LOCUS       sequence-67197-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  TITLE     Murray Lab pBEST plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBEST-p15a-pBAD-TetO1-UTR1-deGFP-ssrA-T500
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    78..96
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(120..139)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(616..634)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        702..806
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtatatctc cttcttaaag ttaaacaaaa ttattgctag ctgtgctcag tatctctatc
       61 actgataggg atgtcaatct ctatcactga tagggacagg taggatccgc taatcttatg
      121 gataaaaatg ctatggcata gcaaagtgtg acgccgtgca aataatcaat gtggactttt
      181 ctgccgtgat tatagacact tttgttacgc gtttttgtca tggctttggt cccgctttgt
      241 tacagaatgc ttttaataag cggggttacc ggtttggtta gcgagaagag ccagtaaaag
      301 acgcagtgac ggcaatgtct gatgcaatat ggacaattgg tttctgcatg cccagcggaa
      361 cagcacacac atgtcgcggt ggttacgtcc gtcacgttca cgcatcaggc ggatatcgtt
      421 agcccaccca gcaaaattcg gttttctggc tgatggtgcg atagtcttca ccatgtcaaa
      481 catccactct gcggcggtca ggtcttctgc tgtcccccac ttgctggcgc tctgaattgc
      541 agcatccggt ttcaccacag aaaggtcgtt ttctggctgg tcagaggatt cgccagaatt
      601 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
      661 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      721 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      781 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccg
//
