LOCUS       sequence-6721-ex       10188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Malagon F, Tong AH, Shafer BK, Strathern JN
  TITLE     Genetic interactions of DST1 in Saccharomyces cerevisiae suggest a 
            role of TFIIS in the initiation-elongation transition.
  JOURNAL   Genetics 2004 Mar;166(3):1215-27.
  PUBMED    15082542
REFERENCE   2  (bases 1 to 10188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "2004-03"; volume: "166(3)"; pages: "1215-27"
COMMENT     Sequence Label: YEplac181-GAL11
FEATURES             Location/Qualifiers
     source          1..10188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4737..4754)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4737..4753)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4746..4768)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5190..6536
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        complement(7778..8176)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(8196..8214)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8282..8386
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8387..8455,8456..9247)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8605..8624)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9418..10006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9907..9926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10160..10177
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gaggatcccc tcggcctcat caaaacattt cctaggaatt
      301 ggggttggat agatagttac tggtgacacc gatgcatttg atgggttctc ctcgaattta
      361 ggaggataca tggataatcc attagtgata gcccattggt taacttcctg gatcaattca
      421 ttcaattgat ccttggaagg tggatagtgt gccatatttt ataggagtag tgctttcttc
      481 ctataattgc ttgaagtggc actttgctac ctttggagga tcgtatatgg atgtggttga
      541 tttgggagac atttagataa ttcaagaagg ttcaactatt tgtcagcact agctaacccg
      601 gccaaaaaag cgtactgatg gaaatgacac tttctagtac tgatgatagt caagtccatt
      661 ggataaaaag ctagattagg ttatattgta ttgatactca tgtatatata tatatatatt
      721 atataaagaa aaattacatt aaaagtgggg agaaattttt gtgaaataaa gaatttcttt
      781 tctggagtta aaaaagagtt tgaaagaagg agaaaaaaaa gatgttggtg accataacac
      841 caaacgaagt aacttcaaaa gtatcaaaag tatggaaact tcaaatgttc aagtagcact
      901 tgtccaatta ttccaatccc atacattgaa ttgttctgaa ctagtcccag aagttaaaaa
      961 tggagcttca tttgttactt cttgtttttc actagttgtg gctcctgatt ttgtcatgaa
     1021 tgggtcatca ggagaatcta ctttaatttt tttagaatca ctcattatat ttgaatctgg
     1081 tgatagcacc gaggctatcg aatcttgcgg gcttatttct aatacgctgg cttttctttt
     1141 tcttgggttg ccgacatcca tattagaaga tgtggacggc gatgatgaat gaaccaaagt
     1201 ggacaagtct ttaaagttac caccgatacc cttgaatata gcagctacat tggacaacgc
     1261 tatcgaatac gacctattgc tcttattgaa tttactcttc ataaccaatt tgttgtcttt
     1321 tatggaaatg tcaatggaat cttgatcgcg agccctttga gccgcttttg taggcttcct
     1381 tttgccagag ccgttgatgt gatcaaccac agcctttggg atagtgcatg acgtaagcat
     1441 ctcttcatct ttgataccta agcaatcacc cagcgtactc ataaacaaat ccataggaga
     1501 atctttgaaa atttcttggc gatgtttaaa acgagaagat atctccaact tcctgatttc
     1561 caaatctttt aacaagagtt cttcctttct gaatgcattc tctataatgg cactattata
     1621 tgatggttgt gcacccatag gagactgtac agtcttcata ttattggggt ttggtgtgcc
     1681 atttttagtt tgggttttca gtggagaagg atttgtattt ggggttgact tagcggtatt
     1741 tgatgcagac ctaggagtgg gttgttgact agcggcattt gaaattgata gtttcttatt
     1801 tgttgatgga atgctggtaa caggtatcgt attagatttt gtcctgccat tcaccttacc
     1861 gttgatcgtc ttgttcagtg atggggttgc cgcagcggat acgggaactt ttggtgtatt
     1921 ttccatagag ttcaaaaaat ccatgctaga ggtattttta tggttcatat atgggatatt
     1981 gttaccgggt gcattagcca ttgccgaata attagccaca ccaggagtcg aggagggata
     2041 tatgtgctgt tgctgttgtt gttgctgctg ttgttgttgc tgctgttgtt gttgctgctg
     2101 ttgttgttgc tgctgcattt tcaattgctg taaatgctgc atttgctgta gtgactgttg
     2161 catattttgc tgagttgcaa tattattatt gtttccagta gaagtagtgc ctgggttccc
     2221 attgttgttt gccatttgct gttgttgtct taaaatagct tgtcttctta aaatttgaat
     2281 tctcatactt tcccaatatt tttgatactt aataattaac ctgtcaacgg tgtctaattt
     2341 aacaacaaat ataccctcgg cacatttttc taggatctct tttacaaaga ttcttaactg
     2401 aaaaacttcc ttcaagaagt tttcattctt tgtgatgatg tagacttgtg gggcgaaatt
     2461 actcacctga acaaaaagct tttgaccttg ctttaacttc attttaattt tttccttttc
     2521 ttcattggat aaagaatttg ttacgtccgt aagccttagg ttggtcttag aagcatccaa
     2581 ggacaattgc ttaattactt cgacatcctt tgcagtaggt gtcaacccat gtagtgggtt
     2641 aggttgttgc tgagcttgtt gtggttgttg ggagggctgg tgctgagcgt gtgcttgtgc
     2701 ttgtgcttgg gcttgggctt gggcctgcgc ctgggcttgt gcctgtgcct gtgcctgtgc
     2761 ttgtgcttgt gcttgtgctt gtgcttgcgc ttgcgccgct tgtgccgcct gtgcttgagc
     2821 ttgtgcttga gcctgtgcct gagcttgagc ttgagcttga gcttgagctt gttgctgttg
     2881 ttgcatctga tataatttag aacttgcgtt ctgttgctga gagaaagcgg cagcattagg
     2941 tgtagcagaa gtggcaatgg tgtcattatt gttcgtgttc tgttgttgtt gttgttggat
     3001 gatattcata gggacatttt gggctctctg aaggttcgag ccgttgttgt tagcgttatt
     3061 gttgttctta acatctctca aagcttggat ttttctcagg gccttttggt taatgtattt
     3121 cctaataaaa ctttgcttga caggttccgt catcgtacta cccaattgtg ttttttcaaa
     3181 attcttgcag gtttcgatgg cttcttgtaa taagctgcgt tgctcctctg gagaaaagat
     3241 ttggttaatt tggttgagga cgtttggtac ggtattttgt gatgattgct gttgcaaagg
     3301 gttgttgttc gcacttgaat tgggaggttg catttgctgc tgttgaggaa tatttatgtt
     3361 attgttaata ttaccatttt gggggaggcc gttgttgttg ttattagctt gtgcttgagc
     3421 ctgtgcttgt tgttgctgta gccttgcttt gaatagcaac tgctggtgaa tcttgtagac
     3481 ttccttcgca gcttccatat cctgaggtgt caatagcttt tgttgagcca aagcagtgac
     3541 ctgctgccag gtgttgatat tgggtggaat gttaggaatt ctttgcagta attgtttggg
     3601 aataggtgcc actttcatct ggttcactaa ttgttgttgt tgaggagtca attgacgcct
     3661 ctgttgctgc tgctgctgtt gttgttgttg ttgttgattt cttaattgtt gcgcaacctg
     3721 ttgccttgcc tgagcctgct gattcaagaa catctgtggg tttagattca tattgacatt
     3781 catattattg gcgctgttgc cagaattttt caagttgtta atatggtgct gttccacggg
     3841 tttaatgttg ttattagcgg cagcagcagt aacggcgttt ttcctcgtat tgtacgtgtt
     3901 gcgcatgaca gcaacttttt cgttcatgct gtccatgtat tctttctttg aagagctctt
     3961 tgcgaacaaa gctgcctcga agtttttggc atgaattctt atcttatcag cagtgtcgga
     4021 gctccctccg ttcagagtgt taatgtccat gagcacctga agcaacccgt tgacgttctt
     4081 cgcacgctcg gcattggaca gagtgtcttt gtcttggaca ggagcagcag acatagcaga
     4141 tttaaaagaa atagcgtttt aatccttgat ctttgagtac ggcactatac gaaacgatac
     4201 gcttataaaa atggggttta actaacaaag aaaaatatac cagcacaagt atatagcaat
     4261 gtcgtggaag tacttctttg tggaaccagc tgtttccaga gacaaattcc tagtccttgt
     4321 gaatggttga ctattaaaag tttgactgta aaggttaagc accttcatca tcctcatcca
     4381 taatataatt ttctgatcga cgcaaccggg gccggggtgg aaggaaggcc cgattcgaaa
     4441 taaaaaagag gccgccacaa tgtagggtaa tgcctaatta tattaactag agactgactg
     4501 aatcatatta cttagttact actactctta tcatgcaaga agatgtgaaa aagcgtcttc
     4561 ccccaaatca tataacttct gcaccatccg tggactgtac aggttcagcg ggggaatatg
     4621 taattgcttg ttaggattgc tcttgggacc gtagtatcgc gacaaggaag tctgtgtagt
     4681 tccacttgat gtgggctgcc tcgccgcttc ttttgcaggg taccgagctc gaattcactg
     4741 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     4801 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     4861 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
     4921 catctgtgcg gtatttcaca ccgcatatat cggatcgtac ttgttaccca tcattgaatt
     4981 ttgaacatcc gaacctggga gttttccctg aaacagatag tatatttgaa cctgtataat
     5041 aatatatagt ctagcgcttt acggaagaca atgtatgtat ttcggttcct ggagaaacta
     5101 ttgcatctat tgcataggta atcttgcacg tcgcatcccc ggttcatttt ctgcgtttcc
     5161 atcttgcact tcaatagcat atctttgtta acgaagcatc tgtgcttcat tttgtagaac
     5221 aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag
     5281 aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt
     5341 aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt
     5401 tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt
     5461 tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac
     5521 tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt
     5581 ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg
     5641 actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa
     5701 aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt
     5761 gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct
     5821 ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa
     5881 tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt
     5941 agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg
     6001 atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat
     6061 tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc
     6121 ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct agctagagaa
     6181 taggaacttc ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca
     6241 acgcgagctg cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg
     6301 tatatatata tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat
     6361 atgcgtctat ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc
     6421 catgcggggt atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac
     6481 tcctcaattg gattagtctc atccttcaat gctatcattt cctttgatat tggatcgatc
     6541 cgatgataag ctgtcaaaca tgagaattaa ttctacccta tgaacatatt ccattttgta
     6601 atttcgtgtc gtttctatta tgaatttcat ttataaagtt tatgtacaaa tatcataaaa
     6661 aaagagaatc tttttaagca aggattttct taacttcttc ggcgacagca tcaccgactt
     6721 cggtggtact gttggaacca cctaaatcac cagttctgat acctgcatcc aaaacctttt
     6781 taactgcatc ttcaatggcc ttaccttctt caggcaagtt caatgacaat ttcaacatca
     6841 ttgcagcaga caagatagtg gcgatagggt caaccttatt ctttggcaaa tctggagcag
     6901 aaccgtggca tggttcgtac aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg
     6961 acgcagatgg caacaaaccc aaggaacctg ggataacgga ggcttcatcg gagatgatat
     7021 caccaaacat gttgctggtg attataatac catttaggtg ggttgggttc ttaactagga
     7081 tcatggcggc agaatcaatc aattgatgtt gaaccttcaa tgtaggaaat tcgttcttga
     7141 tggtttcctc cacagttttt ctccataatc ttgaagaggc caaaacatta gctttatcca
     7201 aggaccaaat aggcaatggt ggctcatgtt gtagggccat gaaagcggcc attcttgtga
     7261 ttctttgcac ttctggaacg gtgtattgtt cactatccca agcgacacca tcaccatcgt
     7321 cttcctttct cttaccaaag taaatacctc ccactaattc tctgacaaca acgaagtcag
     7381 tacctttagc aaattgtggc ttgattggag ataagtctaa aagagagtcg gatgcaaagt
     7441 tacatggtct taagttggcg tacaattgaa gttctttacg gatttttagt aaaccttgtt
     7501 caggtctaac actacctgta ccccatttag gaccacccac agcacctaac aaaacggcat
     7561 caaccttctt ggaggcttcc agcgcctcat ctggaagtgg gacacctgta gcatcgatag
     7621 cagcaccacc aattaaatga ttttcgaaat cgaacttgac attggaacga acatcagaaa
     7681 tagctttaag aaccttaatg gcttcggctg tgatcttgac caacgtggtc acctggcaaa
     7741 acgacgatct tcttaggggc agacataggg gcagacatta gaatgtatat ccttgaaata
     7801 tatatatata ttgctgaaat gtaaaaggta agaaaagtta gaataagacg attgctaacc
     7861 acctattgga aaaaacaata ggtccttaaa taatattgtc aacttcaagt attgtgatgc
     7921 aagcatttag tcatgaacgc ttctctattc tatatgaaaa gccggttccg gcctctcacc
     7981 tttccttttt ctcccaattt ttcagttgaa aaaggtatat gcgcggcgac ctctgaaatt
     8041 aacaaaaaat ttccagtcat cgaatttgtc tgtgcgatag cgcccctgtg tgttctcgtt
     8101 atgttgagga aaaaaataat ggttgctaag agattcgaac tcttgcatct tacgatacct
     8161 gagtattccc acagttaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     8221 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     8281 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     8341 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     8401 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     8461 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8521 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8581 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     8641 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8701 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8761 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8821 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8881 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8941 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9001 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9061 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9121 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9181 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9241 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     9301 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     9361 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     9421 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     9481 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9541 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     9601 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9661 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9721 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9781 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9841 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9901 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9961 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    10021 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    10081 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
    10141 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaga
//
