LOCUS       sequence-67192-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mos1 transposase expression vector for use in creating targeted 
            transgene insertions and targeted deletions in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ601 - Peft-3 Mos1 transposase
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..607
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    616..640
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aaagttggca cctttggtct tttattgtca acttccattg gttcttccat tgtttctgtt
       61 aaattaatga atttttcata aaataaagac attatacaat ataaaaatga agaatttatt
      121 gaaaataaac tgccagagag aaaaagtatg caacactccc gccgagagtg tttgaaatgg
      181 tgtacggtac attttcgtgc taggagttag atgtgcaggc agcaacgaga gggggagaga
      241 tttttttggg ccttgtgaaa ttaacgtgag ttttctggtc atctgactaa tcatgttggt
      301 tttttgttgg tttattttgt ttttatcttt gtttttatcc agattaggaa atttaaattt
      361 tatgaattta taatgaggtc aaacattcag tcccagcgtt tttcctgttc tcactgttta
      421 gtcgaatttt tattttaggc tttcaacaaa tgttctaact gtcttatttg tgacctcact
      481 ttttatattt ttttaatttt taaaaatatt agaagtttct aggataattt tttcgacttt
      541 tattctctct accgtccgca ctcttcttac ttttaaatta aattgttttt ttttcagttg
      601 ggaaacactt tgctcacaag tttgtacaaa aaagcaggct taatgtcgag tttcgtgccg
      661 aataaagagc aaacgcggac agtattaatt ttctgttttc atttgaagaa aacagctgcg
      721 gaatcgcacc gaatgcttgt tgaagccttt ggcgaacaag taccaactgt gaaaacgtgt
      781 gaacggtggt ttcaacgctt caaaagtggt gattttgacg tcgacgac
//
