LOCUS       sequence-6727-ex       10542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10542)
  AUTHORS   Ahmad K, Henikoff S
  TITLE     The histone variant H3.3 marks active chromatin by 
            replication-independent nucleosome assembly.
  JOURNAL   Mol Cell 2002 Jun;9(6):1191-200.
  PUBMED    12086617
REFERENCE   2  (bases 1 to 10542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell"; 
            date: "2002-06"; volume: "9(6)"; pages: "1191-200"
COMMENT     Sequence Label: HS-H3.3A d436-GFP
FEATURES             Location/Qualifiers
     source          1..10542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(236..824)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(316..335)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(995..1786,1787..1855))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1618..1637
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1856..1960)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2028..2046
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2084..2106)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2206..2225
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2762..2792,2793..2994)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3039..3133
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3152..3390
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     promoter        3615..3853
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             4212..4928
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4242..4270)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4865..4885
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     intron          5018..5083
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5213..5233
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5658..5792
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5695..5714)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5749..5768
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5819..5837)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6297..6368,6763..7036,7111..7765,7827..8142,8363..8494,
                     8565..9179)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9957..10511,10512..10542)
                     /label=P element 5' end
ORIGIN
        1 cggccagacc cacgtagtcc agcggcagat cggcggcgga gaagttaagc gtctccagga
       61 tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt gcagctaatt tcgcccggct
      121 ccacgtccgc ccattggtta atcagcagac cctcgttggc gtaacggaac catgagaggt
      181 acgacaacca tttgaggtat actggcaccg agcccgagtt caagaagaag gcgtttttcc
      241 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      301 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      361 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      421 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      481 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      541 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      601 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      661 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      721 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      781 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      841 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
      901 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
      961 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     1021 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     1081 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     1141 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     1201 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     1261 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     1321 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     1381 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     1441 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     1501 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     1561 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     1621 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1681 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1741 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1801 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     1861 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1921 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     1981 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     2041 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     2101 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     2161 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     2221 ttgtactgag agtgcaccat atgcggtgtg aaataccgca ccgaatcgcg cggaactaac
     2281 gacagtcgct ccaaggtcgt cgaacaaaag gtgaatgtgt tgcggagagc gggtgggaga
     2341 cagcgaaaga gcaactacga aacgtggtgt ggtggaggtg aattatgaag agggcgcgcg
     2401 atttgaaaag tatgtatata aaaaatatat cccggtgttt tatgtagcga taaacgagtt
     2461 tttgatgtaa ggtatgcagg tgtgtaagtc ttttggttag aagacaaatc caaagtctac
     2521 ttgtggggat gttcgaaggg gaaatacttg tattctatag gtcatatctt gtttttattg
     2581 gcacaaatat aattacatta gctttttgag ggggcaataa acagtaaaca cgatggtaat
     2641 aatggtaaaa aaaaaaacaa gcagttattt cggatatatg tcggctactc cttgcgtcgg
     2701 gcccgaagtc ttagagccag atatgcgagc acccggaagc tcacgatgag aatggccaga
     2761 ccatgatgaa ataacataag gtggtcccgt cggcaagaga catccactta acgtatgctt
     2821 gcaataagtg cgagtgaaag gaatagtatt ctgagtgtcg tattgagtct gagtgagaca
     2881 gcgatatgat tgttgattaa cccttagcat gtccgtgggg tttgaattaa ctcataatat
     2941 taattagacg aaattatttt taaagtttta tttttaataa tttgcgagta cgcaaagctt
     3001 ctgcatgagc tcggatccaa gcttgcatgc ctgcaggtcg gagtactgtc ctccgagcgg
     3061 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
     3121 actgtcctcc gagcggagac tctagcgagc gccggagtat aaatagaggc gcttcgtcta
     3181 cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca
     3241 aataaacaag cgcagctgaa caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa
     3301 tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa
     3361 gtaattattg aatacaagaa gagaactctg aatagggaat tgggaattcc aaaacaaact
     3421 ggttattgtg gtaggtcatt tgtttggcag aaagaaaact cgagaaattt ctctggccgt
     3481 tattcgttat tctctctttt ctttttgggt ctctccctct ctgcactaat gctctctcac
     3541 tctgtcacac agtaaacggc atactgctct cgttggttcg agagagcgcg cctcgaatgt
     3601 tcgcgaaaag agcgccggag tataaataga ggcgcttcgt ctacggagcg acaattcaat
     3661 tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct
     3721 gaacaagcta aacaatctgc agtaaagtgc aagttaaagt gaatcaatta aaagtaacca
     3781 gcaaccaagt aaatcaactg caactactga aatctgccaa gaagtaatta ttgaatacaa
     3841 gaagagaact ctgggggatc tgatcaagag acaggatgag gtctagaatg gcacgtaaga
     3901 agccacatcg ctaccgtcct ggaacggtgg ccctgcgtga gattcgtcgc taccagaagt
     3961 ccacggagct gctcatccgc aagctgccgt tccagcgtct ggtgcgcgag atagcccagg
     4021 acttcaagac cgatctgcgc ttccagtcgg cggccattgg agccctacag gaggccagcg
     4081 aggcgtacct ggtcggtctg ttcgaggaca ccaatctgtg cgccattcac gccaagcgcg
     4141 tcaccattat gcccaaggac atccagctgg ccagacgcat ccgtggcgag cgggcccggc
     4201 cggtcgcaac aatgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
     4261 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
     4321 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
     4381 ggccaacact tgtcactact ttcacttatg gtgttcaatg cttttcaaga tacccagatc
     4441 atatgaagcg gcacgacttc ttcaagagcg ccatgcctga gggatacgtg caggagagga
     4501 ccatcttctt caaggacgac gggaactaca agacacgtgc tgaagtcaag tttgagggag
     4561 acaccctcgt caacaggatc gagcttaagg gaatcgattt caaggaggac ggaaacatcc
     4621 tcggccacaa gttggaatac aactacaact cccacaacgt atacatcatg gccgacaagc
     4681 aaaagaacgg catcaaagcc aacttcaaga cccgccacaa catcgaagac ggcggcgtgc
     4741 aactcgctga tcattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
     4801 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
     4861 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
     4921 acaaataaag atccactaga ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     4981 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     5041 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     5101 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     5161 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     5221 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     5281 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     5341 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
     5401 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
     5461 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
     5521 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
     5581 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     5641 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5701 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5761 aactcatcaa tgtatcttat catgtctgga tcggatccac tagaaggcct tagtatgtat
     5821 gtaagttaat aaaacccttt tttggagaat gtagatttaa aaaaacatat ttttttttta
     5881 ttttttactg cactggacat cattgaactt atctgatcag ttttaaattt acttcgatcc
     5941 aagggtattt gaagtaccag gttctttcga ttacctctca ctcaaaatga cattccactc
     6001 aaagtcagcg ctgtttgcct ccttctctgt ccacagaaat atcgccgtct ctttcgccgc
     6061 tgcgtccgct atctctttcg ccaccgtttg tagcgttacc tagcgtcaat gtccgccttc
     6121 agttgcactt tgtcagcggt ttcgtgacga agctccaagc ggtttacgcc atcaattaaa
     6181 cacaaagtgc tgtgccaaaa ctcctctcgc ttcttatttt tgtttgtttt ttgagtgatt
     6241 ggggtggtga ttggttttgg gtgggtaagc aggggaaagt gtgaaaaatc ccggcaatgg
     6301 gccaagagga tcaggagcta ttaattcgcg gaggcagcaa acacccatct gccgagcatc
     6361 tgaacaatgt gagtagtaca tgtgcataca tcttaagttc acttgatcta taggaactgc
     6421 gattgcaaca tcaaattgtc tgcggcgtga gaactgcgac ccacaaaaat cccaaaccgc
     6481 aatcgcacaa acaaatagtg acacgaaaca gattattctg gtagctgtgc tcgctatata
     6541 agacaatttt taagatcata tcatgatcaa gacatctaaa ggcattcatt ttcgactaca
     6601 ttctttttta caaaaaatat aacaaccaga tattttaagc tgatcctaga tgcacaaaaa
     6661 ataaataaaa gtataaacct acttcgtagg atacttcgtt ttgttcgggg ttagatgagc
     6721 ataacgcttg tagttgatat ttgagatccc ctatcattgc agggtgacag cggagcggct
     6781 tcgcagagct gcattaacca gggcttcggg caggccaaaa actacggcac gctcctgcca
     6841 cccagtccgc cggaggactc cggttcaggg agcggccaac tagccgagaa cctcacctat
     6901 gcctggcaca atatggacat ctttggggcg gtcaatcagc cgggctccgg atggcggcag
     6961 ctggtcaacc ggacacgcgg actattctgc aacgagcgac acataccggc gcccaggaaa
     7021 catttgctca agaacggtga gtttctattc gcagtcggct gatctgtgtg aaatcttaat
     7081 aaagggtcca attaccaatt tgaaactcag tttgcggcgt ggcctatccg ggcgaacttt
     7141 tggccgtgat gggcagttcc ggtgccggaa agacgaccct gctgaatgcc cttgcctttc
     7201 gatcgccgca gggcatccaa gtatcgccat ccgggatgcg actgctcaat ggccaacctg
     7261 tggacgccaa ggagatgcag gccaggtgcg cctatgtcca gcaggatgac ctctttatcg
     7321 gctccctaac ggccagggaa cacctgattt tccaggccat ggtgcggatg ccacgacatc
     7381 tgacctatcg gcagcgagtg gcccgcgtgg atcaggtgat ccaggagctt tcgctcagca
     7441 aatgtcagca cacgatcatc ggtgtgcccg gcagggtgaa aggtctgtcc ggcggagaaa
     7501 ggaagcgtct ggcattcgcc tccgaggcac taaccgatcc gccgcttctg atctgcgatg
     7561 agcccacctc cggactggac tcatttaccg cccacagcgt cgtccaggtg ctgaagaagc
     7621 tgtcgcagaa gggcaagacc gtcatcctga ccattcatca gccgtcttcc gagctgtttg
     7681 agctctttga caagatcctt ctgatggccg agggcagggt agctttcttg ggcactccca
     7741 gcgaagccgt cgacttcttt tcctagtgag ttcgatgtgt ttattaaggg tatctagcat
     7801 tacattacat ctcaactcct atccagcgtg ggtgcccagt gtcctaccaa ctacaatccg
     7861 gcggactttt acgtacaggt gttggccgtt gtgcccggac gggagatcga gtcccgtgat
     7921 cggatcgcca agatatgcga caattttgct attagcaaag tagcccggga tatggagcag
     7981 ttgttggcca ccaaaaattt ggagaagcca ctggagcagc cggagaatgg gtacacctac
     8041 aaggccacct ggttcatgca gttccgggcg gtcctgtggc gatcctggct gtcggtgctc
     8101 aaggaaccac tcctcgtaaa agtgcgactt attcagacaa cggtgagtgg ttccagtgga
     8161 aacaaatgat ataacgctta caattcttgg aaacaaattc gctagatttt agttagaatt
     8221 gcctgattcc acacccttct tagttttttt caatgagatg tatagtttat agttttgcag
     8281 aaaataaata aatttcattt aactcgcgaa catgttgaag atatgaatat taatgagatg
     8341 cgagtaacat tttaatttgc agatggttgc catcttgatt ggcctcatct ttttgggcca
     8401 acaactcacg caagtgggcg tgatgaatat caacggagcc atcttcctct tcctgaccaa
     8461 catgaccttt caaaacgtct ttgccacgat aaatgtaagt cttgtttaga atacatttgc
     8521 atattaataa tttactaact ttctaatgaa tcgattcgat ttaggtgttc acctcagagc
     8581 tgccagtttt tatgagggag gcccgaagtc gactttatcg ctgtgacaca tactttctgg
     8641 gcaaaacgat tgccgaatta ccgctttttc tcacagtgcc actggtcttc acggcgattg
     8701 cctatccgat gatcggactg cgggccggag tgctgcactt cttcaactgc ctggcgctgg
     8761 tcactctggt ggccaatgtg tcaacgtcct tcggatatct aatatcctgc gccagctcct
     8821 cgacctcgat ggcgctgtct gtgggtccgc cggttatcat accattcctg ctctttggcg
     8881 gcttcttctt gaactcgggc tcggtgccag tatacctcaa atggttgtcg tacctctcat
     8941 ggttccgtta cgccaacgag ggtctgctga ttaaccaatg ggcggacgtg gagccgggcg
     9001 aaattagctg cacatcgtcg aacaccacgt gccccagttc gggcaaggtc atcctggaga
     9061 cgcttaactt ctccgccgcc gatctgccgc tggactacgt gggtctggcc attctcatcg
     9121 tgagcttccg ggtgctcgca tatctggctc taagacttcg ggcccgacgc aaggagtagc
     9181 cgacatatat ccgaaataac tgcttgtttt tttttttacc attattacca tcgtgtttac
     9241 tgtttattgc cccctcaaaa agctaatgta attatatttg tgccaataaa aacaagatat
     9301 gacctataga atacaagtat ttccccttcg aacatcccca caagtagact ttggatttgt
     9361 cttctaacca aaagacttac acacctgcat accttacatc aaaaactcgt ttatcgctac
     9421 ataaaacacc gggatatatt ttttatatac atacttttca aatcgcgcgc cctcttcata
     9481 attcacctcc accacaccac gtttcgtagt tgctctttcg ctgtctccca cccgctctcc
     9541 gcaacacatt caccttttgt tcgacgacct tggagcgact gtcgttagtt ccgcgcgatt
     9601 cggttcgctc aaatggttcc gagtggttca tttcgtctca atagaaatta gtaataaata
     9661 tttgtatgta caatttattt gctccaatat atttgtatat atttccctca cagctatatt
     9721 tattctaatt taatattatg actttttaag gtaatttttt gtgacctgtt cggagtgatt
     9781 agcgttacaa tttgaactga aagtgacatc cagtgtttgt tccttgtgta gatgcatctc
     9841 aaaaaaatgg tgggcataat agtgttgttt atatatatca aaaataacaa ctataataat
     9901 aagaatacat ttaatttaga aaatgcttgg atttcactgg aactagaatt aattcggctg
     9961 ctgctctaaa cgacgcattt cgtactccaa agtacgaatt ttttccctca agctcttatt
    10021 ttcattaaac aatgaacagg acctaacgca cagtcacgtt attgtttaca taaatgattt
    10081 tttttactat tcaaacttac tctgtttgtg tactcccact ggtatagcct tcttttatct
    10141 tttctggttc aggctctatc actttactag gtacggcatc tgcgttgagt cgcctccttt
    10201 taaatgtctg accttttgca ggtgcagcct tccactgcga atcattaaag tgggtatcac
    10261 aaatttggga gttttcacca aggctgcacc caaggctctg ctcccacaat tttctcttaa
    10321 tagcacactt cggcacgtga attaatttta ctccagtcac agctttgcag caaaatttgc
    10381 aatatttcat ttttttttat tccacgtaag ggttaatgtt ttcaaaaaaa aattcgtccg
    10441 cacacaacct ttcctctcaa caagcaaacg tgcactgaat ttaagtgtat acttcggtaa
    10501 gcttcggcta tcgacgggac caccttatgt tatttcatca tg
//
