LOCUS       sequence-6724-ex       10954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10954)
  AUTHORS   Janicki SM, Tsukamoto T, Salghetti SE, Tansey WP, Sachidanandam R, 
            Prasanth KV, Ried T, Shav-Tal Y, Bertrand E, Singer RH, Spector DL
  TITLE     From silencing to gene expression: real-time analysis in single 
            cells.
  JOURNAL   Cell 2004 Mar 5;116(5):683-98.
  PUBMED    15006351
REFERENCE   2  (bases 1 to 10954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "2004-03-5"; volume: "116(5)"; pages: "683-98"
COMMENT     Sequence Label: CMV-dH3-YFP
FEATURES             Location/Qualifiers
     source          1..10954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3038..3132
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3151..3389
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3336..3357
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     enhancer        3487..3866
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3867..4070
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4021..4041
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4115..4134
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4115..4133
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4586..4595
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4592..4594,4595..4597,4598..5308)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4637..4658)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4898..4917)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5245..5266
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          5428..5493
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5623..5643
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6068..6202
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6105..6124)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6159..6178
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6229..6247)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6707..6778,7173..7446,7521..8175,8237..8552,8773..8904,
                     8975..9589)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(10367..10921,10922..10952)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
     3001 tgcatgagct cggatccaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga
     3061 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     3121 ctgtcctccg agcggagact ctagcgagcg ccggagtata aatagaggcg cttcgtctac
     3181 ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa
     3241 ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt taaagtgaat
     3301 caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc tgccaagaag
     3361 taattattga atacaagaag agaactctga atagggaatt gggaattcca aaacaaactg
     3421 gttattgtgg taggtcattt gtttggcaga aagaaaactc gagtgtacgg gccagatata
     3481 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     3541 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     3601 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     3661 tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc cacttggcag
     3721 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     3781 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     3841 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     3901 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     3961 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     4021 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa
     4081 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
     4141 aagcttggta cctctagagg agatggctcg taccaagcaa actgctcgca aatcgactgg
     4201 tggaaaggcg ccacgcaaac aactggctac taaggccgct cgcaagagtg ctccagccac
     4261 cggaggtgtg aagaagcccc accgctatcg ccctggaacc gtggccttgc gtgaaattcg
     4321 tcgctaccaa aagagcaccg agcttctaat ccgcaagctg cctttccagc gtctggtgcg
     4381 tgaaatcgct caggacttta agacggactt gcgattccag agctcggcgg ttatggctct
     4441 gcaggaagct agcgaagcct acctggttgg tctcttcgaa gataccaact tgtgtgccat
     4501 tcatgccaag cgtatcacca taatgcccaa agacatccag ttagcgcgac gcattcgcgg
     4561 cgagcgtgct cagcggccgc cggtcgccac catggtgagc aagggcgagg agctgttcac
     4621 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     4681 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     4741 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca
     4801 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     4861 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     4921 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     4981 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     5041 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     5101 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     5161 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa
     5221 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     5281 cactctcggc atggacgagc tgtacaagtc acggccagtc gcaacaagat ctgattaagg
     5341 taccactaga ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     5401 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     5461 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     5521 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     5581 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     5641 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     5701 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     5761 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
     5821 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
     5881 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
     5941 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
     6001 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     6061 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6121 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6181 tgtatcttat catgtctgga tcggatccac tagaaggcct tagtatgtat gtaagttaat
     6241 aaaacccttt tttggagaat gtagatttaa aaaaacatat ttttttttta ttttttactg
     6301 cactggacat cattgaactt atctgatcag ttttaaattt acttcgatcc aagggtattt
     6361 gaagtaccag gttctttcga ttacctctca ctcaaaatga cattccactc aaagtcagcg
     6421 ctgtttgcct ccttctctgt ccacagaaat atcgccgtct ctttcgccgc tgcgtccgct
     6481 atctctttcg ccaccgtttg tagcgttacc tagcgtcaat gtccgccttc agttgcactt
     6541 tgtcagcggt ttcgtgacga agctccaagc ggtttacgcc atcaattaaa cacaaagtgc
     6601 tgtgccaaaa ctcctctcgc ttcttatttt tgtttgtttt ttgagtgatt ggggtggtga
     6661 ttggttttgg gtgggtaagc aggggaaagt gtgaaaaatc ccggcaatgg gccaagagga
     6721 tcaggagcta ttaattcgcg gaggcagcaa acacccatct gccgagcatc tgaacaatgt
     6781 gagtagtaca tgtgcataca tcttaagttc acttgatcta taggaactgc gattgcaaca
     6841 tcaaattgtc tgcggcgtga gaactgcgac ccacaaaaat cccaaaccgc aatcgcacaa
     6901 acaaatagtg acacgaaaca gattattctg gtagctgtgc tcgctatata agacaatttt
     6961 taagatcata tcatgatcaa gacatctaaa ggcattcatt ttcgactaca ttctttttta
     7021 caaaaaatat aacaaccaga tattttaagc tgatcctaga tgcacaaaaa ataaataaaa
     7081 gtataaacct acttcgtagg atacttcgtt ttgttcgggg ttagatgagc ataacgcttg
     7141 tagttgatat ttgagatccc ctatcattgc agggtgacag cggagcggct tcgcagagct
     7201 gcattaacca gggcttcggg caggccaaaa actacggcac gctcctgcca cccagtccgc
     7261 cggaggactc cggttcaggg agcggccaac tagccgagaa cctcacctat gcctggcaca
     7321 atatggacat ctttggggcg gtcaatcagc cgggctccgg atggcggcag ctggtcaacc
     7381 ggacacgcgg actattctgc aacgagcgac acataccggc gcccaggaaa catttgctca
     7441 agaacggtga gtttctattc gcagtcggct gatctgtgtg aaatcttaat aaagggtcca
     7501 attaccaatt tgaaactcag tttgcggcgt ggcctatccg ggcgaacttt tggccgtgat
     7561 gggcagttcc ggtgccggaa agacgaccct gctgaatgcc cttgcctttc gatcgccgca
     7621 gggcatccaa gtatcgccat ccgggatgcg actgctcaat ggccaacctg tggacgccaa
     7681 ggagatgcag gccaggtgcg cctatgtcca gcaggatgac ctctttatcg gctccctaac
     7741 ggccagggaa cacctgattt tccaggccat ggtgcggatg ccacgacatc tgacctatcg
     7801 gcagcgagtg gcccgcgtgg atcaggtgat ccaggagctt tcgctcagca aatgtcagca
     7861 cacgatcatc ggtgtgcccg gcagggtgaa aggtctgtcc ggcggagaaa ggaagcgtct
     7921 ggcattcgcc tccgaggcac taaccgatcc gccgcttctg atctgcgatg agcccacctc
     7981 cggactggac tcatttaccg cccacagcgt cgtccaggtg ctgaagaagc tgtcgcagaa
     8041 gggcaagacc gtcatcctga ccattcatca gccgtcttcc gagctgtttg agctctttga
     8101 caagatcctt ctgatggccg agggcagggt agctttcttg ggcactccca gcgaagccgt
     8161 cgacttcttt tcctagtgag ttcgatgtgt ttattaaggg tatctagcat tacattacat
     8221 ctcaactcct atccagcgtg ggtgcccagt gtcctaccaa ctacaatccg gcggactttt
     8281 acgtacaggt gttggccgtt gtgcccggac gggagatcga gtcccgtgat cggatcgcca
     8341 agatatgcga caattttgct attagcaaag tagcccggga tatggagcag ttgttggcca
     8401 ccaaaaattt ggagaagcca ctggagcagc cggagaatgg gtacacctac aaggccacct
     8461 ggttcatgca gttccgggcg gtcctgtggc gatcctggct gtcggtgctc aaggaaccac
     8521 tcctcgtaaa agtgcgactt attcagacaa cggtgagtgg ttccagtgga aacaaatgat
     8581 ataacgctta caattcttgg aaacaaattc gctagatttt agttagaatt gcctgattcc
     8641 acacccttct tagttttttt caatgagatg tatagtttat agttttgcag aaaataaata
     8701 aatttcattt aactcgcgaa catgttgaag atatgaatat taatgagatg cgagtaacat
     8761 tttaatttgc agatggttgc catcttgatt ggcctcatct ttttgggcca acaactcacg
     8821 caagtgggcg tgatgaatat caacggagcc atcttcctct tcctgaccaa catgaccttt
     8881 caaaacgtct ttgccacgat aaatgtaagt cttgtttaga atacatttgc atattaataa
     8941 tttactaact ttctaatgaa tcgattcgat ttaggtgttc acctcagagc tgccagtttt
     9001 tatgagggag gcccgaagtc gactttatcg ctgtgacaca tactttctgg gcaaaacgat
     9061 tgccgaatta ccgctttttc tcacagtgcc actggtcttc acggcgattg cctatccgat
     9121 gatcggactg cgggccggag tgctgcactt cttcaactgc ctggcgctgg tcactctggt
     9181 ggccaatgtg tcaacgtcct tcggatatct aatatcctgc gccagctcct cgacctcgat
     9241 ggcgctgtct gtgggtccgc cggttatcat accattcctg ctctttggcg gcttcttctt
     9301 gaactcgggc tcggtgccag tatacctcaa atggttgtcg tacctctcat ggttccgtta
     9361 cgccaacgag ggtctgctga ttaaccaatg ggcggacgtg gagccgggcg aaattagctg
     9421 cacatcgtcg aacaccacgt gccccagttc gggcaaggtc atcctggaga cgcttaactt
     9481 ctccgccgcc gatctgccgc tggactacgt gggtctggcc attctcatcg tgagcttccg
     9541 ggtgctcgca tatctggctc taagacttcg ggcccgacgc aaggagtagc cgacatatat
     9601 ccgaaataac tgcttgtttt tttttttacc attattacca tcgtgtttac tgtttattgc
     9661 cccctcaaaa agctaatgta attatatttg tgccaataaa aacaagatat gacctataga
     9721 atacaagtat ttccccttcg aacatcccca caagtagact ttggatttgt cttctaacca
     9781 aaagacttac acacctgcat accttacatc aaaaactcgt ttatcgctac ataaaacacc
     9841 gggatatatt ttttatatac atacttttca aatcgcgcgc cctcttcata attcacctcc
     9901 accacaccac gtttcgtagt tgctctttcg ctgtctccca cccgctctcc gcaacacatt
     9961 caccttttgt tcgacgacct tggagcgact gtcgttagtt ccgcgcgatt cggttcgctc
    10021 aaatggttcc gagtggttca tttcgtctca atagaaatta gtaataaata tttgtatgta
    10081 caatttattt gctccaatat atttgtatat atttccctca cagctatatt tattctaatt
    10141 taatattatg actttttaag gtaatttttt gtgacctgtt cggagtgatt agcgttacaa
    10201 tttgaactga aagtgacatc cagtgtttgt tccttgtgta gatgcatctc aaaaaaatgg
    10261 tgggcataat agtgttgttt atatatatca aaaataacaa ctataataat aagaatacat
    10321 ttaatttaga aaatgcttgg atttcactgg aactagaatt aattcggctg ctgctctaaa
    10381 cgacgcattt cgtactccaa agtacgaatt ttttccctca agctcttatt ttcattaaac
    10441 aatgaacagg acctaacgca cagtcacgtt attgtttaca taaatgattt tttttactat
    10501 tcaaacttac tctgtttgtg tactcccact ggtatagcct tcttttatct tttctggttc
    10561 aggctctatc actttactag gtacggcatc tgcgttgagt cgcctccttt taaatgtctg
    10621 accttttgca ggtgcagcct tccactgcga atcattaaag tgggtatcac aaatttggga
    10681 gttttcacca aggctgcacc caaggctctg ctcccacaat tttctcttaa tagcacactt
    10741 cggcacgtga attaatttta ctccagtcac agctttgcag caaaatttgc aatatttcat
    10801 ttttttttat tccacgtaag ggttaatgtt ttcaaaaaaa aattcgtccg cacacaacct
    10861 ttcctctcaa caagcaaacg tgcactgaat ttaagtgtat acttcggtaa gcttcggcta
    10921 tcgacgggac caccttatgt tatttcatca tgat
//
