LOCUS       sequence-67202-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  TITLE     Murray Lab pBEST plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBEST-OR2-OR1-Pr-araC, pBAD-TetR,
            pBAD-TetO1-d...
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             44..66
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(102..121)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             605..627
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cgctgttaat cactttactt ttatctaatc tagacatggt atatctcctt cttaaagtta
       61 aacaaaatta ttgctagcca ggtaggatcc gctaatctta tggataaaaa tgctatggca
      121 tagcaaagtg tgacgccgtg caaataatca atgtggactt ttctgccgtg attatagaca
      181 cttttgttac gcgtttttgt catggctttg gtcccgcttt gttacagaat gcttttaata
      241 agcggggtta ccggtttggt tagcgagaag agccagtaaa agacgcagtg acggcaatgt
      301 ctgatgcaat atggacaatt ggtttctatg ccgtagaatg ctgcaattca gagcgccagc
      361 aagtggggga cagcagaaga cctgaccgcc gcagagtgga tgtttgacat ggtgaagact
      421 atcgcaccat cagccagaaa accgaatttt gctgggtggg ctaacgatat ccgcctgatg
      481 cgtgaacgtg acggacgtaa ccaccgcgac atgtgtgtgc tgttccgctg ggcatgctga
      541 gctaacaccg tgcgtgttga caattttacc tctggcggtg ataatggttg cagctagcaa
      601 taattttgtt taactttaag aaggagatat accatgcaat atggacaatt ggtttcttct
      661 ctgaatggcg ggagtatga
//
