LOCUS       sequence-67200-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  TITLE     Murray Lab pBEST plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBEST-OR2-OR1-Pr-UTR1-araC-T500
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(188..292)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     360..378
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             716..738
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
       61 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
      121 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
      181 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      241 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
      301 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
      361 ataggcgtat cacgaggccc tttcgtcttc aagaattctg gcgaatcctc tgaccagcca
      421 gaaaacgacc tttctgtggt gaaaccggat gctgcaattc agagcgccag caagtggggg
      481 acagcagaag acctgaccgc cgcagagtgg atgtttgaca tggtgaagac tatcgcacca
      541 tcagccagaa aaccgaattt tgctgggtgg gctaacgata tccgcctgat gcgtgaacgt
      601 gacggacgta accaccgcga catgtgtgtg ctgttccgct gggcatgctg agctaacacc
      661 gtgcgtgttg acaattttac ctctggcggt gataatggtt gcagctagca ataattttgt
      721 ttaactttaa gaaggagata taccatgcaa tatggacaat tggtttcttc tctgaatggc
      781 gggagtatga aaagtatggc tgaagcgcaa aa
//
