LOCUS       sequence-6726-ex       10674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10674)
  AUTHORS   Ahmad K, Henikoff S
  TITLE     The histone variant H3.3 marks active chromatin by 
            replication-independent nucleosome assembly.
  JOURNAL   Mol Cell 2002 Jun;9(6):1191-200.
  PUBMED    12086617
REFERENCE   2  (bases 1 to 10674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell"; 
            date: "2002-06"; volume: "9(6)"; pages: "1191-200"
COMMENT     Sequence Label: HS-H3.3A-GFP
FEATURES             Location/Qualifiers
     source          1..10674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(236..824)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(316..335)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(995..1786,1787..1855))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1618..1637
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1856..1960)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2028..2046
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2084..2106)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2206..2225
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2762..2792,2793..2994)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3039..3133
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3152..3390
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     promoter        3615..3853
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             4344..5060
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4374..4402)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4997..5017
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     intron          5150..5215
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5345..5365
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5790..5924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5827..5846)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5881..5900
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5951..5969)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6429..6500,6895..7168,7243..7897,7959..8274,8495..8626,
                     8697..9311)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(10089..10643,10644..10674)
                     /label=P element 5' end
ORIGIN
        1 cggccagacc cacgtagtcc agcggcagat cggcggcgga gaagttaagc gtctccagga
       61 tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt gcagctaatt tcgcccggct
      121 ccacgtccgc ccattggtta atcagcagac cctcgttggc gtaacggaac catgagaggt
      181 acgacaacca tttgaggtat actggcaccg agcccgagtt caagaagaag gcgtttttcc
      241 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      301 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      361 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      421 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      481 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      541 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      601 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      661 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      721 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      781 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      841 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
      901 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
      961 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     1021 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     1081 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     1141 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     1201 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     1261 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     1321 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     1381 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     1441 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     1501 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     1561 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     1621 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1681 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1741 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1801 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     1861 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1921 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     1981 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     2041 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     2101 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     2161 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     2221 ttgtactgag agtgcaccat atgcggtgtg aaataccgca ccgaatcgcg cggaactaac
     2281 gacagtcgct ccaaggtcgt cgaacaaaag gtgaatgtgt tgcggagagc gggtgggaga
     2341 cagcgaaaga gcaactacga aacgtggtgt ggtggaggtg aattatgaag agggcgcgcg
     2401 atttgaaaag tatgtatata aaaaatatat cccggtgttt tatgtagcga taaacgagtt
     2461 tttgatgtaa ggtatgcagg tgtgtaagtc ttttggttag aagacaaatc caaagtctac
     2521 ttgtggggat gttcgaaggg gaaatacttg tattctatag gtcatatctt gtttttattg
     2581 gcacaaatat aattacatta gctttttgag ggggcaataa acagtaaaca cgatggtaat
     2641 aatggtaaaa aaaaaaacaa gcagttattt cggatatatg tcggctactc cttgcgtcgg
     2701 gcccgaagtc ttagagccag atatgcgagc acccggaagc tcacgatgag aatggccaga
     2761 ccatgatgaa ataacataag gtggtcccgt cggcaagaga catccactta acgtatgctt
     2821 gcaataagtg cgagtgaaag gaatagtatt ctgagtgtcg tattgagtct gagtgagaca
     2881 gcgatatgat tgttgattaa cccttagcat gtccgtgggg tttgaattaa ctcataatat
     2941 taattagacg aaattatttt taaagtttta tttttaataa tttgcgagta cgcaaagctt
     3001 ctgcatgagc tcggatccaa gcttgcatgc ctgcaggtcg gagtactgtc ctccgagcgg
     3061 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
     3121 actgtcctcc gagcggagac tctagcgagc gccggagtat aaatagaggc gcttcgtcta
     3181 cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca
     3241 aataaacaag cgcagctgaa caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa
     3301 tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa
     3361 gtaattattg aatacaagaa gagaactctg aatagggaat tgggaattcc aaaacaaact
     3421 ggttattgtg gtaggtcatt tgtttggcag aaagaaaact cgagaaattt ctctggccgt
     3481 tattcgttat tctctctttt ctttttgggt ctctccctct ctgcactaat gctctctcac
     3541 tctgtcacac agtaaacggc atactgctct cgttggttcg agagagcgcg cctcgaatgt
     3601 tcgcgaaaag agcgccggag tataaataga ggcgcttcgt ctacggagcg acaattcaat
     3661 tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct
     3721 gaacaagcta aacaatctgc agtaaagtgc aagttaaagt gaatcaatta aaagtaacca
     3781 gcaaccaagt aaatcaactg caactactga aatctgccaa gaagtaatta ttgaatacaa
     3841 gaagagaact ctgggggatc tgatcaagag acaggatgag gtctagagct caaaaccact
     3901 taattaccag gtaaacgatg gcacgtacca agcaaacagc ccgtaaatcg accggaggca
     3961 aggcgccccg caagcagctg gccaccaagg cggcccgtaa atcggcgcca tccaccggcg
     4021 gagtgaagaa gccacatcgc taccgtcctg gaacggtggc cctgcgtgag attcgtcgct
     4081 accagaagtc cacggagctg ctcatccgca agctgccgtt ccagcgtctg gtgcgcgaga
     4141 tagcccagga cttcaagacc gatctgcgct tccagtcggc ggccattgga gccctacagg
     4201 aggccagcga ggcgtacctg gtcggtctgt tcgaggacac caatctgtgc gccattcacg
     4261 ccaagcgcgt caccattatg cccaaggaca tccagctggc cagacgcatc cgtggcgagc
     4321 gggcctcacg gccggtcgca acaatgagta aaggagaaga acttttcact ggagttgtcc
     4381 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
     4441 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
     4501 tacctgttcc atggccaaca cttgtcacta ctttcactta tggtgttcaa tgcttttcaa
     4561 gatacccaga tcatatgaag cggcacgact tcttcaagag cgccatgcct gagggatacg
     4621 tgcaggagag gaccatcttc ttcaaggacg acgggaacta caagacacgt gctgaagtca
     4681 agtttgaggg agacaccctc gtcaacagga tcgagcttaa gggaatcgat ttcaaggagg
     4741 acggaaacat cctcggccac aagttggaat acaactacaa ctcccacaac gtatacatca
     4801 tggccgacaa gcaaaagaac ggcatcaaag ccaacttcaa gacccgccac aacatcgaag
     4861 acggcggcgt gcaactcgct gatcattatc aacaaaatac tccaattggc gatggccctg
     4921 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
     4981 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     5041 tggatgaact atacaaataa agatccacta gaggatcttt gtgaaggaac cttacttctg
     5101 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
     5161 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
     5221 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
     5281 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
     5341 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
     5401 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
     5461 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat
     5521 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag
     5581 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa
     5641 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata
     5701 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     5761 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     5821 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5881 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcggatcc actagaaggc
     5941 cttagtatgt atgtaagtta ataaaaccct tttttggaga atgtagattt aaaaaaacat
     6001 attttttttt tattttttac tgcactggac atcattgaac ttatctgatc agttttaaat
     6061 ttacttcgat ccaagggtat ttgaagtacc aggttctttc gattacctct cactcaaaat
     6121 gacattccac tcaaagtcag cgctgtttgc ctccttctct gtccacagaa atatcgccgt
     6181 ctctttcgcc gctgcgtccg ctatctcttt cgccaccgtt tgtagcgtta cctagcgtca
     6241 atgtccgcct tcagttgcac tttgtcagcg gtttcgtgac gaagctccaa gcggtttacg
     6301 ccatcaatta aacacaaagt gctgtgccaa aactcctctc gcttcttatt tttgtttgtt
     6361 ttttgagtga ttggggtggt gattggtttt gggtgggtaa gcaggggaaa gtgtgaaaaa
     6421 tcccggcaat gggccaagag gatcaggagc tattaattcg cggaggcagc aaacacccat
     6481 ctgccgagca tctgaacaat gtgagtagta catgtgcata catcttaagt tcacttgatc
     6541 tataggaact gcgattgcaa catcaaattg tctgcggcgt gagaactgcg acccacaaaa
     6601 atcccaaacc gcaatcgcac aaacaaatag tgacacgaaa cagattattc tggtagctgt
     6661 gctcgctata taagacaatt tttaagatca tatcatgatc aagacatcta aaggcattca
     6721 ttttcgacta cattcttttt tacaaaaaat ataacaacca gatattttaa gctgatccta
     6781 gatgcacaaa aaataaataa aagtataaac ctacttcgta ggatacttcg ttttgttcgg
     6841 ggttagatga gcataacgct tgtagttgat atttgagatc ccctatcatt gcagggtgac
     6901 agcggagcgg cttcgcagag ctgcattaac cagggcttcg ggcaggccaa aaactacggc
     6961 acgctcctgc cacccagtcc gccggaggac tccggttcag ggagcggcca actagccgag
     7021 aacctcacct atgcctggca caatatggac atctttgggg cggtcaatca gccgggctcc
     7081 ggatggcggc agctggtcaa ccggacacgc ggactattct gcaacgagcg acacataccg
     7141 gcgcccagga aacatttgct caagaacggt gagtttctat tcgcagtcgg ctgatctgtg
     7201 tgaaatctta ataaagggtc caattaccaa tttgaaactc agtttgcggc gtggcctatc
     7261 cgggcgaact tttggccgtg atgggcagtt ccggtgccgg aaagacgacc ctgctgaatg
     7321 cccttgcctt tcgatcgccg cagggcatcc aagtatcgcc atccgggatg cgactgctca
     7381 atggccaacc tgtggacgcc aaggagatgc aggccaggtg cgcctatgtc cagcaggatg
     7441 acctctttat cggctcccta acggccaggg aacacctgat tttccaggcc atggtgcgga
     7501 tgccacgaca tctgacctat cggcagcgag tggcccgcgt ggatcaggtg atccaggagc
     7561 tttcgctcag caaatgtcag cacacgatca tcggtgtgcc cggcagggtg aaaggtctgt
     7621 ccggcggaga aaggaagcgt ctggcattcg cctccgaggc actaaccgat ccgccgcttc
     7681 tgatctgcga tgagcccacc tccggactgg actcatttac cgcccacagc gtcgtccagg
     7741 tgctgaagaa gctgtcgcag aagggcaaga ccgtcatcct gaccattcat cagccgtctt
     7801 ccgagctgtt tgagctcttt gacaagatcc ttctgatggc cgagggcagg gtagctttct
     7861 tgggcactcc cagcgaagcc gtcgacttct tttcctagtg agttcgatgt gtttattaag
     7921 ggtatctagc attacattac atctcaactc ctatccagcg tgggtgccca gtgtcctacc
     7981 aactacaatc cggcggactt ttacgtacag gtgttggccg ttgtgcccgg acgggagatc
     8041 gagtcccgtg atcggatcgc caagatatgc gacaattttg ctattagcaa agtagcccgg
     8101 gatatggagc agttgttggc caccaaaaat ttggagaagc cactggagca gccggagaat
     8161 gggtacacct acaaggccac ctggttcatg cagttccggg cggtcctgtg gcgatcctgg
     8221 ctgtcggtgc tcaaggaacc actcctcgta aaagtgcgac ttattcagac aacggtgagt
     8281 ggttccagtg gaaacaaatg atataacgct tacaattctt ggaaacaaat tcgctagatt
     8341 ttagttagaa ttgcctgatt ccacaccctt cttagttttt ttcaatgaga tgtatagttt
     8401 atagttttgc agaaaataaa taaatttcat ttaactcgcg aacatgttga agatatgaat
     8461 attaatgaga tgcgagtaac attttaattt gcagatggtt gccatcttga ttggcctcat
     8521 ctttttgggc caacaactca cgcaagtggg cgtgatgaat atcaacggag ccatcttcct
     8581 cttcctgacc aacatgacct ttcaaaacgt ctttgccacg ataaatgtaa gtcttgttta
     8641 gaatacattt gcatattaat aatttactaa ctttctaatg aatcgattcg atttaggtgt
     8701 tcacctcaga gctgccagtt tttatgaggg aggcccgaag tcgactttat cgctgtgaca
     8761 catactttct gggcaaaacg attgccgaat taccgctttt tctcacagtg ccactggtct
     8821 tcacggcgat tgcctatccg atgatcggac tgcgggccgg agtgctgcac ttcttcaact
     8881 gcctggcgct ggtcactctg gtggccaatg tgtcaacgtc cttcggatat ctaatatcct
     8941 gcgccagctc ctcgacctcg atggcgctgt ctgtgggtcc gccggttatc ataccattcc
     9001 tgctctttgg cggcttcttc ttgaactcgg gctcggtgcc agtatacctc aaatggttgt
     9061 cgtacctctc atggttccgt tacgccaacg agggtctgct gattaaccaa tgggcggacg
     9121 tggagccggg cgaaattagc tgcacatcgt cgaacaccac gtgccccagt tcgggcaagg
     9181 tcatcctgga gacgcttaac ttctccgccg ccgatctgcc gctggactac gtgggtctgg
     9241 ccattctcat cgtgagcttc cgggtgctcg catatctggc tctaagactt cgggcccgac
     9301 gcaaggagta gccgacatat atccgaaata actgcttgtt ttttttttta ccattattac
     9361 catcgtgttt actgtttatt gccccctcaa aaagctaatg taattatatt tgtgccaata
     9421 aaaacaagat atgacctata gaatacaagt atttcccctt cgaacatccc cacaagtaga
     9481 ctttggattt gtcttctaac caaaagactt acacacctgc ataccttaca tcaaaaactc
     9541 gtttatcgct acataaaaca ccgggatata ttttttatat acatactttt caaatcgcgc
     9601 gccctcttca taattcacct ccaccacacc acgtttcgta gttgctcttt cgctgtctcc
     9661 cacccgctct ccgcaacaca ttcacctttt gttcgacgac cttggagcga ctgtcgttag
     9721 ttccgcgcga ttcggttcgc tcaaatggtt ccgagtggtt catttcgtct caatagaaat
     9781 tagtaataaa tatttgtatg tacaatttat ttgctccaat atatttgtat atatttccct
     9841 cacagctata tttattctaa tttaatatta tgacttttta aggtaatttt ttgtgacctg
     9901 ttcggagtga ttagcgttac aatttgaact gaaagtgaca tccagtgttt gttccttgtg
     9961 tagatgcatc tcaaaaaaat ggtgggcata atagtgttgt ttatatatat caaaaataac
    10021 aactataata ataagaatac atttaattta gaaaatgctt ggatttcact ggaactagaa
    10081 ttaattcggc tgctgctcta aacgacgcat ttcgtactcc aaagtacgaa ttttttccct
    10141 caagctctta ttttcattaa acaatgaaca ggacctaacg cacagtcacg ttattgttta
    10201 cataaatgat tttttttact attcaaactt actctgtttg tgtactccca ctggtatagc
    10261 cttcttttat cttttctggt tcaggctcta tcactttact aggtacggca tctgcgttga
    10321 gtcgcctcct tttaaatgtc tgaccttttg caggtgcagc cttccactgc gaatcattaa
    10381 agtgggtatc acaaatttgg gagttttcac caaggctgca cccaaggctc tgctcccaca
    10441 attttctctt aatagcacac ttcggcacgt gaattaattt tactccagtc acagctttgc
    10501 agcaaaattt gcaatatttc attttttttt attccacgta agggttaatg ttttcaaaaa
    10561 aaaattcgtc cgcacacaac ctttcctctc aacaagcaaa cgtgcactga atttaagtgt
    10621 atacttcggt aagcttcggc tatcgacggg accaccttat gttatttcat catg
//
