LOCUS       sequence-67198-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  TITLE     Murray Lab pBEST plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBEST-OR2-OR1-Pr-araC, pBAD-TetR,
            pBAD-TetO1-d...
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    62..80
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    87..105
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(129..148)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     669..687
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aagctccatg gtatatctcc ttcttaaagt taaacaaaat tattgctagc tgtgctcagt
       61 atctctatca ctgataggga tgtcaatctc tatcactgat agggacaggt aggatccgct
      121 aatcttatgg ataaaaatgc tatggcatag caaagtgtga cgccgtgcaa ataatcaatg
      181 tggacttttc tgccgtgatt atagacactt ttgttacgcg tttttgtcat ggctttggtc
      241 ccgctttgtt acagaatgct tttaataagc ggggttaccg gtttggttag cgagaagagc
      301 cagtaaaaga cgcagtgacg gcaatgtctg atgcaatatg gacaattggt ttctgcatgc
      361 ccagcggaac agcacacaca tgtcgcggtg gttacgtccg tcacgttcac gcatcaggcg
      421 gatatcgtta gccccgggat cgcagcaaac gacgaaaact acgctttagc tgcttaactc
      481 gagcaaagcc cgccgaaagg cgggcttttc tgtgtcgacc gatgcccttg agagccttca
      541 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
      601 tcttctttat catactagtg ccacctgacg tctaanaaac cattattatc atgacattaa
      661 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattctgg cgaatc
//
