acccaaggaa cctgggataa cggaggcttc atcggagatg
     5941 atatcaccaa acatgttgct ggtgattata ataccattta ggtgggttgg gttcttaact
     6001 aggatcatgg cggcagaatc aatcaattga tgttgaacct tcaatgtagg aaattcgttc
     6061 ttgatggttt cctccacagt ttttctccat aatcttgaag aggccaaaac attagcttta
     6121 tccaaggacc aaataggcaa tggtggctca tgttgtaggg ccatgaaagc ggccattctt
     6181 gtgattcttt gcacttctgg aacggtgtat tgttcactat cccaagcgac accatcacca
     6241 tcgtcttcct ttctcttacc aaagtaaata cctcccacta attctctgac aacaacgaag
     6301 tcagtacctt tagcaaattg tggcttgatt ggagataagt ctaaaagaga gtcggatgca
     6361 aagttacatg gtcttaagtt ggcgtacaat tgaagttctt tacggatttt tagtaaacct
     6421 tgttcaggtc taacactacc tgtaccccat ttaggaccac ccacagcacc taacaaaacg
     6481 gcatcaacct tcttggaggc ttccagcgcc tcatctggaa gtgggacacc tgtagcatcg
     6541 atagcagcac caccaattaa atgattttcg aaatcgaact tgacattgga acgaacatca
     6601 gaaatagctt taagaacctt aatggcttcg gctgtgatct tgaccaacgt ggtcacctgg
     6661 caaaacgacg atcttcttag gggcagacat aggggcagac attagaatgt atatccttga
     6721 aatatatata tatattgctg aaatgtaaaa ggtaagaaaa gttagaataa gacgattgct
     6781 aaccacctat tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg
     6841 atgcaagcat ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct
     6901 cacctttcct ttttctccca atttttcagt tgaaaaaggt atatgcgcgg cgacctctga
     6961 aattaacaaa aaatttccag tcatcgaatt tgtctgtgcg atagcgcccc tgtgtgttct
     7021 cgttatgttg aggaaaaaaa taatggttgc taagagattc gaactcttgc atcttacgat
     7081 acctgagtat tcccacagtt aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
     7141 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     7201 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7261 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7321 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7381 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7441 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7501 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7561 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7621 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7681 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     7741 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     7801 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     7861 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     7921 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     7981 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8041 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8101 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8161 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8221 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8281 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8341 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8401 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8461 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     8521 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8581 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8641 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8701 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8761 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8821 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     8881 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     8941 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9001 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9061 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ga
//
