LOCUS       sequence-67400-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral vector for expression of Reverse Tetracycline 
            transactivator 3G (rtTA3G)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  TITLE     pLenti CMV rtTA3G Blast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti CMV rtTA3G Blast (R980-M38-658)
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(31..78)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(126..455)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      140..275
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(194..213)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     440..460
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             617..628
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aggcttggcc atggtttagt tcctcacctt gtcgtattat actatgccga tatactatgc
       61 cgatgattaa ttgtcaacac gtgctgatca gatccgaaaa tggatataca agctcccggg
      121 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
      181 gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
      241 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
      301 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
      361 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
      421 gagcctgggg actttccaca ccctaactga cacacattcc acagaattaa ttccaaactc
      481 attactaacc ggatatctgc agaattccag gcggggaggc ggcccaaagg gagatccgac
      541 tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg
      601 aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa
      661 tccaggtggc aacacaggcg agcagccatg gaaaggacgt cagcttcccc gacaacacca
      721 cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga
      781 tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg
      841 tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac
      901 gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg
      961 agg
//
