LOCUS       sequence-67201-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  TITLE     Murray Lab pBEST plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBEST-OR2-OR1-Pr-araC, pBAD-TetR,
            pBAD-TetO1-d...
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        51..155
                     /gene="bla"
                     /label=AmpR promoter
     CDS             340..718
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(374..393)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgctttagct gcttaactcg agcaaagccc ggacttttcg gggaaatgtg cgcggaaccc
       61 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      121 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      181 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      241 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      301 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      361 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
      421 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
      481 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
      541 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
      601 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
      661 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
      721 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
      781 tggaggcgga taaagttg
//
