LOCUS       sequence-67322-e        2220 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR
  TITLE     Targeted gene disruption in somatic zebrafish cells using engineered
            TALENs.
  JOURNAL   Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934.
  PUBMED    21822241
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-08-5- 5"; volume: "29"; 
            issue: "8"; pages: "697-8"
COMMENT     Sequence Label: TAL3516
FEATURES             Location/Qualifiers
     source          1..2220
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac
       61 gatgacaaga tggcccccaa gaagaagagg aaggtgggca ttcaccgcgg ggtacctatg
      121 gtggacttga ggacactcgg ttattcgcaa cagcaacagg agaaaatcaa gcctaaggtc
      181 aggagcaccg tcgcgcaaca ccacgaggcg cttgtggggc atggcttcac tcatgcgcat
      241 attgtcgcgc tttcacagca ccctgcggcg cttgggacgg tggctgtcaa ataccaagat
      301 atgattgcgg ccctgcccga agccacgcac gaggcaattg taggggtcgg taaacagtgg
      361 tcgggagcgc gagcacttga ggcgctgctg actgtggcgg gtgagcttag ggggcctccg
      421 ctccagctcg acaccgggca gctgctgaag atcgcgaaga gagggggagt aacagcggta
      481 gaggcagtgc acgcctggcg caatgcgctc accggggccc ccttgaacct gaccccagac
      541 caggtagtcg caatcgcgaa caataatggg ggaaagcaag ccctggaaac cgtgcaaagg
      601 ttgttgccgg tcctttgtca agaccacggc cttacaccgg agcaagtcgt ggccattgca
      661 tcccacgacg gtggcaaaca ggctcttgag acggttcaga gacttctccc agttctctgt
      721 caagcccacg ggctgactcc cgatcaagtt gtagcgattg cgtccaacgg tggagggaaa
      781 caagcattgg agactgtcca acggctcctt cccgtgttgt gtcaagccca cggtttgacg
      841 cctgcacaag tggtcgccat cgcctcgaat ggcggcggta agcaggcgct ggaaacagta
      901 cagcgcctgc tgcctgtact gtgccaggat catggactga ccccagacca ggtagtcgca
      961 atcgcgtcaa acggaggggg aaagcaagcc ctggaaaccg tgcaaaggtt gttgccggtc
     1021 ctttgtcaag accacggcct tacaccggag caagtcgtgg ccattgcatc ccacgacggt
     1081 ggcaaacagg ctcttgagac ggttcagaga cttctcccag ttctctgtca agcccacggg
     1141 ctgactcccg atcaagttgt agcgattgcg tcgaacattg gagggaaaca agcattggag
     1201 actgtccaac ggctccttcc cgtgttgtgt caagcccacg gtttgacgcc tgcacaagtg
     1261 gtcgccatcg ccagccatga tggcggtaag caggcgctgg aaacagtaca gcgcctgctg
     1321 cctgtactgt gccaggatca tggactgacc ccagaccagg tagtcgcaat cgcgaacaat
     1381 aatgggggaa agcaagccct ggaaaccgtg caaaggttgt tgccggtcct ttgtcaagac
     1441 cacggcctta caccggagca agtcgtggcc attgcatccc acgacggtgg caaacaggct
     1501 cttgagacgg ttcagagact tctcccagtt ctctgtcaag cccacgggct gactcccgat
     1561 caagttgtag cgattgcgtc gaacattgga gggaaacaag cattggagac tgtccaacgg
     1621 ctccttcccg tgttgtgtca agcccacggt ttgacgcctg cacaagtggt cgccatcgcc
     1681 tcgaatggcg gcggtaagca ggcgctggaa acagtacagc gcctgctgcc tgtactgtgc
     1741 caggatcatg gactgacccc agaccaggta gtcgcaatcg cgtcaaacgg agggggaaag
     1801 caagccctgg aaaccgtgca aaggttgttg ccggtccttt gtcaagacca cggccttaca
     1861 ccggagcaag tcgtggccat tgcaaataat aacggtggca aacaggctct tgagacggtt
     1921 cagagacttc tcccagttct ctgtcaagcc cacgggctga ctcccgatca agttgtagcg
     1981 attgcgtcgc atgacggagg gaaacaagca ttggagactg tccaacggct ccttcccgtg
     2041 ttgtgtcaag cccacggttt gacgcctgca caagtggtcg ccatcgcctc caatattggc
     2101 ggtaagcagg cgctggaaac agtacagcgc ctgctgcctg tactgtgcca ggatcatgga
     2161 ctgacacccg aacaggtggt cgccattgct tcccacgacg gaggacggcc agccttggag
//
