LOCUS       sequence-67407-e       18151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Pong ORF1 and transposase from rice to allow mPing 
            movement. Contains ORF1 and transposase driven by a native soybean 
            promoter. Contains mPing and BAR for basta selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18151)
  AUTHORS   Hancock CN, Zhang F, Floyd K, Richardson AO, Lafayette P, Tucker D, 
            Wessler SR, Parrott WA
  TITLE     The rice miniature inverted repeat transposable element mPing is an 
            effective insertional mutagen in soybean.
  JOURNAL   Plant Physiol. 2011 Oct;157(2):552-62. doi: 10.1104/pp.111.181206. 
            Epub 2011 Aug 15.
  PUBMED    21844309
REFERENCE   2  (bases 1 to 18151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.111.181206"; journalName: "Plant Physiol"; date: 
            "2011-10"; volume: "157"; issue: "2"; pages: "552-62"
COMMENT     Sequence Label: pPong_v2
FEATURES             Location/Qualifiers
     source          1..18151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     primer_bind     881..900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1134..1151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2454..2478
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      5497..6203
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     terminator      8649..9356
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        9885..10230
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     10173..10193
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             10251..10802
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      11128..11380
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(11389..11406)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11389..11405)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11398..11420)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(11545..11564)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11755..11776
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    12023..12047
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(13643..14791)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(15090..15884)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  16076..16843
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      17536..18067
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctaga cggacgcggc cgcgaattca ctagtgatta ccaatgacgt
     2761 agctttaggc cagtcacaat ggctagtgtc attgcacggc tacccaaaat attataccat
     2821 cttctctcaa atgaaatctt ttatgaaaca atccccacag tggaggggtt tcactttgac
     2881 gtttccaaga ctaagcaaag catttaattg atacaagttg ctgggatcat ttgtacccaa
     2941 aatccggcgc ggcgcgggag aatgcggagg tcgcacggcg gaggcggacg caagagatcc
     3001 ggtgaatgaa acgaatcggc ctcaacgggg gtttcactct gttaccgagg acttggaaac
     3061 gacgctgacg agtttcacca ggatgaaact ctttccttct ctctcatccc catttcatgc
     3121 aaataatcat tttttattca gtcttacccc tattaaatgt gcatgacaca ccagtgaaac
     3181 ccccattgtg actggcctta aacaatatgt taacgaatcg aattcccgcg gccgcgggcc
     3241 caatataaca acgacgtcgt aacagataaa gcgaagcttg aaggtgcatg tgactccgtc
     3301 aagattacga aaccgccaac taccacgcaa attgcaattc tcaatttcct agaaggactc
     3361 tccgaaaatg catccaatac caaatattac ccgtgtcata ggcaccaagt gacaccatac
     3421 atgaacacgc gtcacaatat gactggagaa gggttccaca ccttatgcta taaaacgccc
     3481 cacacccctc ctccttcctt cgcagttcaa ttccaatata ttccattctc tctgtgtatt
     3541 tccctacctc tcccttcaag gttagtcgat ttcttctgtt tttcttcttc gttctttcca
     3601 tgaattgtgt atgttctttg atcaatacga tgttgatttg attgtgtttt gtttggtttc
     3661 atcgatcttc aattttcata atcagattca gcttttatta tctttacaac aacgtcctta
     3721 atttgatgat tctttaatcg tagatttgct ctaattagag ctttttcatg tcagatccct
     3781 ttacaacaag ccttaattgt tgattcatta atcgtagatt agggcttttt tcattgatta
     3841 cttcagatcc gttaaacgta accatagatc agggcttttt catgaattac ttcagatccg
     3901 ttaaacaaca gccttatttt ttatacttct gtggtttttc aagaaattgt tcagatccgt
     3961 tgacaaaaag ccttattcgt tgattctata tcgtttttcg agagatattg ctcagatctg
     4021 ttagcaactg ccttgtttgt tgattctatt gccgtggatt agggtttttt ttcacgagat
     4081 tgcttcagat ccgtacttaa gattacgtaa tggattttga ttctgattta tctgtgattg
     4141 ttgactcgac aggtaccttc aaacctcgag atggatccac aaggaggtgg tggatcccgt
     4201 ctgagcgccg ccggcagagg agggaataag cgtgggggca agcagctggg cctgaagagg
     4261 tcgtcggcgc ctgctccatc accggcaaca gctcagccac cgctgcctgc aagttcccct
     4321 cctgaagctc catcgccggc aacagttcag ccgcctactc catcgtcaag tcctgctgtt
     4381 gctgccccca gttcatcccc tgctgtaccg atgtcaacca tgcccccatg gccaccgcaa
     4441 ggagcaggat ggggctctgt accccccaat tttgcttttc tgcaaggaaa ccaacaaggc
     4501 ccaagttcat ggttgtatcc tacagaaggc ttcgtaaatt ttctccaaca gaactgtctg
     4561 ccgcagccac aagaaggtga aaattttcac cttgttggtc agactaccaa cacaatgtct
     4621 actccaccac caacacccca agctgcagct aacaatacag tccaaattga tattcatgaa
     4681 gatgcaatca atgatgcaag tgctaaaaag agaagtttga gatattggac tcatgatgag
     4741 gaagagagat tggctagtgc ttggttgaat gcttctaaag atcccattca tgggaatgaa
     4801 aagaaaggtg atacgttttg gaaagaggtt actgatgagt tcaacagaaa agggaatggg
     4861 aagcgtacaa gggaaataaa tcaattgaag gttcattggt cacgcctcaa atcatcgatt
     4921 ggagaattca atgattactg gactaaggta actcaaatga atacaagcgg atatgacgat
     4981 gacatgctgg agaaggaggc acaacagatg tatgcaaata catttggaaa gccttttgca
     5041 cttgtgcatt ggtggaagat actgagaaaa gagcccaagt ggtgtgcaat gattgagaag
     5101 gacaaaaaca aggctgaagt ggttgatatt ccagatgaac aaaagcgtcc cattggtaga
     5161 gaagcagcac aagccgagcg caatggaaaa cgcaagaagg acagtatgtc agaaggaatt
     5221 gtcatcctag gggacaatat tgaaaaaatt atcaaagtga cgcaagatcg gaagctggag
     5281 cgtgagaagg tcactgaagc acagattcac atttcaaacg taaatttgaa ggcagcagaa
     5341 cagcaaaaag aagcaaagat gtttgaggta tacaattccc tgctcactca agatacaagt
     5401 aacatgtctg aagaacagaa ggctcgccga gacaaggcat tacaaaagct ggaggaaaag
     5461 ttatttgctg actaaactag ttctaatcta gatgtcctgc tttaatgaga tatgcgagac
     5521 gcctatgatc gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc
     5581 atgtgtagct cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt
     5641 actatcgtat ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt
     5701 atatgtacaa tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct
     5761 atgatagagc gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa
     5821 aaagcggcag aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa
     5881 aaggccccag gggctagtat ctacgacaca ccgagcggcg aactaataac gttcactgaa
     5941 gggaactccg gttccccgcc ggcgcgcatg ggtgagattc cttgaagttg agtattggcc
     6001 gtccgctcta ccgaaagtta cgggcaccat tcaacccggt ccagcacggc ggccgggtaa
     6061 ccgacttgct gccccgagaa ttatgcagca tttttttggt gtatgtgggc cccaaatgaa
     6121 gtgcaggtca aaccttgaca gtgacgacaa atcgttgggc gggtccaggg cgaattttgc
     6181 gacaacatgt cgaggctcag caggacctgc aggcatgcaa gctagtgaag aatcgaaggg
     6241 cccaatataa caacgacgtc gtaacagata aagcgaagct tgaaggtgca tgtgactccg
     6301 tcaagattac gaaaccgcca actaccacgc aaattgcaat tctcaatttc ctagaaggac
     6361 tctccgaaaa tgcatccaat accaaatatt acccgtgtca taggcaccaa gtgacaccat
     6421 acatgaacac gcgtcacaat atgactggag aagggttcca caccttatgc tataaaacgc
     6481 cccacacccc tcctccttcc ttcgcagttc aattccaata tattccattc tctctgtgta
     6541 tttccctacc tctcccttca aggttagtcg atttcttctg tttttcttct tcgttctttc
     6601 catgaattgt gtatgttctt tgatcaatac gatgttgatt tgattgtgtt ttgtttggtt
     6661 tcatcgatct tcaattttca taatcagatt cagcttttat tatctttaca acaacgtcct
     6721 taatttgatg attctttaat cgtagatttg ctctaattag agctttttca tgtcagatcc
     6781 ctttacaaca agccttaatt gttgattcat taatcgtaga ttagggcttt tttcattgat
     6841 tacttcagat ccgttaaacg taaccataga tcagggcttt ttcatgaatt acttcagatc
     6901 cgttaaacaa cagccttatt ttttatactt ctgtggtttt tcaagaaatt gttcagatcc
     6961 gttgacaaaa agccttattc gttgattcta tatcgttttt cgagagatat tgctcagatc
     7021 tgttagcaac tgccttgttt gttgattcta ttgccgtgga ttagggtttt ttttcacgag
     7081 attgcttcag atccgtactt aagattacgt aatggatttt gattctgatt tatctgtgat
     7141 tgttgactcg acaggtacct tcaaacggcg cgccatgcag agtttagcca tctctctact
     7201 cctctcagaa actcattccc tcttttctca tacgaagacc tcctcccttt tatctttact
     7261 gtttctctct tcttcaaaga tgtctgagca aaatactgat ggaagtcaag ttccagtgaa
     7321 cttgttggat gagttcctgg ctgaggatga gatcatagat gatcttctca ctgaagccac
     7381 ggtggtagta cagtccacta tagaaggtct tcaaaacgag gcttctgacc atcgacatca
     7441 tccgaggaag cacatcaaga ggccacgaga ggaagcacat cagcaactag tgaatgatta
     7501 cttttcagaa aatcctcttt acccttccaa aatttttcgt cgaagatttc gtatgtctag
     7561 gccacttttt cttcgcatcg ttgaggcatt aggccagtgg tcagtgtatt tcacacaaag
     7621 ggtggatgct gttaatcgga aaggactcag tccactgcaa aagtgtactg cagctattcg
     7681 ccagttggct actggtagtg gcgcagatga actagatgaa tatctgaaga taggagagac
     7741 tacagcaatg gaggcaatga agaattttgt caaaggtctt caagatgtgt ttggtgagag
     7801 gtatcttagg cgccccacca tggaagatac cgaacggctt ctccaacttg gtgagaaacg
     7861 tggttttcct ggaatgttcg gcagcattga ctgcatgcac tggcattggg aaagatgccc
     7921 agtagcatgg aagggtcagt tcactcgtgg agatcagaaa gtgccaaccc tgattcttga
     7981 ggctgtggca tcgcatgatc tttggatttg gcatgcattt tttggagcag cgggttccaa
     8041 caatgatatc aatgtattga accaatctac tgtatttatc aaggagctca aaggacaagc
     8101 tcctagagtc cagtacatgg taaatgggaa tcaatacaat actgggtatt ttcttgctga
     8161 tggaatctac cctgaatggg cagtgtttgt taagtcaata cgactcccaa acactgaaaa
     8221 ggagaaattg tatgcagata tgcaagaagg ggcaagaaaa gatatcgaga gagcctttgg
     8281 tgtattgcag cgaagatttt gcatcttaaa acgaccagct cgtctatatg atcgaggtgt
     8341 actgcgagat gttgttctag cttgcatcat acttcacaat atgatagttg aagatgagaa
     8401 ggaaaccaga attattgaag aagatgcaga tgcaaatgtg cctcctagtt catcaaccgt
     8461 tcaggaacct gagttctctc ctgaacagaa cacaccattt gatagagttt tagaaaaaga
     8521 tatttctatc cgagatcgag cggctcataa ccgacttaag aaagatttgg tggaacacat
     8581 ttggaataag tttggtggtg ctgcacatag aactggaaat tgattaatta acattctaat
     8641 ctagagtcct gctttaatga gatatgcgag acgcctatga tcgcatgata tttgctttca
     8701 attctgttgt gcacgttgta aaaaacctga gcatgtgtag ctcagatcct taccgccggt
     8761 ttcggttcat tctaatgaat atatcacccg ttactatcgt atttttatga ataatattct
     8821 ccgttcaatt tactgattgt accctactac ttatatgtac aatattaaaa tgaaaacaat
     8881 atattgtgct gaataggttt atagcgacat ctatgataga gcgccacaat aacaaacaat
     8941 tgcgttttat tattacaaat ccaattttaa aaaaagcggc agaaccggtc aaacctaaaa
     9001 gactgattac ataaatctta ttcaaatttc aaaagtgccc caggggctag tatctacgac
     9061 acaccgagcg gcgaactaat aacgttcact gaagggaact ccggttcccc gccggcgcgc
     9121 atgggtgaga ttccttgaag ttgagtattg gccgtccgct ctaccgaaag ttacgggcac
     9181 cattcaaccc ggtccagcac ggcggccggg taaccgactt gctgccccga gaattatgca
     9241 gcattttttt ggtgtatgtg ggccccaaat gaagtgcagg tcaaaccttg acagtgacga
     9301 caaatcgttg ggcgggtcca gggcgaattt tgcgacaaca tgtcgaggct cagcaggacc
     9361 tgcaggcatg caagatgtat tgcccctgca ggtccccaga ttagcctttt caatttcaga
     9421 aagaatgcta acccacagat ggttagagag gcttacgcag caggtctcat caagacgatc
     9481 tacccgagca ataatctcca ggaaatcaaa taccttccca agaaggttaa agatgcagtc
     9541 aaaagattca ggactaactg catcaagaac acagagaaag atatatttct caagatcaga
     9601 agtactattc cagtatggac gattcaaggc ttgcttcaca aaccaaggca agtaatagag
     9661 attggagtct ctaaaaaggt agttcccact gaatcaaagg ccatggagtc aaagattcaa
     9721 atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca gagtctctta
     9781 cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac acttgtctac
     9841 tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa
     9901 agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg
     9961 aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc
    10021 atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
    10081 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
    10141 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
    10201 taaggaagtt catttcattt ggagagaaca cgggggactc tagaggatcc atgagcccag
    10261 aacgacgccc ggccgacatc cgccgtgcca ccgaggcgga catgccggcg gtctgcacca
    10321 tcgtcaacca ctacatcgag acaagcacgg tcaacttccg taccgagccg caggaaccgc
    10381 aggagtggac ggacgacctc gtccgtctgc gggagcgcta tccctggctc gtcgccgagg
    10441 tggacggcga ggtcgccggc atcgcctacg cgggcccctg gaaggcacgc aacgcctacg
    10501 actggacggc cgagtcgacc gtgtacgtct ccccccgcca ccagcggacg ggactgggct
    10561 ccacgctcta cacccacctg ctgaagtccc tggaggcaca gggcttcaag agcgtggtcg
    10621 ctgtcatcgg gctgcccaac gacccgagcg tgcgcatgca cgaggcgctc ggatatgccc
    10681 cccgcggcat gctgcgggcg gccggcttca agcacgggaa ctggcatgac gtgggtttct
    10741 ggcagctgga cttcagcctg ccggtaccgc cccgtccggt cctgcccgtc accgagatct
    10801 gaacggagtg cgcgtggcat cgcccgagtt ggagctggta cgggaactca tcgaactcaa
    10861 ctggcatacc cgcaatggtg aggtggaacc gcggcggatc gcgtacgacc gtgcccagga
    10921 gggggtaccg agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag
    10981 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
    11041 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
    11101 agtcccgcaa ttagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta
    11161 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
    11221 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
    11281 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
    11341 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattcac tggccgtcgt
    11401 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
    11461 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
    11521 gttgcgcagc ctgaatggcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
    11581 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
    11641 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
    11701 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
    11761 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag
    11821 ggattttgcc gatttcggaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
    11881 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
    11941 cgtctcactg gtgaaaagaa aaaccacccc agtacattaa aaacgtccgc aatgtgttat
    12001 taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac
    12061 agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc
    12121 gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc
    12181 tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt
    12241 agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaaatat catctccctc
    12301 gcagagatcc gaattatcag ccttcttatt catttctcgc ttaaccgtga caggctgtcg
    12361 atcttgagaa ctatgccgac ataataggaa atcgctggat aaagccgctg aggaagctga
    12421 gtggcgctat ttctttagaa gtgaacgttg acgatatcaa ctcccctatc cattgctcac
    12481 cgaatggtac aggtcgggga cccgaagttc cgactgtcgg cctgatgcat ccccggctga
    12541 tcgaccccag atctggggct gagaaagccc agtaaggaaa caactgtagg ttcgagtcgc
    12601 gagatccccc ggaaccaaag gaagtaggtt aaacccgctc cgatcaggcc gagccacgcc
    12661 aggccgagaa cattggttcc tgtaggcatc gggattggcg gatcaaacac taaagctact
    12721 ggaacgagca gaagtcctcc ggccgccagt tgccaggcgg taaaggtgag cagaggcacg
    12781 ggaggttgcc acttgcgggt cagcacggtt ccgaacgcca tggaaaccgc ccccgccagg
    12841 cccgctgcga cgccgacagg atctagcgct gcgtttggtg tcaacaccaa cagcgccacg
    12901 cccgcagttc cgcaaatagc ccccaggacc gccatcaatc gtatcgggct acctagcaga
    12961 gcggcagaga tgaacacgac catcagcggc tgcacagcgc ctaccgtcgc cgcgaccccg
    13021 cccggcaggc ggtagaccga aataaacaac aagctccaga atagcgaaat attaagtgcg
    13081 ccgaggatga agatgcgcat ccaccagatt cccgttggaa tctgtcggac gatcatcacg
    13141 agcaataaac ccgccggcaa cgcccgcagc agcataccgg cgacccctcg gcctcgctgt
    13201 tcgggctcca cgaaaacgcc ggacagatgc gccttgtgag cgtccttggg gccgtcctcc
    13261 tgtttgaaga ccgacagccc aatgatctcg ccgtcgatgt aggcgccgaa tgccacggca
    13321 tctcgcaacc gttcagcgaa cgcctccatg ggctttttct cctcgtgctc gtaaacggac
    13381 ccgaacatct ctggagcttt cttcagggcc gacaatcgga tctcgcggaa atcctgcacg
    13441 tcggccgctc caagccgtcg aatctgagcc ttaatcacaa ttgtcaattt taatcctctg
    13501 tttatcggca gttcgtagag cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc
    13561 gtcgagcagt gcccgcttgt tcctgaaatg ccagtaaagc gctggctgct gaacccccag
    13621 ccggaactga ccccacaagg ccctagcgtt tgcaatgcac caggtcatca ttgacccagg
    13681 cgtgttccac caggccgctg cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc
    13741 acttcttcac gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc agcttgagcg
    13801 ggtacggctc ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct
    13861 tgcggtactt ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc acgacgattt
    13921 cctcgtcgat caggacctgg caacgggacg ttttcttgcc acggtccagg acgcggaagc
    13981 ggtgcagcag cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg
    14041 tcgcctgtag gcgcgacagg cattcctcgg ccttcgtgta ataccggcca ttgatcgacc
    14101 agcccaggtc ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc
    14161 gcttcgcgta ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga
    14221 cgccggtgta ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg
    14281 cctcgcgcgg gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg
    14341 gcatcgctcg catcgtgtcc ggccacggcg caatatcgaa caaggaaagc tgcatttcct
    14401 tgatctgctg cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg acctgttttg
    14461 ccaggtcctc gccggcggtt tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg
    14521 tcatcgactt cgccaaacct gccgcctcct gttcgagacg acgcgaacgc tccacggcgg
    14581 ccgatggcgc gggcagggca gggggagcca gttgcacgct gtcgcgctcg atcttggccg
    14641 tagcttgctg gaccatcgag ccgacggact ggaaggtttc gcggggcgca cgcatgacgg
    14701 tgcggcttgc gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc
    14761 tgtatgcctt ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt gccccgactc
    14821 acgccggggc aatgtgccct tattcctgat ttgacccgcc tggtgccttg gtgtccagat
    14881 aatccacctt atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact
    14941 tggtattccg aatcttgccc tgcacgaata ccagcgaccc cttgcccaaa tacttgccgt
    15001 gggcctcggc ctgagagcca aaacacttga tgcggaagaa gtcggtgcgc tcctgcttgt
    15061 cgccggcatc gttgcgccac atctaggtac taaaacaatt catccagtaa aatataatat
    15121 tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt
    15181 tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc
    15241 gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg
    15301 ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt
    15361 aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa
    15421 tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag
    15481 ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca
    15541 tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg
    15601 ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc
    15661 tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca
    15721 taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc
    15781 accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt
    15841 tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct
    15901 cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt
    15961 cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt
    16021 caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccacaa ttatgggtga
    16081 tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg
    16141 tgtctatcag ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca
    16201 ccgccggaca tcagcgctat ctctgctctc actgccgtaa aacatggcaa ctgcagttca
    16261 cttacaccgc ttctcaaccc ggtacgcacc agaaaatcat tgatatggcc atgaatggcg
    16321 ttggatgccg ggcaacagcc cgcattatgg gcgttggcct caacacgatt ttacgtcact
    16381 taaaaaactc aggccgcagt cggtaacctc gcgcatacag ccgggcagtg acgtcatcgt
    16441 ctgcgcggaa atggacgaac agtggggcta tgtcggggct aaatcgcgcc agcgctggct
    16501 gttttacgcg tatgacagtc tccggaagac ggttgttgcg cacgtattcg gtgaacgcac
    16561 tatggcgacg ctggggcgtc ttatgagcct gctgtcaccc tttgacgtgg tgatatggat
    16621 gacggatggc tggccgctgt atgaatcccg cctgaaggga aagctgcacg taatcagcaa
    16681 gcgatatacg cagcgaattg agcggcataa cctgaatctg aggcagcacc tggcacggct
    16741 gggacggaag tcgctgtcgt tctcaaaatc ggtggagctg catgacaaag tcatcgggca
    16801 ttatctgaac ataaaacact atcaataagt tggagtcatt acccaattat gatagaattt
    16861 acaagctata aggttattgt cctgggtttc aagcattagt ccatgcaagt ttttatgctt
    16921 tgcccattct atagatatat tgataagcgc gctgcctatg ccttgccccc tgaaatcctt
    16981 acatacggcg atatcttcta tataaaagat atattatctt atcagtattg tcaatatatt
    17041 caaggcaatc tgcctcctca tcctcttcat cctcttcgtc ttggtagctt tttaaatatg
    17101 gcgcttcata gagtaattct gtaaaggtcc aattctcgtt ttcatacctc ggtataatct
    17161 tacctatcac ctcaaatggt tcgctgggtt tatcgcaccc ccgaacacga gcacggcacc
    17221 cgcgaccact atgccaagaa tgcccaaggt aaaaattgcc ggccccgcca tgaagtccgt
    17281 gaatgccccg acggccgaag tgaagggcag gccgccaccc aggccgccgc cctcactgcc
    17341 cggcacctgg tcgctgaatg tcgatgccag cacctgcggc acgtcaatgc ttccgggcgt
    17401 cgcgctcggg ctgatcgccc atcccgttac tgccccgatc ccggcaatgg caaggactgc
    17461 cagcgctgcc atttttgggg tgaggccgtt cgcggccgag gggcgcagcc cctgggggga
    17521 tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc cccttcggcg
    17581 tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac aaggtttata aatattggtt
    17641 taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa
    17701 tgctggattt tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc cctcatctgt
    17761 cagcactctg cccctcaagt gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc
    17821 tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac
    17881 tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg ccagctccac gtcgccggcc
    17941 gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt
    18001 caacgtccgc ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc
    18061 gggtgtctcg cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc
    18121 gccagcccag cggcgagggc aaccagcccg g
//
