LOCUS       sequence-6728-ex       10648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Ahmad K, Henikoff S
  TITLE     The histone variant H3.3 marks active chromatin by 
            replication-independent nucleosome assembly.
  JOURNAL   Mol Cell 2002 Jun;9(6):1191-200.
  PUBMED    12086617
REFERENCE   2  (bases 1 to 10648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell"; 
            date: "2002-06"; volume: "9(6)"; pages: "1191-200"
COMMENT     Sequence Label: HS-H3-GFP
FEATURES             Location/Qualifiers
     source          1..10648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(236..824)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(316..335)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(995..1786,1787..1855))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1618..1637
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1856..1960)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2028..2046
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2084..2106)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2206..2225
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2762..2792,2793..2994)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3039..3133
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3152..3390
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     promoter        3615..3853
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             4318..5034
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4348..4376)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4971..4991
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     intron          5124..5189
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5319..5339
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5764..5898
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5801..5820)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5855..5874
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5925..5943)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6403..6474,6869..7142,7217..7871,7933..8248,8469..8600,
                     8671..9285)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(10063..10617,10618..10648)
                     /label=P element 5' end
ORIGIN
        1 gggccagacc cacgtagtcc agcggcagat cggcggcgga gaagttaagc gtctccagga
       61 tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt gcagctaatt tcgcccggct
      121 ccacgtccgc ccattggtta atcagcagac cctcgttggc gtaacggaac catgagaggt
      181 acgacaacca tttgaggtat actggcaccg agcccgagtt caagaagaag gcgtttttcc
      241 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      301 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      361 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      421 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      481 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      541 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      601 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      661 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      721 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      781 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      841 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
      901 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
      961 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     1021 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     1081 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     1141 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     1201 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     1261 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     1321 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     1381 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     1441 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     1501 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     1561 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     1621 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1681 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1741 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1801 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     1861 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1921 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     1981 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     2041 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     2101 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     2161 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     2221 ttgtactgag agtgcaccat atgcggtgtg aaataccgca ccgaatcgcg cggaactaac
     2281 gacagtcgct ccaaggtcgt cgaacaaaag gtgaatgtgt tgcggagagc gggtgggaga
     2341 cagcgaaaga gcaactacga aacgtggtgt ggtggaggtg aattatgaag agggcgcgcg
     2401 atttgaaaag tatgtatata aaaaatatat cccggtgttt tatgtagcga taaacgagtt
     2461 tttgatgtaa ggtatgcagg tgtgtaagtc ttttggttag aagacaaatc caaagtctac
     2521 ttgtggggat gttcgaaggg gaaatacttg tattctatag gtcatatctt gtttttattg
     2581 gcacaaatat aattacatta gctttttgag ggggcaataa acagtaaaca cgatggtaat
     2641 aatggtaaaa aaaaaaacaa gcagttattt cggatatatg tcggctactc cttgcgtcgg
     2701 gcccgaagtc ttagagccag atatgcgagc acccggaagc tcacgatgag aatggccaga
     2761 ccatgatgaa ataacataag gtggtcccgt cggcaagaga catccactta acgtatgctt
     2821 gcaataagtg cgagtgaaag gaatagtatt ctgagtgtcg tattgagtct gagtgagaca
     2881 gcgatatgat tgttgattaa cccttagcat gtccgtgggg tttgaattaa ctcataatat
     2941 taattagacg aaattatttt taaagtttta tttttaataa tttgcgagta cgcaaagctt
     3001 ctgcatgagc tcggatccaa gcttgcatgc ctgcaggtcg gagtactgtc ctccgagcgg
     3061 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
     3121 actgtcctcc gagcggagac tctagcgagc gccggagtat aaatagaggc gcttcgtcta
     3181 cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca
     3241 aataaacaag cgcagctgaa caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa
     3301 tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa
     3361 gtaattattg aatacaagaa gagaactctg aatagggaat tgggaattcc aaaacaaact
     3421 ggttattgtg gtaggtcatt tgtttggcag aaagaaaact cgagaaattt ctctggccgt
     3481 tattcgttat tctctctttt ctttttgggt ctctccctct ctgcactaat gctctctcac
     3541 tctgtcacac agtaaacggc atactgctct cgttggttcg agagagcgcg cctcgaatgt
     3601 tcgcgaaaag agcgccggag tataaataga ggcgcttcgt ctacggagcg acaattcaat
     3661 tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct
     3721 gaacaagcta aacaatctgc agtaaagtgc aagttaaagt gaatcaatta aaagtaacca
     3781 gcaaccaagt aaatcaactg caactactga aatctgccaa gaagtaatta ttgaatacaa
     3841 gaagagaact ctgggggatc tgatcaagag acaggatgag gtctagagga gatggctcgt
     3901 accaagcaaa ctgctcgcaa atcgactggt ggaaaggcgc cacgcaaaca actggctact
     3961 aaggccgctc gcaagagtgc tccagccacc ggaggtgtga agaagcccca ccgctatcgc
     4021 cctggaaccg tggccttgcg tgaaattcgt cgctaccaaa agagcaccga gcttctaatc
     4081 cgcaagctgc ctttccagcg tctggtgcgt gaaatcgctc aggactttaa gacggacttg
     4141 cgattccaga gctcggcggt tatggctctg caggaagcta gcgaagccta cctggttggt
     4201 ctcttcgaag ataccaactt gtgtgccatt catgccaagc gtatcaccat aatgcccaaa
     4261 gacatccagt tagcgcgacg cattcgcggc gagcgtgctc agcggccggt cgcaacaatg
     4321 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     4381 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     4441 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
     4501 actactttca cttatggtgt tcaatgcttt tcaagatacc cagatcatat gaagcggcac
     4561 gacttcttca agagcgccat gcctgaggga tacgtgcagg agaggaccat cttcttcaag
     4621 gacgacggga actacaagac acgtgctgaa gtcaagtttg agggagacac cctcgtcaac
     4681 aggatcgagc ttaagggaat cgatttcaag gaggacggaa acatcctcgg ccacaagttg
     4741 gaatacaact acaactccca caacgtatac atcatggccg acaagcaaaa gaacggcatc
     4801 aaagccaact tcaagacccg ccacaacatc gaagacggcg gcgtgcaact cgctgatcat
     4861 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     4921 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     4981 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataaagatcc
     5041 actagaggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     5101 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     5161 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     5221 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
     5281 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
     5341 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
     5401 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
     5461 agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata
     5521 acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc
     5581 aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt
     5641 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct
     5701 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     5761 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5821 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5881 tcttatcatg tctggatcgg atccactaga aggccttagt atgtatgtaa gttaataaaa
     5941 cccttttttg gagaatgtag atttaaaaaa acatattttt tttttatttt ttactgcact
     6001 ggacatcatt gaacttatct gatcagtttt aaatttactt cgatccaagg gtatttgaag
     6061 taccaggttc tttcgattac ctctcactca aaatgacatt ccactcaaag tcagcgctgt
     6121 ttgcctcctt ctctgtccac agaaatatcg ccgtctcttt cgccgctgcg tccgctatct
     6181 ctttcgccac cgtttgtagc gttacctagc gtcaatgtcc gccttcagtt gcactttgtc
     6241 agcggtttcg tgacgaagct ccaagcggtt tacgccatca attaaacaca aagtgctgtg
     6301 ccaaaactcc tctcgcttct tatttttgtt tgttttttga gtgattgggg tggtgattgg
     6361 ttttgggtgg gtaagcaggg gaaagtgtga aaaatcccgg caatgggcca agaggatcag
     6421 gagctattaa ttcgcggagg cagcaaacac ccatctgccg agcatctgaa caatgtgagt
     6481 agtacatgtg catacatctt aagttcactt gatctatagg aactgcgatt gcaacatcaa
     6541 attgtctgcg gcgtgagaac tgcgacccac aaaaatccca aaccgcaatc gcacaaacaa
     6601 atagtgacac gaaacagatt attctggtag ctgtgctcgc tatataagac aatttttaag
     6661 atcatatcat gatcaagaca tctaaaggca ttcattttcg actacattct tttttacaaa
     6721 aaatataaca accagatatt ttaagctgat cctagatgca caaaaaataa ataaaagtat
     6781 aaacctactt cgtaggatac ttcgttttgt tcggggttag atgagcataa cgcttgtagt
     6841 tgatatttga gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat
     6901 taaccagggc ttcgggcagg ccaaaaacta cggcacgctc ctgccaccca gtccgccgga
     6961 ggactccggt tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat
     7021 ggacatcttt ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac
     7081 acgcggacta ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa
     7141 cggtgagttt ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta
     7201 ccaatttgaa actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc
     7261 agttccggtg ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc
     7321 atccaagtat cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag
     7381 atgcaggcca ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc
     7441 agggaacacc tgattttcca ggccatggtg cggatgccac gacatctgac ctatcggcag
     7501 cgagtggccc gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg
     7561 atcatcggtg tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca
     7621 ttcgcctccg aggcactaac cgatccgccg cttctgatct gcgatgagcc cacctccgga
     7681 ctggactcat ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc
     7741 aagaccgtca tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag
     7801 atccttctga tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac
     7861 ttcttttcct agtgagttcg atgtgtttat taagggtatc tagcattaca ttacatctca
     7921 actcctatcc agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt
     7981 acaggtgttg gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat
     8041 atgcgacaat tttgctatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa
     8101 aaatttggag aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt
     8161 catgcagttc cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct
     8221 cgtaaaagtg cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa
     8281 cgcttacaat tcttggaaac aaattcgcta gattttagtt agaattgcct gattccacac
     8341 ccttcttagt ttttttcaat gagatgtata gtttatagtt ttgcagaaaa taaataaatt
     8401 tcatttaact cgcgaacatg ttgaagatat gaatattaat gagatgcgag taacatttta
     8461 atttgcagat ggttgccatc ttgattggcc tcatcttttt gggccaacaa ctcacgcaag
     8521 tgggcgtgat gaatatcaac ggagccatct tcctcttcct gaccaacatg acctttcaaa
     8581 acgtctttgc cacgataaat gtaagtcttg tttagaatac atttgcatat taataattta
     8641 ctaactttct aatgaatcga ttcgatttag gtgttcacct cagagctgcc agtttttatg
     8701 agggaggccc gaagtcgact ttatcgctgt gacacatact ttctgggcaa aacgattgcc
     8761 gaattaccgc tttttctcac agtgccactg gtcttcacgg cgattgccta tccgatgatc
     8821 ggactgcggg ccggagtgct gcacttcttc aactgcctgg cgctggtcac tctggtggcc
     8881 aatgtgtcaa cgtccttcgg atatctaata tcctgcgcca gctcctcgac ctcgatggcg
     8941 ctgtctgtgg gtccgccggt tatcatacca ttcctgctct ttggcggctt cttcttgaac
     9001 tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt ccgttacgcc
     9061 aacgagggtc tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat tagctgcaca
     9121 tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc tggagacgct taacttctcc
     9181 gccgccgatc tgccgctgga ctacgtgggt ctggccattc tcatcgtgag cttccgggtg
     9241 ctcgcatatc tggctctaag acttcgggcc cgacgcaagg agtagccgac atatatccga
     9301 aataactgct tgtttttttt tttaccatta ttaccatcgt gtttactgtt tattgccccc
     9361 tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca agatatgacc tatagaatac
     9421 aagtatttcc ccttcgaaca tccccacaag tagactttgg atttgtcttc taaccaaaag
     9481 acttacacac ctgcatacct tacatcaaaa actcgtttat cgctacataa aacaccggga
     9541 tatatttttt atatacatac ttttcaaatc gcgcgccctc ttcataattc acctccacca
     9601 caccacgttt cgtagttgct ctttcgctgt ctcccacccg ctctccgcaa cacattcacc
     9661 ttttgttcga cgaccttgga gcgactgtcg ttagttccgc gcgattcggt tcgctcaaat
     9721 ggttccgagt ggttcatttc gtctcaatag aaattagtaa taaatatttg tatgtacaat
     9781 ttatttgctc caatatattt gtatatattt ccctcacagc tatatttatt ctaatttaat
     9841 attatgactt tttaaggtaa ttttttgtga cctgttcgga gtgattagcg ttacaatttg
     9901 aactgaaagt gacatccagt gtttgttcct tgtgtagatg catctcaaaa aaatggtggg
     9961 cataatagtg ttgtttatat atatcaaaaa taacaactat aataataaga atacatttaa
    10021 tttagaaaat gcttggattt cactggaact agaattaatt cggctgctgc tctaaacgac
    10081 gcatttcgta ctccaaagta cgaatttttt ccctcaagct cttattttca ttaaacaatg
    10141 aacaggacct aacgcacagt cacgttattg tttacataaa tgattttttt tactattcaa
    10201 acttactctg tttgtgtact cccactggta tagccttctt ttatcttttc tggttcaggc
    10261 tctatcactt tactaggtac ggcatctgcg ttgagtcgcc tccttttaaa tgtctgacct
    10321 tttgcaggtg cagccttcca ctgcgaatca ttaaagtggg tatcacaaat ttgggagttt
    10381 tcaccaaggc tgcacccaag gctctgctcc cacaattttc tcttaatagc acacttcggc
    10441 acgtgaatta attttactcc agtcacagct ttgcagcaaa atttgcaata tttcattttt
    10501 ttttattcca cgtaagggtt aatgttttca aaaaaaaatt cgtccgcaca caacctttcc
    10561 tctcaacaag caaacgtgca ctgaatttaa gtgtatactt cggtaagctt cggctatcga
    10621 cgggaccacc ttatgttatt tcatcatg
//
