LOCUS       sequence-67194-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     Murray Lab pBEST plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBEST-PLtetO-1-UTR1-TetR-deGFP-T500
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..32)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(36..656)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     564..583
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     RBS             665..687
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    711..729
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    736..754
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 atagcaccac cagactggaa gtacaggttt tcaccagacc cactttcaca tttaagttgt
       61 ttttctaatc cgcatatgat caattcaagg ccgaataaga aggctggctc tgcaccttgg
      121 tgatcaaata attcgatagc ttgtcgtaat aatggcggca tactatcagt agtaggtgtt
      181 tccctttctt ctttagcgac ttgatgctct tgatcttcca atacgcaacc taaagtaaaa
      241 tgccccacag cgctgagtgc atataatgca ttctctagtg aaaaaccttg ttggcataaa
      301 aaggctaatt gattttcgag agtttcatac tgtttttctg taggccgtgt acctaaatgt
      361 acttttgctc catcgcgatg acttagtaaa gcacatctaa aacttttagc gttattacgt
      421 aaaaaatctt gccagctttc cccttctaaa gggcaaaagt gagtatggtg cctatctaac
      481 atctcaatgg ctaaggcgtc gagcaaagcc cgcttatttt ttacatgcca atacaatgta
      541 ggctgctcta cacctagctt ctgggcgagt ttacgggttg ttaaaccttc gattccgacc
      601 tcattaagca gctctaatgc gctgttaatc actttacttt tatctaatct agacatgtgg
      661 tatatctcct tcttaaagtt aaacaaaatt attgctagct gtgctcagta tctctatcac
      721 tgatagggat gtcaatctct atcactgata gggagcatgc ccagcggaac agcacacaca
      781 tgtcgcggtg gttacgtccg tcacgttcac gcatcaggcg gatatcgtta gctgatggtg
      841 cgatagtctt caccatgtca aacatcc
//
