LOCUS       sequence-6729-ex       10545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10545)
  AUTHORS   Ahmad K, Henikoff S
  TITLE     The histone variant H3.3 marks active chromatin by 
            replication-independent nucleosome assembly.
  JOURNAL   Mol Cell 2002 Jun;9(6):1191-200.
  PUBMED    12086617
REFERENCE   2  (bases 1 to 10545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell"; 
            date: "2002-06"; volume: "9(6)"; pages: "1191-200"
COMMENT     Sequence Label: HS-H4-GFP
FEATURES             Location/Qualifiers
     source          1..10545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(236..824)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(316..335)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(995..1786,1787..1855))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1618..1637
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1856..1960)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2028..2046
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2084..2106)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2206..2225
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2762..2792,2793..2994)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3039..3133
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3152..3390
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     promoter        3615..3853
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             4215..4931
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4245..4273)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4868..4888
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     intron          5021..5086
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5216..5236
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5661..5795
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5698..5717)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5752..5771
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5822..5840)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6300..6371,6766..7039,7114..7768,7830..8145,8366..8497,
                     8568..9182)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9960..10514,10515..10545)
                     /label=P element 5' end
ORIGIN
        1 gggccagacc cacgtagtcc agcggcagat cggcggcgga gaagttaagc gtctccagga
       61 tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt gcagctaatt tcgcccggct
      121 ccacgtccgc ccattggtta atcagcagac cctcgttggc gtaacggaac catgagaggt
      181 acgacaacca tttgaggtat actggcaccg agcccgagtt caagaagaag gcgtttttcc
      241 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      301 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      361 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      421 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      481 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      541 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      601 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      661 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      721 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      781 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      841 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
      901 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
      961 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     1021 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     1081 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     1141 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     1201 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     1261 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     1321 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     1381 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     1441 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     1501 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     1561 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     1621 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1681 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1741 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1801 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     1861 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1921 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     1981 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     2041 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     2101 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     2161 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     2221 ttgtactgag agtgcaccat atgcggtgtg aaataccgca ccgaatcgcg cggaactaac
     2281 gacagtcgct ccaaggtcgt cgaacaaaag gtgaatgtgt tgcggagagc gggtgggaga
     2341 cagcgaaaga gcaactacga aacgtggtgt ggtggaggtg aattatgaag agggcgcgcg
     2401 atttgaaaag tatgtatata aaaaatatat cccggtgttt tatgtagcga taaacgagtt
     2461 tttgatgtaa ggtatgcagg tgtgtaagtc ttttggttag aagacaaatc caaagtctac
     2521 ttgtggggat gttcgaaggg gaaatacttg tattctatag gtcatatctt gtttttattg
     2581 gcacaaatat aattacatta gctttttgag ggggcaataa acagtaaaca cgatggtaat
     2641 aatggtaaaa aaaaaaacaa gcagttattt cggatatatg tcggctactc cttgcgtcgg
     2701 gcccgaagtc ttagagccag atatgcgagc acccggaagc tcacgatgag aatggccaga
     2761 ccatgatgaa ataacataag gtggtcccgt cggcaagaga catccactta acgtatgctt
     2821 gcaataagtg cgagtgaaag gaatagtatt ctgagtgtcg tattgagtct gagtgagaca
     2881 gcgatatgat tgttgattaa cccttagcat gtccgtgggg tttgaattaa ctcataatat
     2941 taattagacg aaattatttt taaagtttta tttttaataa tttgcgagta cgcaaagctt
     3001 ctgcatgagc tcggatccaa gcttgcatgc ctgcaggtcg gagtactgtc ctccgagcgg
     3061 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
     3121 actgtcctcc gagcggagac tctagcgagc gccggagtat aaatagaggc gcttcgtcta
     3181 cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca
     3241 aataaacaag cgcagctgaa caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa
     3301 tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa
     3361 gtaattattg aatacaagaa gagaactctg aatagggaat tgggaattcc aaaacaaact
     3421 ggttattgtg gtaggtcatt tgtttggcag aaagaaaact cgagaaattt ctctggccgt
     3481 tattcgttat tctctctttt ctttttgggt ctctccctct ctgcactaat gctctctcac
     3541 tctgtcacac agtaaacggc atactgctct cgttggttcg agagagcgcg cctcgaatgt
     3601 tcgcgaaaag agcgccggag tataaataga ggcgcttcgt ctacggagcg acaattcaat
     3661 tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct
     3721 gaacaagcta aacaatctgc agtaaagtgc aagttaaagt gaatcaatta aaagtaacca
     3781 gcaaccaagt aaatcaactg caactactga aatctgccaa gaagtaatta ttgaatacaa
     3841 gaagagaact ctgggggatc tgatcaagag acaggatgag gtctagaatg actggtcgtg
     3901 gtaaaggagg caaaggcttg ggaaagggtg gcgccaagcg tcatcgcaaa gtgctgcgtg
     3961 ataacatcca aggtatcacg aagcctgcta tccgccgttt ggcccgtcga ggcggtgtga
     4021 agcgcatatc tggactcata tacgaggaaa cgcgtggcgt tctgaaggtt ttcttggaga
     4081 acgtaattcg tgatgccgtg acctacacgg aacacgccaa gaggaagaca gttacagcca
     4141 tggatgttgt gtacgctctg aagaggcaag gccgcaccct ctacggattt ggcggttcac
     4201 ggccggtcgc aacaatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
     4261 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     4321 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     4381 catggccaac acttgtcact actttcactt atggtgttca atgcttttca agatacccag
     4441 atcatatgaa gcggcacgac ttcttcaaga gcgccatgcc tgagggatac gtgcaggaga
     4501 ggaccatctt cttcaaggac gacgggaact acaagacacg tgctgaagtc aagtttgagg
     4561 gagacaccct cgtcaacagg atcgagctta agggaatcga tttcaaggag gacggaaaca
     4621 tcctcggcca caagttggaa tacaactaca actcccacaa cgtatacatc atggccgaca
     4681 agcaaaagaa cggcatcaaa gccaacttca agacccgcca caacatcgaa gacggcggcg
     4741 tgcaactcgc tgatcattat caacaaaata ctccaattgg cgatggccct gtccttttac
     4801 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
     4861 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
     4921 tatacaaata aagatccact agaggatctt tgtgaaggaa ccttacttct gtggtgtgac
     4981 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     5041 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     5101 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
     5161 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
     5221 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
     5281 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
     5341 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
     5401 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
     5461 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
     5521 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
     5581 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     5641 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     5701 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5761 ccaaactcat caatgtatct tatcatgtct ggatcggatc cactagaagg ccttagtatg
     5821 tatgtaagtt aataaaaccc ttttttggag aatgtagatt taaaaaaaca tatttttttt
     5881 ttatttttta ctgcactgga catcattgaa cttatctgat cagttttaaa tttacttcga
     5941 tccaagggta tttgaagtac caggttcttt cgattacctc tcactcaaaa tgacattcca
     6001 ctcaaagtca gcgctgtttg cctccttctc tgtccacaga aatatcgccg tctctttcgc
     6061 cgctgcgtcc gctatctctt tcgccaccgt ttgtagcgtt acctagcgtc aatgtccgcc
     6121 ttcagttgca ctttgtcagc ggtttcgtga cgaagctcca agcggtttac gccatcaatt
     6181 aaacacaaag tgctgtgcca aaactcctct cgcttcttat ttttgtttgt tttttgagtg
     6241 attggggtgg tgattggttt tgggtgggta agcaggggaa agtgtgaaaa atcccggcaa
     6301 tgggccaaga ggatcaggag ctattaattc gcggaggcag caaacaccca tctgccgagc
     6361 atctgaacaa tgtgagtagt acatgtgcat acatcttaag ttcacttgat ctataggaac
     6421 tgcgattgca acatcaaatt gtctgcggcg tgagaactgc gacccacaaa aatcccaaac
     6481 cgcaatcgca caaacaaata gtgacacgaa acagattatt ctggtagctg tgctcgctat
     6541 ataagacaat ttttaagatc atatcatgat caagacatct aaaggcattc attttcgact
     6601 acattctttt ttacaaaaaa tataacaacc agatatttta agctgatcct agatgcacaa
     6661 aaaataaata aaagtataaa cctacttcgt aggatacttc gttttgttcg gggttagatg
     6721 agcataacgc ttgtagttga tatttgagat cccctatcat tgcagggtga cagcggagcg
     6781 gcttcgcaga gctgcattaa ccagggcttc gggcaggcca aaaactacgg cacgctcctg
     6841 ccacccagtc cgccggagga ctccggttca gggagcggcc aactagccga gaacctcacc
     6901 tatgcctggc acaatatgga catctttggg gcggtcaatc agccgggctc cggatggcgg
     6961 cagctggtca accggacacg cggactattc tgcaacgagc gacacatacc ggcgcccagg
     7021 aaacatttgc tcaagaacgg tgagtttcta ttcgcagtcg gctgatctgt gtgaaatctt
     7081 aataaagggt ccaattacca atttgaaact cagtttgcgg cgtggcctat ccgggcgaac
     7141 ttttggccgt gatgggcagt tccggtgccg gaaagacgac cctgctgaat gcccttgcct
     7201 ttcgatcgcc gcagggcatc caagtatcgc catccgggat gcgactgctc aatggccaac
     7261 ctgtggacgc caaggagatg caggccaggt gcgcctatgt ccagcaggat gacctcttta
     7321 tcggctccct aacggccagg gaacacctga ttttccaggc catggtgcgg atgccacgac
     7381 atctgaccta tcggcagcga gtggcccgcg tggatcaggt gatccaggag ctttcgctca
     7441 gcaaatgtca gcacacgatc atcggtgtgc ccggcagggt gaaaggtctg tccggcggag
     7501 aaaggaagcg tctggcattc gcctccgagg cactaaccga tccgccgctt ctgatctgcg
     7561 atgagcccac ctccggactg gactcattta ccgcccacag cgtcgtccag gtgctgaaga
     7621 agctgtcgca gaagggcaag accgtcatcc tgaccattca tcagccgtct tccgagctgt
     7681 ttgagctctt tgacaagatc cttctgatgg ccgagggcag ggtagctttc ttgggcactc
     7741 ccagcgaagc cgtcgacttc ttttcctagt gagttcgatg tgtttattaa gggtatctag
     7801 cattacatta catctcaact cctatccagc gtgggtgccc agtgtcctac caactacaat
     7861 ccggcggact tttacgtaca ggtgttggcc gttgtgcccg gacgggagat cgagtcccgt
     7921 gatcggatcg ccaagatatg cgacaatttt gctattagca aagtagcccg ggatatggag
     7981 cagttgttgg ccaccaaaaa tttggagaag ccactggagc agccggagaa tgggtacacc
     8041 tacaaggcca cctggttcat gcagttccgg gcggtcctgt ggcgatcctg gctgtcggtg
     8101 ctcaaggaac cactcctcgt aaaagtgcga cttattcaga caacggtgag tggttccagt
     8161 ggaaacaaat gatataacgc ttacaattct tggaaacaaa ttcgctagat tttagttaga
     8221 attgcctgat tccacaccct tcttagtttt tttcaatgag atgtatagtt tatagttttg
     8281 cagaaaataa ataaatttca tttaactcgc gaacatgttg aagatatgaa tattaatgag
     8341 atgcgagtaa cattttaatt tgcagatggt tgccatcttg attggcctca tctttttggg
     8401 ccaacaactc acgcaagtgg gcgtgatgaa tatcaacgga gccatcttcc tcttcctgac
     8461 caacatgacc tttcaaaacg tctttgccac gataaatgta agtcttgttt agaatacatt
     8521 tgcatattaa taatttacta actttctaat gaatcgattc gatttaggtg ttcacctcag
     8581 agctgccagt ttttatgagg gaggcccgaa gtcgacttta tcgctgtgac acatactttc
     8641 tgggcaaaac gattgccgaa ttaccgcttt ttctcacagt gccactggtc ttcacggcga
     8701 ttgcctatcc gatgatcgga ctgcgggccg gagtgctgca cttcttcaac tgcctggcgc
     8761 tggtcactct ggtggccaat gtgtcaacgt ccttcggata tctaatatcc tgcgccagct
     8821 cctcgacctc gatggcgctg tctgtgggtc cgccggttat cataccattc ctgctctttg
     8881 gcggcttctt cttgaactcg ggctcggtgc cagtatacct caaatggttg tcgtacctct
     8941 catggttccg ttacgccaac gagggtctgc tgattaacca atgggcggac gtggagccgg
     9001 gcgaaattag ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag gtcatcctgg
     9061 agacgcttaa cttctccgcc gccgatctgc cgctggacta cgtgggtctg gccattctca
     9121 tcgtgagctt ccgggtgctc gcatatctgg ctctaagact tcgggcccga cgcaaggagt
     9181 agccgacata tatccgaaat aactgcttgt tttttttttt accattatta ccatcgtgtt
     9241 tactgtttat tgccccctca aaaagctaat gtaattatat ttgtgccaat aaaaacaaga
     9301 tatgacctat agaatacaag tatttcccct tcgaacatcc ccacaagtag actttggatt
     9361 tgtcttctaa ccaaaagact tacacacctg cataccttac atcaaaaact cgtttatcgc
     9421 tacataaaac accgggatat attttttata tacatacttt tcaaatcgcg cgccctcttc
     9481 ataattcacc tccaccacac cacgtttcgt agttgctctt tcgctgtctc ccacccgctc
     9541 tccgcaacac attcaccttt tgttcgacga ccttggagcg actgtcgtta gttccgcgcg
     9601 attcggttcg ctcaaatggt tccgagtggt tcatttcgtc tcaatagaaa ttagtaataa
     9661 atatttgtat gtacaattta tttgctccaa tatatttgta tatatttccc tcacagctat
     9721 atttattcta atttaatatt atgacttttt aaggtaattt tttgtgacct gttcggagtg
     9781 attagcgtta caatttgaac tgaaagtgac atccagtgtt tgttccttgt gtagatgcat
     9841 ctcaaaaaaa tggtgggcat aatagtgttg tttatatata tcaaaaataa caactataat
     9901 aataagaata catttaattt agaaaatgct tggatttcac tggaactaga attaattcgg
     9961 ctgctgctct aaacgacgca tttcgtactc caaagtacga attttttccc tcaagctctt
    10021 attttcatta aacaatgaac aggacctaac gcacagtcac gttattgttt acataaatga
    10081 ttttttttac tattcaaact tactctgttt gtgtactccc actggtatag ccttctttta
    10141 tcttttctgg ttcaggctct atcactttac taggtacggc atctgcgttg agtcgcctcc
    10201 ttttaaatgt ctgacctttt gcaggtgcag ccttccactg cgaatcatta aagtgggtat
    10261 cacaaatttg ggagttttca ccaaggctgc acccaaggct ctgctcccac aattttctct
    10321 taatagcaca cttcggcacg tgaattaatt ttactccagt cacagctttg cagcaaaatt
    10381 tgcaatattt catttttttt tattccacgt aagggttaat gttttcaaaa aaaaattcgt
    10441 ccgcacacaa cctttcctct caacaagcaa acgtgcactg aatttaagtg tatacttcgg
    10501 taagcttcgg ctatcgacgg gaccacctta tgttatttca tcatg
//
