LOCUS       sequence-67401-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  TITLE     Murray Lab pBEST plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBEST-p15a-OR2-OR1-Pr-UTR1-sigma28-T500
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             757..777
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttggtcgact ctcgagtggc gcctctagat tataacttac ccagtttagt gcgtaaccgt
       61 ttaatagcct ggctgtgtaa ctgactgacc cgcgattccc cgacctccag caccgcgcca
      121 atctctttga gattcagctc ttcctggtaa tagagggtta ataccagttt ttcgcgctcc
      181 ggcaacgttt cgatggcttc catcacccgc tggcgcagat tactgtccag tagttgttgt
      241 agcgggtttt ctcgctgatg atcatcagta accagttcga tgctatcgcc gtgctcttcg
      301 cgccactcat cgtaggagaa gagctggctg ttattggtgt cgagcaacat ttggcgataa
      361 tcggcaatat cgatccctaa acgttccgct acctcagttt ccgtggcgtt gcggccaagt
      421 tcctgctcca gttgccctat tgcctgtgcc acttcacgcg cgttgcgtcg cacgctgcgc
      481 ggcacccagt cacggctgcg aagttcatcc agcatagcgc cacggatacg ctgcactgcg
      541 taagttgtaa atgccgttcc ttgtagggcg tcatagcgtt cgacggcatt aagtaaccca
      601 atgccgcccg cctgtagcag atcgtcaagt tccacgctcg cgggcagtcg aacctgcagg
      661 cgcaatgctt cgtgacgcac cagcgggaca taacgctgcc acagcgagtg tttatccatt
      721 acaccttcag cggtatagag tgaattcatt tggatcctcc ttcttaaagt taaacaaaat
      781 tattgctagc tgcaaccatt atcaccgcca gaggtaaaat tgtcaacacg cacggtgtta
      841 gctcagcacg ttaccggac
//
